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Er:YAG Laser and Cyclosporin A Effect on Cell Cycle Regulation of Human Gingival Fibroblast Cells

Hojjat-Allah Abbaszadeh, Ali Asghar Peyvandi, Yousef Sadeghi, Akram Safaei, Mona Zamanian-Azodi, Maryam Sadat Khoramgah, Mostafa Rezaei-Tavirani
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Abstract

Introduction: Periodontitis is a set of inflammatory disorders characterized by periodontal attachment loss and alveolar bone resorption. Because of deficiency in periodontitis mechanical therapy, this study was aimed to explore the molecular influence of the erbium-doped: yttrium aluminum garnet (Er:YAG) laser and cyclosporin A (CsA) on human gingival fibroblasts (HGFs) for improvement in periodontal diseases therapy.
Methods: We focused on articles that studied the proteome profiles of HGFs after treatment with laser irradiation and application of CsA. The topological features of differentially expressed proteins were analyzed using Cytoscape Version 3.4.0 followed by module selection from the protein-protein interaction (PPI) network using Cluster ONE plugin. In addition, we performed gene ontology (GO) enrichment analysis for the densely connected region and key proteins in both PPI networks.
Results: Analysis of PPI network of Er:YAG laser irradiation on HGFs lead to introducing YWHAZ, VCP, HNRNPU, YWHAE, UBA52, CLTC, FUS and IGHG1 as key proteins while similar analysis revealed that ACAT1, CTSD, ALDOA, ANXA2, PRDX1, LGALS3, ARHGDI and EEF1A1 are the crucial proteins related to the effect of drug. GO enrichment analysis of hub-bottleneck proteins of the 2 networks showed the different significant biological processes and cellular components. The functional enrichments of module of Er:YAG laser network are included as fatty acid transmembrane transport, cytokinesis, regulation of RNA splicing and asymmetric protein localization. There are not any significant clusters in network of HGF treated by CsA.
Conclusion: The results indicate that there are 2 separate biomarker panels for the 2 treatment methods.

Keywords

Er:YAG laser; cyclosporin A (CsA); Protein-protein interaction (PPI) network analysis; Gene ontology; human fibroblast cell.

References

Apsey DJ, Kaciroti N, Loesche WJ. The diagnosis of periodontal disease in private practice. J Periodontol. 2006;77(9):1572-1581.

Flemmig TF. Periodontitis. Ann Periodontol. 1999;4(1):32- 37.

Ozden FO, Ozgönenel O, Ozden B, Aydogdu A. Diagnosis of periodontal diseases using different classification algorithms: a preliminary study. Niger J Clin Pract. 2015;18(3):416-421.

Genco RJ, Van Dyke TE. Prevention: reducing the risk of CVD in patients with periodontitis. Nat Rev Cardiol. 2010;7(9):479-480. doi:10.1038/nrcardio.2010.120.

Ahmed U, Tanwir F. Association of periodontal pathogenesis and cardiovascular diseases: a literature review. Oral Health Prev Dent. 2015;13(1):21-27. doi: 10.3290/j.ohpd.a32823

Oda S, Nitta H, Setoguchi T, Izumi Y, Ishikawa I. Current concepts and advances in manual and power‐driven instrumentation. Periodontol 2000. 2004;36(1):45-58.

Krohn-Dale I, Boe OE, Enersen M, Leknes KN. Er:YAG laser in the treatment of periodontal sites with recurring chronic inflammation: a 12-month randomized, controlled clinical trial. J Clin Periodontol. 2012;39(8):745-52. doi: 10.1111/j.1600-051X.2012.01912.x.

Strauss RA, Fallon SD. Lasers in contemporary oral and maxillofacial surgery. Dent Clin North Am. 2004;48(4):861- 888.

Passanezi E, Damante CA, DeRezende ML, Greghi SL. Lasers in periodontal therapy. Periodontol 2000. 2015;67(1):268-291.

Crespi R, Capparè P, Toscanelli I, Gherlone E, Romanos GE. Effects of Er: YAG laser compared to ultrasonic scaler in periodontal treatment: a 2-year follow-up split-mouth clinical study. J Periodontol. 2007;78(7):1195-1200.

