International Clinical Neuroscience Journal

Vol. 7 No. 4 (2020)

Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study

International Clinical Neuroscience Journal, Vol. 7 No. 4 (2020), 28 Mehr 2020 , Page 190-195

Abstract

Background: Background: Botulinum toxin A (BtxA) is a powerful neurotoxin reported to be effective as a cancer adjuvant therapy with fewer side effects. Previously we showed the apoptotic effects of BtxA on the GBM cell line (U87-MG). In order to confirm the positive neurotoxicity of BtxA on other cancer cell lines, including SK-OV-3, CHO, MCF-7, and PC-3, the present research has designed.
Methods: The cell lines prepared, cultured, and exposed to different concentrations of BtxA for 24 and 48 hours. Using MTT, Annexin V/PI assays and western blotting, cell viability, and apoptosis studied.
Results: Our results showed that different BtxA Botox concentrations led to significant cell death in each cell line in a dose-dependent manner (P < 0.001) but did not for PC-3 cells. The results of the Annexin V/PI staining indicated that BtxA induced apoptosis after 24 hours. The 1.45U, 1.75U, and 1.65U of BtxA respectively induced more than 50% apoptosis in MCF 7, SK-OV-3, and CHO cell lines. The results of caspase 3 showed more protein expression in the treated group compares to the control group.
Conclusion: BtxA, as a neurotoxin, can insert therapeutic anti-cancer and apoptotic effects on various types of cancerous cells; further studies need to illuminate the possible mechanisms.

Keywords:
  • BtxA neurotoxicity
  • Apoptosis
  • MCF-7
  • PC-3
  • SK-OV3
  • CHO cell lines

How to Cite

1.
Farhadi M, Jameie M, Derakhshanmehr B, Tahmasebi M, Soleimani M, Jameie SB. Neurotoxin Botulinum Inserts Apoptotic Effects on Certain Cancer Cell Lines Via Neural Niche of Tumors: A Molecular Study. Int Clin Neurosci J [Internet]. 2020 Sep. 28 [cited 2024 Sep. 13];7(4):190-5. Available from: https://journals.sbmu.ac.ir/neuroscience/article/view/32419

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