• Logo
  • SBMUJournals

Effect of Laser Irradiation on Cell Cycle and Mitosis

Monireh Ganjali, Alexander M. Seifalian, Masoud Mozafari
167

Views

PDF

Abstract

Introduction: In this research, low-level helium-neon (He-Ne) laser irradiation effects on monkey kidney cells (Vero cell line) mitosis were studied.
Methods: The experiment was carried out on a monkey kidney cell line “Vero (CCL-81)”. This is a lineage of cells used in cell cultures and can be used for efficacy and media testing. The monolayer cells were formed on coating glass in a spectral cuvette (20×20×30 mm). The samples divided into two groups. The first groups as irradiated monolayer cells were exposed by a He-Ne laser (PolyaronNPO, L’vov, Ukraine) with λ = 632.8 nm, max power density (P) = 10 mW/cm2, generating linearly polarized and the second groups as the control monolayer cells were located in a cuvette protected by a lightproof screen from the first cuvette and also from the laser exposure. Then, changing functional activity of the monolayer cells, due to the radiation influence on some physical factors were measured.
Results: The results showed that low-intensity laser irradiation in the range of visible red could make meaningful changes in the cell division process (the mitosis activity). These changes depend on the power density, exposure time, the presence of a magnetic field, and the duration of time after exposure termination. The stimulatory effects on the cell division within the power density of 1-6 mW/(cm2) and exposure time in the range of 1-10 minutes was studied. It is demonstrated that the increase in these parameters (power density and exposure time) leads to destructing the cell division process.
Conclusion: The results are useful to identify the molecular mechanisms caused by low-intensity laser effects on the biological activities of the cells. Thus, this study helps to optimize medical laser technology as well as achieving information on the therapeutic effects of low-intensity lasers.

Keywords

Low-intensity laser irradiation; Power density; Mitosis activity; Laser therapy

References

Yumura S. A novel low-power laser-mediated transfer of foreign molecules into cells. Sci Rep. 2016;6:22055. doi:10.1038/srep22055

Akhavan O, Ghaderi E, Shirazian SA. Near infrared laser stimulation of human neural stem cells into neurons on graphene nanomesh semiconductors. Colloids Surf B Biointerfaces. 2015;126:313-321. doi:10.1016/j. colsurfb.2014.12.027

Moon BJ, Lee HY, Kim KN, et al. Experimental Evaluation of Percutaneous Lumbar Laser Disc Decompression Using a 1414 nm Nd:YAG Laser. Pain Physician. 2015;18(6):E1091-1099.

Terhorst D, Fossum E, Baranska A, et al. Laser-assisted intradermal delivery of adjuvant-free vaccines targeting XCR1+ dendritic cells induces potent antitumoral responses. J Immunol. 2015;194(12):5895-5902. doi:10.4049/jimmunol.1500564

Tuner J, Hode L, Nobel A. Laser therapy: clinical practice and scientific background: a guide for research scientists, doctors, dentists, veterinarians and other interested parties within the medical field. Prima Books; 1999.

Injushin YM, Chekurov PR. Biostimulation of the bioplasma by laser beams. Alma Ata, Kazakhatan; 1975:117.

Aronowitz JN. The Other Radiation Therapy. Int J Radiat Oncol Biol Phys. 2015;93(2):227-228. doi:10.1016/j. ijrobp.2015.04.041

Mester A. Laser Biostimulation. Photomed Laser Surg 2013;31(6):237-239. doi:10.1089/pho.2013.9876

Wu JY, Chen CH, Wang CZ, Ho ML, Yeh ML, Wang YH. Low-power laser irradiation suppresses inflammatory response of human adipose-derived stem cells by modulating intracellular cyclic AMP level and NF-kappaB activity. PLoS One. 2013;8(1):e54067. doi:10.1371/journal. pone.0054067

Wu JY, Chen CH, Yeh LY, Yeh ML, Ting CC, Wang YH. Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate. Int J Oral Sci. 2013;5(2):85-91. doi:10.1038/ijos.2013.38

Karu T. Photobiology of low-power laser effects. Health Phys. 1989;56(5):691-704.

Gao X, Xing D. Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci. 2009;16:4. doi:10.1186/1423-0127-16-4

Hawkins D, Abrahamse H. Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. Photomed Laser Surg. 2006;24(6):705-714. doi:10.1089/pho.2006.24.705

Hawkins DH, Abrahamse H. The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation. Lasers Surg Med. 2006;38(1):74-83. doi:10.1002/lsm.20271

Smith K. The photobiological basis of low level laser radiation therapy. Laser Ther. 1991;3(1):19-24. doi:10.5978/ islsm.91-OR-03

Ohshiro T, Calderhead RG. Low level laser therapy: A practical introduction. John Wiley & Sons; 1988. doi:10.1002/bjs.1800760445

Boulnois JL. Photophysical processes in recent medical laser developments: A review. Lasers Med Sci. 1986;1(1):47- 66. doi:10.1007/bf02030737

Fenyo M. Theoretical and experimental basis of biostimulation by laser irradiation. Opt Laser Technol. 1984;16(4):209-215. doi:10.1016/0030-3992(84)90029-X

Khoo IC. Cholesteric, Smectic, and Ferroelectric Liquid Crystals, in Liquid Crystals. 2nd ed. Wiley; 2007. doi:10.1002/9780470084038.ch4

Simoni F. Nonlinear optical properties of liquid crystals and polymer dispersed liquid crystals. World Scientific Pub Co Inc; 1997. doi:10.1142/2343

Brill G. Molecular and cellular basis of therapeutic action of low - intensity laser radiation. Acad Saratov Med Univer; 2000.

Sullins KE. Lasers and wound healing: Practical uses. Clinical Techniques in Equine Practice. 2004;3(2):182-187. doi:10.1053/j.ctep.2004.10.001

KaruTI. Low-Power Laser Effects, in Lasers in Medicine. CRC Press; 2001.

Kondo T, Hayashi S. Mitotic cell rounding accelerates epithelial invagination. Nature. 2013;494(7435):125-129. doi:10.1038/nature11792

Hillenkamp F. Lasers in biology and medicine. Springer Science & Business Media; 2013.

Parvin P, Dehghanpour HR, Moghadam MS, Daneshafrooz V. Validity of reciprocity rule on mouse skin thermal damage due to CO2 laser irradiation. Opt Spectrosc. 2013;115(1):147-154. doi:10.1134/s0030400x13070138

Wang X, Zgadzaj R, Fazel N, et al. Quasi-monoenergetic laser-plasma acceleration of electrons to 2 GeV. Nat Commun. 2013;4:1988. doi:10.1038/ncomms2988

Wang L, Li J, Pan J, et al. Revealing the binding structure of the protein corona on gold nanorods using synchrotron radiation-based techniques: understanding the reduced damage in cell membranes. J Am Chem Soc. 2013;135(46):17359-17368. doi:10.1021/ja406924v




DOI: https://doi.org/10.22037/jlms.v9i4.12497