International Clinical Neuroscience Journal

Vol. 3 No. 3 (2016)

Electrospun Poly Caprolactone-Carbon Nanotube Scaffold for Nerve Regeneration in Dental Tissue Engineering

International Clinical Neuroscience Journal, Vol. 3 No. 3 (2016), 7 Azar 2016 , Page 144-149
https://doi.org/10.22037/icnj.v3i3.14420

Abstract

Regeneration and engineering of functional new tissues containing the neural network have great importance. Progression of neural network into the dental tissue has a crucial role in dental tissue regeneration. In the present study polymer-ceramic blended scaffolds containing different weight percentages of carbon nanotube in poly caprolactone nanofiber matrix were fabricated. Morphological, mechanical and electrical properties of the prepared scaffolds have been characterized. Results showed that the sample containing 5 weight % of carbon nanotube had the smallest mean fiber diameter (50 - 300 nm) and the highest mechanical behavior. Also, its electrical conductivity was suitable to be used in nerve tissue scaffolds. The static culture of the Schwann cells on the prepared scaffolds indicated that increasing weight percentage of carbon nanotube into the polycaprolactone matrix up to the 5 wt. % enhanced cell viability.

Keywords:
  • Nerve Tissue Engineering
  • Carbon Nano Tube
  • Polycaprolactone
  • Nano Fiber
  • Schwann Cells
  • Characterization

How to Cite

1.
Ketabi MA, Shanavazi M, Fekrazad R, Tondnevis F, Keshvari H, Raz M, Sadeghi A, Bajelani K, shahrousvand M, Zalli AR, Mohseni G, Abolhasani MM. Electrospun Poly Caprolactone-Carbon Nanotube Scaffold for Nerve Regeneration in Dental Tissue Engineering. Int Clin Neurosci J [Internet]. 2016 Dec. 7 [cited 2023 Dec. 9];3(3):144-9. Available from: https://journals.sbmu.ac.ir/neuroscience/article/view/14420

References

Kumar PA, Hassan KM. Cross-face nerve graft with free-muscle transfer for reanimation of the paralyzed face: a comparative study of the single-stage and two-stage procedures. Plast Reconstr Surg. 2002 Feb;109(2):451-62; discussion 463-4.

Bae YC, Zuker RM, Manktelow RT, Wade S. A comparison of commissure excursion following gracilis muscle transplantation for facial paralysis using a cross-face nerve graft versus the motor nerve to the masseter nerve. Plast Reconstr Surg. 2006 Jun;117(7):2407-13.

Koshima I, Tsuda K, Hamanaka T, Moriguchi T. One-stage reconstruction of established facial paralysis using a rectus abdominis muscle transfer. Plast Reconstr Surg. 1997 Jan;99(1):234-8.

Harii K, Asato H, Yoshimura K, Sugawara Y, Nakatsuka T, Ueda K. One-stage transfer of the latissimus dorsi muscle for reanimation of a paralyzed face: a new alternative. Plast Reconstr Surg. 1998 Sep;102(4):941-51.

Hayashi A, Maruyama Y. Neurovascularized free short head of the biceps femoris muscle transfer for one-stage reanimation of facial paralysis. Plast Reconstr Surg. 2004; 115, 394.

Suzuki Y, Tanihara M, Ohnishi K, Suzuki K, Endo K, Nishimura Y. Cat peripheral nerve regeneration across 50 mm gap repaired with a novel nerve guide composed of freeze-dried alginate gel. Neurosci Lett. 1999 Jan 8;259(2):75-8.

Matsumoto K, Ohnishi K, Kiyotani T, Sekine T, Ueda H, Nakamura T, et al. Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: a histological and electrophysiological evaluation of regenerated nerves. Brain Res. 2000 Jun 23;868(2):315-28.

Lindvall O, Kokaia Z. Recovery and rehabilitation in stroke: Stem cells. Stroke 2004;35(suppl 1):2691–2694.

Keirstead HS, Nistor G, Bernal G, Totoiu M, Cloutier F, Sharp K, et al. Human embryonic stem cell-derived oligodendrocyte progenitor cell transplants remyelinate and restore locomotion after spinal cord injury. J Neurosci. 2005 May 11;25(19):4694-705.

Arthur A, Rychkov G, Shi S, Koblar SA, Gronthos S. Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem Cells. 2008 Jul;26(7):1787-95.

Sasaki R, Aoki S, Yamato M, Uchiyama H, Wada K, Okano T, et al. Tubulation with dental pulp cells promotes facial nerve regeneration in rats. Tissue Eng Part A. 2008 Jul;14(7):1141-7.

Deitzel JM, Kleinmeyer J, Harris D, Tan NCB. Polymer 2001;42:261–72.

Subbiah T, Bhat GS, Tock RW, Parameswaran S, Ramkumar SS. Electrospinning of nanofibers. J Appl Polym Sci. 2005; 96: 557–569.

Eitan A, Jiang K, Dukes D, Andrews R, Schadler LS. Surface modification of multiwalled carbon nanotubes: toward the tailoring of the interface in polymer composites. Chem Mater 2003;15(16):3198–201.

We, Guobao, Peter X Ma. Structure and properties of Nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering.Biomaterials. 2004; 25(19): 4749-4757.

Ioannis SK, Grapenson S, Jakob A. "Conductive polypyrrole Nanofibers via electrospinning: electrical and morphological properties. Polymer. 2006: 47(5):1597-1603.

Yang X, Yang F, Walboomers XF, Bian Z, Fan M, Jansen JA. The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. J Biomed Mater Res A. 2010 Apr;93(1):247-57.

Haastert K, Seef P, Stein VM, Tipold A, Grothe C. A new cell culture protocol for enrichment and genetic modification of adult canine Schwann cells suitable for peripheral nerve tissue engineering. Res Vet Sci. 2009 Aug;87(1):140-2.

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