• Logo
  • SBMUJournals

The effect of combination of red, infrared and blue wavelengths of Low Level Laser on reduction of abdominal girth circumference: a before - after case series

Katayoon Montazeri, Soheila Mokmeli, Maryam Barati



Introduction: The objective of this study was to assess the efficacy of a combination of three wavelengths (including red, infra-red, and blue) of low-level laser (LLL) as a non-invasive therapeutic method to reduce abdominal girth. To achieve biochemical activity on adipocytes, a red laser was used in our selective laser combination. Near-infrared laser was used to increase depth of penetration. Nitrosyl complexes of hemoglobin (NO-Hb) are sensitive to blue light, thereby leading to increase in release of biologically active nitric oxide (NO), which can affect tissue perfusion. Thus, a blue LED was added to the laser combination.

Methods: [W1] Eighteen females participated in the study. Twelve sessions of laser therapy were performed, two sessions per week for each subject. Continuous wave diode lasers, including red (630nm), infra-red (808nm), and a blue LED (450nm) were applied and were all designed by the Canadian Optic and Laser Center.

Results: Statistical analyses revealed that upper abdomen size significantly decreased from pre- (91.86 ± 11.16) to post- (87.41 ± 10.52) low level laser therapy (LLLT) (p<0.001). Middle abdomen size showed significant reduction from pre- (97.02 ± 8.82) to post- (91.97 ± 8.49) LLLT (p<0.001). Lower abdomen size significantly decreased from pre- (100.36 ± 9.45) to post- (95.80 ± 8.52) LLLT (p<0.001).

Conclusion: Based on this case series pilot investigation, the combination of 3 different wavelengths of LLL was effective for abdominal girth reduction in 100% of our subjects (p<0.001), without any side effects. Future studies will assess the long-term benefits of this laser combination for reduction of subcutaneous fat deposits.



Diode lasers; low level laser therapy; non-invasive girth reduction; Non invasive.


Rossi AB, Vergnanini AL. Cellulite: a review. J Eur Acad Dermatol Venereol. 2000; 14 (4): 251–62. doi:10.1046/j.1468-3083.2000.00016.x. PMID 11204512.

PINNA K. Nutrition and diet therapy. 2007. Belmont, CA: Wadsworth. p.178

Emanuele E, Bertona M, Geroldi D. A multilocus candidate approach identifies ACE and HIF1A as susceptibility genes for cellulite. J Eur Acad Dermatol Venereol. 2010; 24 (8): 930–5. doi:10.1111/j.1468-3083.2009.03556.x. PMID 20059631.

- Jackson RF, Roche GC, Shanks SC. Double-blind, placebo-controlled randomized trial evaluating the ability of low-level laser therapy to improve the appearance of cellulite". Lasers Surg Med. 2013; 45(3): 141–7.

Wanner M, Avram M. An evidence-based assessment of treatments for cellulite. J Drugs Dermatol. 2008; 7 (4): 341–5. PMID 18459514.

Pavicic T, Borelli C, Korting HC. Cellulite--the greatest skin problem in healthy people?An approach. J Dtsch Dermatol Ges. 2006; 4 (10): 861–70. doi:10.1111/j.1610-0387.2006.06041.x. PMID 17010177.

Terranova F, Berardesca E, Maibach H. Cellulite: nature and aetiopathogenesis. Int J Cosmet Sci. 2006; 28 (3): 157–67. doi:10.1111/j.1467-2494.2006.00316.x. PMID 18489272.

Mulholland RS, Paul M, Chalfoun C. Noninvasive body contouring with radiofrequency, ultrasound, cryo-lipolysis, and low-level laser therapy. Clin Plastic Surg. 2011; 38:503–20. 2011.

Jackson, R., Roche, G., Butterwick, JK. et al. " Low level laser-assisted liposuction: clinical trial of its effectiveness for enhancing ease of liposuction procedures and facilitating the recovery process for patients undergoing thigh, hip and stomach contouring. Am J Cosmet Surg. 2004.

Maloney R, Shanks S, Jenney E. The reduction in cholesterol and triglyceride serum levels following low-level laser irradiation: a non-controlled, non-randomized pilot study. Laser Surg Med. 2009; 21S:66..

Avci P, Nyame TT, Gupta GK, Sadasivam M, Hamblin MR. Low-Level Laser Therapy for Fat Layer Reduction: A Comprehensive Review. Laser Surg Med. 2013; 45(6); 349-57. doi: 10. 1002/lsm.22153

Jackson FR, Roche GC, Wisler K. Reduction in cholesterol and triglycerides serum levels following low level laser irradiation: A noncontrolled, nonrandomized pilot study. Am J Cosmet Surg. 2010; 27(4):177- 84.

