In Vitro Assessment of Synthetic Nano Engineered Graft Designed for Further Clinical Study in Nerve Regeneration
International Clinical Neuroscience Journal,
Vol. 5 No. 3 (2018),
30 September 2018
,
Page 86-91
Abstract
Background: Electrospun nanofibrous scaffolds are considered as promising candidates in neural tissue regeneration due to their ability to support neural cell attachment, spreading and proliferation.
Methods: In this paper, various type of nanofibers scaffold based on polycaprolactone) (PCL) were fabricated using electrospinning. The main drawback of PCL scaffolds is their low bioactivity of scaffold surface. To overcome this surface and composition modification was used to enhanced hydrophilicity and bioactivity of scaffold.
Results: The scanning electron microscopy (SEM) results indicate that fiber diameter entirely depends on the solvent system and added component of gelatin and chitosan which by adding gelatin and chitosan fiber diameter decreased. In vitro studies using PC12 cells revealed that the plasma surface modified and blended scaffold with chitosan and gelatin nanofibrous scaffold supports cell attachment, spreading and indicate a significant increase in proliferation of PC12 in the presence of chitosan. The results demonstrated that gelatin and chitosan caused a significant enhancement in the bioactivity of the scaffold, which confirmed by MTT assay and improved the cell spreading and proliferation of neural cell on the scaffolds.
Conclusion: Based on the experimental results, the PCL/chitosan/PPy conductive substrate could be used as a potential scaffold for clinical research in the field of neural regeneration and healing.
- Electrospinning
- Nerve graft
- Neural tissue engineering
- Nanofibrous
- Surface modification.
How to Cite
References
Reference:
Willerth SM, Sakiyama-Elbert SE. Approaches to neural tissue engineering using scaffolds for drug delivery. Advanced drug delivery reviews. 2007;59(4):325-38.
Geuna S, Gnavi S, Perroteau I, Tos P, Battiston B. Tissue engineering and peripheral nerve reconstruction: An overview. Int Rev Neurobiol. 2013;108:35-57.
Schmidt CE, Leach JB. Neural tissue engineering: strategies for repair and regeneration. Annual review of biomedical engineering. 2003;5(1):293-347.
Tam RY, Fuehrmann T, Mitrousis N, Shoichet MS. Regenerative therapies for central nervous system diseases: a biomaterials approach. Neuropsychopharmacology. 2014;39(1):169-88.
Entekhabi E, Nazarpak MH, Moztarzadeh F, Sadeghi A. Design and manufacture of neural tissue engineering scaffolds using hyaluronic acid and polycaprolactone nanofibers with controlled porosity. Materials Science and Engineering: C. 2016;69:380-7.
Ai J, Kiasat-Dolatabadi A, Ebrahimi-Barough S, Ai A, Lotfibakhshaiesh N, Norouzi-Javidan A, et al. Polymeric scaffolds in neural tissue engineering: a review. Archives of Neuroscience. 2014;1(1):15-20.
Yu X, Bellamkonda RV. Tissue-engineered scaffolds are effective alternatives to autografts for bridging peripheral nerve gaps. Tissue engineering. 2003;9(3):421-30.
Subramanian A, Krishnan UM, Sethuraman S. Development of biomaterial scaffold for nerve tissue engineering: Biomaterial mediated neural regeneration. Journal of biomedical science. 2009;16(1):108.
Cui W, Zhou Y, Chang J. Electrospun nanofibrous materials for tissue engineering and drug delivery. Science and Technology of Advanced Materials. 2016.
Jiang X, Mi R, Hoke A, Chew SY. Nanofibrous nerve conduit‐enhanced peripheral nerve regeneration. Journal of tissue engineering and regenerative medicine. 2014;8(5):377-85.
Yang F, Murugan R, Wang S, Ramakrishna S. Electrospinning of nano/micro scale poly (L-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials. 2005;26(15):2603-10.
Prabhakaran MP, Venugopal JR, Chyan TT, Hai LB, Chan CK, Lim AY, et al. Electrospun biocomposite nanofibrous scaffolds for neural tissue engineering. Tissue Engineering Part A. 2008;14(11):1787-97.
Cao H, Liu T, Chew SY. The application of nanofibrous scaffolds in neural tissue engineering. Advanced drug delivery reviews. 2009;61(12):1055-64.