Jung JY, Kang GC, Jeong YJ, Kim SH, Kwak YG, Kim WJ. Proteomic analysis in cyclosporin A-induced overgrowth of human gingival fibroblasts. Biol Pharm Bull. 2009;32(8):1480-5.

Sobral LM, Aseredo F, Agostini M, et al. Molecular events associated with ciclosporin A-induced gingival overgrowth are attenuated by Smad7 overexpression in fibroblasts. J Periodontal Res. 2012;47(2):149-158.

Ponnaiyan D, Jegadeesan V. Cyclosporine A: novel concepts in its role in drug-induced gingival overgrowth. Dent Res J (Isfahan). 2015;12(6):499-506.

Williamson MS, Miller EK, Plemons J, Rees T, Iacopino AM. Cyclosporine A upregulates interleukin-6 gene expression in human gingiva: possible mechanism for gingival overgrowth. J Periodontol. 1994;65(10):895-903.

Mitic K, Popovska M, Pandilova M, Jovanovic R, Spasovski G. The role of inflammation and apoptosis in cyclosporine A – induced gingival overgrowth. Bosn J Basic Med Sci. 2013;13(1):14–20.

Bartold P. Cyclosporine and gingival overgrowth. J Oral Pathol Med. 1987;16(9):463-468.

Song L, Yao J, He Z, Xu B. Genes related to inflammation and bone loss process in periodontitis suggested by bioinformatics methods. BMC Oral Health. 2015;15:105. doi:10.1186/s12903-015-0086-7.

Ara T, Kurata K, Hirai K, et al. Human gingival fibroblasts are critical in sustaining inflammation in periodontal disease. J Period Res. 2009;44(1):21-27.

Lee I-k, Lee M-j, Jang H-S. The interrelationship between human gingival fibroblast differentiation and cultivating time. Tissue Eng Regen Med. 2013;10(2):60-64.

Tavirani MR. Meningioma protein-protein interaction network. Arch Iran Med. 2014;17(4):262.

Safaei A, Tavirani MR, Oskouei AA, Azodi MZ, Mohebbi SR, Nikzamir AR. Protein-protein interaction network analysis of cirrhosis liver disease. Gastroenterol Hepatol Bed Bench. 2016;9(2):114.

Lackey B, Nunes QM, Higham SM, Fernig DG, Valappil SP. In silico analyses of heparin binding proteins expression in human periodontal tissues. BMC Res Notes. 2016;9:53. doi: 10.1186/s13104-016-1857-1.

Zamanian-Azodi M, Rezaei-Tavirani M, Rahmati-Rad S, Hasanzadeh H, Tavirani MR, Seyyedi SS. Protein-protein interaction network could reveal the relationship between the breast and colon cancer. Gastroenterol Hepatol Bed Bench. 2015;8(3):215.

Safari-Alighiarloo N, Taghizadeh M, Rezaei-Tavirani M, Goliaei B, Peyvandi AA. Protein-protein interaction networks (PPI) and complex diseases. Gastroenterol Hepatol Bed Bench. 2014;7(1):17-31.

Bakail M, Ochsenbein F. Targeting protein–protein interactions, a wide open field for drug design. C R Chim. 2016;19(1):19-27. doi:10.1016/j.crci.2015.12.004

Zamanian–Azodi M, Rezaei–Tavirani M, Hasanzadeh H, et al. Introducing biomarker panel in esophageal, gastric, and colon cancers; a proteomic approach. Gastroenterol Hepatol Bed Bench. 2015;8(1):6-18.

Ballatore C, Brunden KR, Trojanowski JQ, Lee VM, Smith AB 3rd, Huryn DM. Modulation of protein-protein interactions as a therapeutic strategy for the treatment of neurodegenerative tauopathies. Curr Top Med Chem. 2011;11(3):317-30.

Ogita M, Tsuchida S, Aoki A, et al. Increased cell proliferation and differential protein expression induced by low-level Er: YAG laser irradiation in human gingival fibroblasts: proteomic analysis. Lasers Med Sci. 2015;30(7):1855-1866. doi: 10.1007/s10103-014-1691-4.

Shannon P, Markiel A, Ozier O, et al. Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res. 2003;13(11):2498-2504.

Proulx SR, Promislow DE, Phillips PC. Network thinking in ecology and evolution. Trends Ecol Evol. 2005;20(6):345- 353.