Rushdi TA. Effect of low-level laser therapy on cholesterol and triglyceride levels in ICU patients: A controlled randomized study. EJCTA .2010; 4: 96-9.

Karu TI. Mitochondrial signaling in mammalian cells activated by red and near-IR radiation. Photochem Photobiol. 2008; 84:1091–9.

Karu TI. Photobiology of low-power laser effects. Health Phys. 1989; 56:691–704.

Karu TI, Afanasyeva NI. Cytochrome C oxidase as primary photo acceptor for cultured cells in visible and near IR regions. DokladyAkadNauk (Moscow). 1995; 342:693–5.

Rosenbaum M, Prieto V, Hellmer J, Boschmann M, Krueger J, Leibel RL, et al. An exploratory investigation of the morphology and biochemistry of cellulite. Plast Reconstr Surg. 1998; 101:1934–9.

Neira R, Arroyave J, Ramirez H, Ortiz CL, Solarte E, Sequeda F, et al. Fat liquefaction: effect of low level laser energy on adipose tissue. Plast Reconstr Surg. 2002; 110:912–22. doi: 10.1097/00006534-200209010-00030.

Lucassen GW, van der Sluys WL, van Herk JJ, Nuijs AM, Wierenga PE, Barel AO, et al. The effectiveness of massage treatment on cellulite as monitored by ultrasound imaging. Skin Res Technol. 1999; 3:154–60.

Lach E, Pap S. Laser treatment for cellulite: A non-invasive alternative to liposuction. Lasers Surg Med. 2004; 34, S16–32.

Bourassa DJ, et al. DCA population within a species that has distinct genetic variation; as published at: www.ChiroEco.com. 2010.

Jackson RF, Dedo DD, Roche GC, Turok DI, Maloney RJ. Low-Level Laser Therapy as a Non-Invasive Approach for Body Contouring: A Randomized, Controlled Study. Lasers Surg Med. 2009;41:799–809.

Savoia A, Landi S, Vannini F, Baldi A. Low-Level Laser Therapy and Vibration Therapy for the Treatment of Localized Adiposity and Fibrous Cellulite. Dermatol Ther (Heidelb). 2013; 3(1): 41–52.

Nestor MS, Newburger J, Zarraga MB. Body Contouring Using 635-nm Low Level Laser Therapy. Semin Cutan Med Surg. 2013; 32:35-40.

McRae E, Boris J. Independent evaluation of low-level laser therapy at 635 nm for non-invasive body contouring of the waist, hips, and thighs. Lasers Surg Med. 2013; 45(1):1–7.

Nestor MS, Zarraga MB, Park H. Effect of 635 nm low-level lasertherapy on upper arm circumference reduction: A double-blind, randomized, sham-controlled trial. J Clin Aesthet Dermatol. 2012; 5:42-8.

Kolari PJ. Penetration of unfocused laser light into the skin". Arch Dermatol Res. 1985; 277:342–4. doi: 10.1007/BF00509097.

Kolarova H, Ditrichova D, Wagner J. Penetration of the laser light into the skin in vitro. Lasers Surg Med. 1999; 24:231–5. doi: 10.1002/ (SICI) 1096-9101.

Enwemeka CS. Attenuation and penetration of visible 632.8 nm and invisible infra-red 904 nm light in soft tissues. Laser Ther J. 2003; 13:16.

Smith AM, Mancini MC, Nie S. Bioimaging: Second window for in vivo imaging. Nature Nanotechnol. 2009; 4 (11): 710–1. doi:10.1038/nnano. 326. ISSN 1748-3387.

Mittermayr R, Osipov A, Piskernik C, Haindl S, Dungel P, Weber C, et al. Blue Laser Light Increases Perfusion of a Skin Flap Via Release of Nitric Oxide from Hemoglobin. Mol Med. 2007;13(1-2):22-9. doi:10.2119/2006-00035.

Kozlov AV, Costantino G, Sobhian B, Szalay L, Umar F, Nohl H, et al. Mechanisms of vasodi-latation induced by nitrite instillation in intestinal lumen: possible role of hemoglobin. Antioxid Redox Signal. 2005;7:515–21

Gladwin MT, Crawford JH, Patel RP. The biochemistry of nitric oxide, nitrite, and hemoglobin: role in blood flow regulation. Free Radic Biol Med. 2004;36:707–17.

Cosby K, Partovi KS, Crawford JH, Patel RP, Reiter CD, Martyr S, et al. Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat Med. 2003;9:1498–505.

DOI: https://doi.org/10.22037/jlms.v8i3.12385