Sivolella S, Brunello G, Ferrarese N, Della Puppa A, D'Avella D, Bressan E, et al. Nanostructured guidance for peripheral nerve injuries: a review with a perspective in the oral and maxillofacial area. International journal of molecular sciences. 2014;15(2):3088-117.
Yang F, Murugan R, Ramakrishna S, Wang X, Ma Y-X, Wang S. Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering. Biomaterials. 2004;25(10):1891-900.
Hoffman-Kim D, Mitchel JA, Bellamkonda RV. Topography, cell response, and nerve regeneration. Annual review of biomedical engineering. 2010;12:203-31.
Woodruff MA, Hutmacher DW. The return of a forgotten polymer—polycaprolactone in the 21st century. Progress in Polymer Science. 2010;35(10):1217-56.
Garcia Cruz DM, Coutinho DF, Costa Martinez E, Mano JF, Gómez Ribelles JL, Salmerón Sánchez M. Blending polysaccharides with biodegradable polymers. II. Structure and biological response of chitosan/polycaprolactone blends. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2008;87(2):544-54.
Eyrich D, Wiese H, Maier G, Skodacek D, Appel B, Sarhan H, et al. In vitro and in vivo cartilage engineering using a combination of chondrocyte-seeded long-term stable fibrin gels and polycaprolactone-based polyurethane scaffolds. Tissue engineering. 2007;13(9):2207-18.
Jiang Y-C, Jiang L, Huang A, Wang X-F, Li Q, Turng L-S. Electrospun polycaprolactone/gelatin composites with enhanced cell–matrix interactions as blood vessel endothelial layer scaffolds. Materials Science and Engineering: C. 2017;71:901-8.
Baniasadi H, SA AR, Mashayekhan S. Fabrication and characterization of conductive chitosan/gelatin-based scaffolds for nerve tissue engineering. International journal of biological macromolecules. 2015;74:360-6.
Kim I-Y, Seo S-J, Moon H-S, Yoo M-K, Park I-Y, Kim B-C, et al. Chitosan and its derivatives for tissue engineering applications. Biotechnology advances. 2008;26(1):1-21.
Wrobel S, Serra SC, Ribeiro-Samy S, Sousa N, Heimann C, Barwig C, et al. In vitro evaluation of cell-seeded chitosan films for peripheral nerve tissue engineering. Tissue Engineering Part A. 2014;20(17-18):2339-49.
Dang JM, Leong KW. Natural polymers for gene delivery and tissue engineering. Advanced drug delivery reviews. 2006;58(4):487-99.
Sionkowska A. Current research on the blends of natural and synthetic polymers as new biomaterials. Progress in polymer science. 2011;36(9):1254-76.
Gautam S, Chou C-F, Dinda AK, Potdar PD, Mishra NC. Surface modification of nanofibrous polycaprolactone/gelatin composite scaffold by collagen type I grafting for skin tissue engineering. Materials Science and Engineering: C. 2014;34:402-9.
Martins A, Pinho ED, Faria S, Pashkuleva I, Marques AP, Reis RL, et al. Surface modification of electrospun polycaprolactone nanofiber meshes by plasma treatment to enhance biological performance. small. 2009;5(10):1195-206.
Yan D, Jones J, Yuan X, Xu X, Sheng J, Lee JM, et al. Plasma treatment of electrospun PCL random nanofiber meshes (NFMs) for biological property improvement. Journal of Biomedical Materials Research Part A. 2013;101(4):963-72.
Lee JY, Bashur CA, Goldstein AS, Schmidt CE. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials. 2009;30(26):4325-35.
Murphy CM, Haugh MG, O'Brien FJ. The effect of mean pore size on cell attachment, proliferation and migration in collagen–glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials. 2010;31(3):461-6.
Collins MN, Birkinshaw C. Hyaluronic acid based scaffolds for tissue engineering—A review. Carbohydrate polymers. 2013;92(2):1262-79.
Hollister SJ. Porous scaffold design for tissue engineering. Nature materials. 2005;4(7):518.
Bružauskaitė I, Bironaitė D, Bagdonas E, Bernotienė E. Scaffolds and cells for tissue regeneration: different scaffold pore sizes—different cell effects. Cytotechnology. 2016;68(3):355-69.
Christopherson GT, Song H, Mao H-Q. The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. Biomaterials. 2009;30(4):556-64.
- Abstract Viewed: 294 times
- PDF Downloaded: 375 times