ISSN: 1735-1308
2019
Administration of Nicotine Exacerbates the Quinine-induced Structural and Functional Alterations of Testicular Tissue in Adult Rats: An Experimental Study
Urology Journal,
2019,
5 January 2019
,
Page 5884
https://doi.org/10.22037/uj.v16i7.5884
Abstract
Purpose: In this study the role of nicotine (NCT) administration on the intensity of rat testicular tissue alterations induced by quinine (QU) was evaluated.
Materials and Methods: Forty adult Wistar rats were divided into four groups. Control (CON), NCT administrated (4 mg/kg) (NCT), QU treated (25 mg/kg for 7 days) (QU), and nicotine with quinine received (NCT+QU). After 28 days, serum testosterone and malondialdehyde (MDA) levels were measured. Testes and epididymides samples were prepared for determining tissue MDA levels, histomorphometry, microscopic indices of spermatogenesis, immunohistochemistry of p53 and sperm analysis.
Results: Testosterone levels were decreased significantly (P=.0004) in treated groups compared to CON group. Serum MDA levels were increased significantly (P=.0004) in NCT and QU groups compared to CON group. Tissue MDA levels were increased significantly (P=.0012) in NCT+QU group in comparison to CON group. These parameters were changed significantly in NCT+QU group compared to QU group. Seminiferous tubules diameter was decreased significantly (P < .0001) in treated groups compared to CON group and in NCT+QU group compared to QU group. The height of germinal epithelium was decreased significantly (P=.0001) in NCT and NCT+QU groups compared to CON and QU groups. The number of Sertoli cells, spermatocytes and spermatids was decreased significantly in treated groups compared to CON group. The number of spermatogonia was decreased significantly (P=.0017) in NCT and NCT+QU groups compared to CON group. The number of Sertoli cells, spermatogonia and spermatocytes was decreased significantly in NCT+QU group compared to QU group. All indices of spermatogenesis were decreased in treated groups compared to CON group. The lowest mean of these indices was observed in NCT+QU group. The sperm viability was decreased significantly (P < .0001) in treated groups compared to CON group. Sperm count and motility were decreased significantly in NCT and NCT+QU groups compared to CON group. All experimental groups showed the over-expression of p53 compared to CON group.
Conclusion: The administration of nicotine could be involved in the exacerbation of testicular tissue alterations related to quinine therapy.
- nicotine
- quinine
- rats
- structural and functional alterations
- testicular tissue
References
Guerriero G, Trocchia S, Abdel-Gawad FK, Ciarcia G. Roles of reactive oxygen species in the spermatogenesis regulation. Front Endocrinol (Lausanne). 2014; 5:56.
Zenzes MT. Smoking and reproduction: gene damage to human gametes and embryos. Hum Reprod Update. 2000; 6:122-31.
Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev. 2008, 1:15-24.
Arabi M. Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia. 2004; 36:305-10.
Oyeyipo IP, Raji Y, Emikpe BO, Bolarinwa AF. Effects of nicotine on sperm characteristics and fertility profile in adult male rats: a possible role of cessation. J Reprod Infertil. 2011; 12:201-7.
Ahmadnia H, Ghanbari M, Moradi MR, Khaje-Dalouee M. Effect of cigarette smoke on spermatogenesis in rats. Urol J. 2007; 4:159-63.
Bolat SM, Akdeniz E, Ozkaya S, et al. Smoking and Lower Urinary Tract Symptoms. Urol J. 2015; 12:2447-51.
Hassan A, Abo-Azma SM, Fayed S, Mostafa T. Seminal plasma cotinine and insulin-like growth factor-I in idiopathic oligoasthenoteratozoospermic smokers. BJU Int. 2009; 103:108-11.
Kremsner PG, Zotter GM, Bach M, Graninger W. A case of transient organic brain syndrome during quinine treatment. Rev Soc Bras Med Trop. 1989; 22:53.
