A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells
Journal of Lasers in Medical Sciences,
Vol. 8 No. 3 (2017),
30 Shahrivar 2017
,
Page S46-S50
Abstract
Background: In recent years the use of low-power lasers has been widely used in medicine. With the introduction of affordable LED light, clinical application of LED light has become more and more popular. However, some researchers believe that because of the lack of coherence of the LED light, it can be different in biological effects in comparison to laser. In this study, we compared the biological effects of low level laser to those of LED light.
Methods: Human skin fibroblast cell line Hu02 was irradiated with low level laser and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through the analyzing of cell proliferation, production of reactive oxygen species within the cell and the rate of cell division.
Results: Our findings showed that production of reactive oxygen species within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more increment in comparison the LED light. The MTT results showed that laser light at low energy density (less than 5 joules per square centimeter) was increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm2 compared to the control group, but this increasing was not statistically significant.
Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in compared with LED light.
- Low level laser
- LED light
- Reactive oxygen species
- fibroblast cell
How to Cite
References
- Huang Y Y, Sharma S K, Carroll J, Hamblin M R, Biphasic dose response in low level light therapy-an update, Dose-Response, 9:602–618, 2011.
- Lizarelli RFZ, Lamano-Carvalho TL, Brentegani LG (1999) Histometrical evaluation of the healing of the dental alveolus in rats after irradiation with a low-powered GaAlAs laser. SPIE 3593:49–55
- Gasparyan VC (2000) Method of determination of aortic valve parameters for its reconstruction with autopericardium: an experimental study. J Thorac Cardiovasc Surg 119:386–387
- Kemmotsu O, Sato K, Furomido H, Harada K, Takigawa C, Kaseno S (1991) Efficacy of low reactive-level laser therapy for pain attenuation of postherpetic neuralgia. Laser Therapy 3:1–75
- Mester E, Mester AF, Mester A (1985) The biomedical effects of laser application. Lasers Surg Med 5:31–39
- Rochkind S, Rousso M, Nissan M, Villarreal M, Barr-Nea L, Rees DG (1989) Systemic effects of low-power laser irradiation on the peripheral and central nervous system, cutaneous wounds and burns. Lasers Surg Med 9:174–182
- Hawkins D, Abrakamse H (2005) Biological effects of helium-neon laser irradiation on normal and wounded human skin fibroblasts. Photomed Laser Sur 23:251–259
- Karu TI 1989. Laser biostimulation: a photobiological phenomenon. J Photochem Photobiol B 3(4): 638-640.
- Greco M, Guida G, Perlino E, Marra E and Quagliariello E 1989. Increase in RNA and protein synthesis by mitochondria irradiated with helium-neon laser. Biochem Biophys Res Commun 163(3): 1428-1434.
- Karu TI and Kolyakov SF 2005. Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg 23(4): 355-361.
- Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM and Cingolani A 1984. Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by helium-neon laser. FEBS Lett 175(1): 95-99.
- Andreyev A. Y., Kushnareva Y. E., Starkov A. A. Mitochondrial metabolism of reactive oxygen species. Biochemistry (Moscow) 2005;70:200–214.
- Turrens J. F. Mitochondrial formation of reactive oxygen species. J. Physiol. 2003;552:335–344.
- 5. Cadenas E., Davies K. J. Mitochondrial free radical generation, oxidative stress, and aging. Free Radical Biol. Med. 2000;29:222–230.
- Hawkins D, Abrahamse H (2006) Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. Photomed Laser Surg 24:705–714
- Hu WP, Wang JJ, Yu CL, Lan CCE, Chen GS, Yu HS (2007) Helium-Neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria. J Investigat Dermatol 127:2048–2057
- Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49:1–17
- Chen A C-H, Praveen R, Arany PR, Huang Y-Y, Tomkinson EM, Saleem T, et al. Low level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts. Proc SPIE. 2009; 7165: 71650B.
- Karin M and Lin A 2002. NF-kappaB at the crossroads of life and death. Nat Immunol 3(3): 221-227.
- Whelan HT, Houle JM, Whelan NT et al. (2000): The NASA Light-Emitting Diode Medical Program-Progress in Space Flight and Terrestrial Applications. Space Tech. & App. Int’l. Forum. 504:37-43.
- Smith KC (2010): Laser and LED photobiology. Laser Therapy, 19: 72-78.
- Chen AC, Arany PR, Huang YY, et al. Low-level laser therapy activates NF-kB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS One 2011;6: e22453.
- Wan, X.S.; Zhou, Z.; Kennedy, A.R. Adaptation of the dichlorofluorescein assay for detection of radiation-induced oxidative stress in cultured cells. Radiat. Res. 2003, 160, 622–630.
- Jahangiri Noudeh Y, Shabani M, Vatankhah N, Hashemian SJ, Akbari K. A combination of 670 and 810nm diode lasers for wound healing acceleration in diabetic rats. Photomed Laser Surg. 2010;28:621-7.
- Prindeze NJ, Moffatt LT, Shupp JW. Mechanisms of action for light therapy: a review of molecular interactions. Exp Biol Med (Maywood). 2012;237:1241-8.
- Bhide SA, Nutting CM. Recent advances in radiotherapy. BMC Med 2010;8:25.
- Miura Y. Oxidative stress, radiation-adaptive responses, and aging. J Radiat Res 2004;45:357–372.
- Abstract Viewed: 1221 times
- PDF Downloaded: 541 times