Bertoldi C, Bellei E, Pellacani C, et al. Non‐bacterial protein expression in periodontal pockets by proteome analysis. J Clini Periodontol. 2013;40(6):573-582. doi:10.1111/ jcpe.12050.

Xia J, Benner MJ, Hancock RE. NetworkAnalyst-- integrative approaches for protein-protein interaction network analysis and visual exploration. Nucleic Acids Res. 2014;42(Web Server issue):W167-W74. doi:10.1093/nar/ gku443.

Yang J, Fuller PJ, Morgan J, et al. Use of phage display to identify novel mineralocorticoid receptor-interacting proteins. Mol Endocrinol. 2014;28(9):1571-1584. doi: 10.1210/me.2014-1101.

Hamrita B, Nasr HB, Hammann P, et al. An elongation factor-like protein (EF-Tu) elicits a humoral response in infiltrating ductal breast carcinomas: an immunoproteomics investigation. Clin Biochem. 2011;44(13):1097-1104.

Morrissey C, Schwefel D, Ennis-Adeniran V, Taylor IA, Crow YJ, Webb M. The eukaryotic elongation factor eEF1A1 interacts with SAMHD1. Biochem J. 2015;466(1):69-76. doi:10.1042/BJ20140203.

Liu H, Ding J, Chen F, et al. Increased expression of elongation factor-1α is significantly correlated with poor prognosis of human prostate cancer. Scand Urol Nephrol. 2010;44(5):277-283. doi:10.3109/00365599.2010.492787.

Ye J, Beetz N, O’Keeffe S, et al. hnRNP U protein is required for normal pre-mRNA splicing and postnatal heart development and function. Proc Natl Acad Sci. 2015;112(23):E3020-E3029. doi:10.1073/pnas.1508461112.

Bao K, Belibasakis GN, Selevsek N, Grossmann J, Bostanci N. Proteomic profiling of host-biofilm interactions in an oral infection model resembling the periodontal pocket. Sci Rep. 2015;5:15999. doi:10.1038/srep15999.

Aleksic V, Aoki A, Iwasaki K, et al. Low-level Er: YAG laser irradiation enhances osteoblast proliferation through activation of MAPK/ERK. Lasers Med Sci. 2010;25(4):559- 569.

Pourzarandian A, Watanabe H, Ruwanpura SM, Aoki A, Ishikawa I. Effect of low-level Er: YAG laser irradiation on cultured human gingival fibroblasts. J Periodontol. 2005;76(2):187-193.

Becker M, Kuhse J, Kirsch J. Effects of two elongation factor 1A isoforms on the formation of gephyrin clusters at inhibitory synapses in hippocampal neurons. Histochem Cell Biol. 2013;140(6):603-609.

Brett P, Parkar M, Olsen I, Tonetti M. Expression profiling of periodontal ligament cells stimulated with enamel matrix proteins in vitro: a model for tissue regeneration. J Dent Res. 2002;81(11):776-783.

Boulter E, Garcia-Mata R, Guilluy C, et al. Regulation of Rho GTPase crosstalk, degradation and activity by RhoGDI1. Nat Cell Biol. 2010;12(5):477-483. doi: 10.1038/ ncb2049.

Califice S, Castronovo V, Van Den Brûle F. Galectin-3 and cancer (Review). Int J Oncol. 2004;25(4):983-992.

Zhang P, Liu B, Seo MS, Rhee SG, Obeid LM. Thioredoxin peroxidase is a novel inhibitor of apoptosis with a mechanism distinct from that of Bcl-2. J Biol Chem. 1997;272(49):30615-30618.

Barbieri E, Di Fiore PP, Sigismund S. Endocytic control of signaling at the plasma membrane. Curr Opin Cell Biol. 2016;39:21-27. doi:10.1016/j.ceb.2016.01.012.

Fritsche KL. The science of fatty acids and inflammation. Adv Nut. 2015;6(3):293S-301S. doi:10.3945/an.114.006940.

Masi LN, Rodrigues AC, Curi R. Fatty acids regulation of inflammatory and metabolic genes. Curr Opin Clin Nut Metab Care. 2013;16(4):418-424. doi:10.1097/ MCO.0b013e32836236df.




DOI: https://doi.org/10.22037/2010.v8i3.16363