Izaguirry AP, Pavin NF, Soares MB, et al. Effect of quinine-loaded polysorbate-coated nanocapsules on male and female reproductive systems of rats. Toxicol Res. 2016; 5:1561–72.
Farombi EO, Ekor M, Adedara IA, Tonwe KE, Ojujoh TO, Oyeyemi MO. Quercetin protects against testicular toxicity induced by chronic administration of therapeutic dose of quinine sulfate in rats. J Basic Clin Physiol Pharmacol. 2012; 23:39–44.
Nesseim WH, Haroun HS, Mostafa E, Youakim MF, Mostafa T. Effect of nicotine on spermatogenesis in adult albino rats. Andrologia. 2011; 43:398-404.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351–58.
Shetty G, Wilson G, Huhtaniemi I, Shuttlesworth GA, Reissmann T, Meistrich ML. Gonadotropin-releasing hormone analogs stimulate and testosterone inhibits the recovery of spermatogenesis in irradiated rats. Endocrinology. 2000; 141:1735-45.
Zambrano E, Rodriguez-Gonzalez GL, Guzman C, et al. A maternal low protein diet during pregnancy and lactation in the rat impairs male reproductive development. J Physiol. 2005; 563(Pt 1):275-84.
Jin L, Xue HY, Jin LJ, Li SY, Xu YP. Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes. Eur J Pharmacol. 2008; 582:162-67.
Wyrobek AJ, Gordon LA, Burkhart JG, et al. An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals: A report of the US environmental protection agency gene-tox program. Mutat Res. 1983; 115:1-72.
Azad F, Nejati V, Shalizar-Jalali A, Najafi G, Rahmani F. Royal jelly protects male mice against nicotine-induced reproductive failure. Vet Res Forum. 2018; 9:231-38.
Agarwal A, Makker K, Sharma R. Clinical Relevance of Oxidative Stress in Male Factor Infertility: An Update. Am J Reprod Immunol. 2008; 59:2-11.
Osinubi AA, Noronha CC, Okanlawon AO. Morphometric and stereological assessment of the effects of long-term administration of quinine on the morphology of rat testis. West Afr J Med. 2005; 24:200–5.
Walker WH, Cheng J. FSH and testosterone signaling in Sertoli cells. Reproduction. 2005; 130:15-28.
Zhang QX, Zhang XY, Zhang ZM, et al. Identification of testosterone-/androgen receptor-regulated genes in mouse Sertoli cells. Asian J Androl. 2012; 14:294–300.
Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003; 79:829–43.
Shen HM, Ong CN. Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility. Free Radic Biol Med. 2000; 28:529-36.
Li M, He Y, Dubois W, Wu X, Shi J, Huang J. Distinct regulatory mechanisms and functions for p53- activated and p53-repressed DNA damage response genes in embryonic stem cells. Mol Cell. 2012; 46:30-42.
Yin Y, Stahl DE, Wolf BC, Morgentaler A. P53 and Fas are sequential mechanisms of testicular germ cell apoptosis. J Androl. 2002; 23:64–70.
Mohammad Ghasemi F, Faghani F, Fallah Karkan M. The Protective Effect of Melatonin on Sperm Parameters, Epididymis and Seminal Vesicle Morphology in Adult Mouse Treated With Busulfan. J Iran Anatomic Sci. 2010; 8:25-36.
Crissman JW, Goodman DG, Hildebrandt PK, et al. Best practices guideline: toxicologic histopathology. Toxicol Pathol. 2004, 32:126-31.
Katoh C, Kitajima S, Saga Y, Kanno J, Horii I, Inoue T. Assessment of quantitative dual-parameter flowcytometric analysis for the evaluation of testicular toxicity using cyclophosphamide- and ethinylestradiol-treated rats. J Toxicol Sci. 2002; 27:87-96.
- Abstract Viewed: 0 times
- Just Accepted/5884 Downloaded: 0 times
