Evaluation of Van A-B genes frequency in Vancomycin Resistant Enterococci spp., isolated from clinical samples of Imam Hossein Teaching Hospital in Tehran.: A molecular study of vancomycin-resistant-enterococci.
Novelty in Biomedicine,
Vol. 9 No. 3 (2021),
13 August 2021
,
Page 111-117
https://doi.org/10.22037/nbm.v9i3.33042
Abstract
Abstract
Background: Antibiotic resistance is an important cause of treatment failure and re-infection in enterococci. In this study, the frequency of phenotype and genotype of Van A-B genes in Vancomycin resistant enterococcus isolated from a clinical sample of Imam Hossein Hospitals in Tehran was determined.Materials and Methods: In this descriptive cross-sectional study in 2018, a total of 76 vancomycin-resistant enterococci in Imam Hossein Hospitals in Tehran were evaluated, including those from blood, urine, sputum, and wound. The frequency of phenotype and genotype of Van A-B genes in them was determined by MIC Epsilometer test and Multiplex Real-time PCR.
Results: The 160 isolates of enterococci collected from different hospital wards revealed that 76 (47%) enterococci were resistant by applying MIC E-test; interestingly, all VRE isolated showed high-level vancomycin resistance. The Real-time-PCR assay demonstrated vanA gene in 76 (100%) VRE isolates. Considering to controls no van B gene was detected in this assay. Based on bacterial phenotype tests, the results showed that 82% and 18% of the isolates were E. faecium and E. faecalis, respectively.
Conclusion: Totally, it may be concluded that Van A gene is more accompanied by high-level Vancomycin Teicoplanin resistance in common enterococci species. The frequency of Vancomycin resistance entrocooci is increasing especially among ICU admitted patients. For effective treatment MIC test and Van A-B genotyping seem to be essential.
- van A, van B, Vancomicin resistance entrococci, Real-time PCR
How to Cite
References
Paul A, John A, Dennis G. Nosocomial Infection with vancomycin dependent Enterococci. 2004.
Nikooei M, Meidani M, Khorvash F, Karimi M, Parsaei P Evaluation of the frequency of phenotype and genotype of Van A and Van B genes in Vancomycin resistant enterococcus isolated from clinical sample of Alzahra Hospitals in Isfahan. J Shahrekord Univ Med Sci. 2014;16(3):61-9.
Hosseini F,Karegar M. staudy of frequency of Vana,Vanb C1/C2 genes in vancomycin resistance Entrococci strains Isolated from hospitalized Patien in sout of Farz provience.JSSU.2017;24(12):936-71.
Vahabi A,Hassani A,Nahaei A. PCR assay for detection of vanA and van B genes from intestinal carriers of vancomycin-resistance-entrococci in three edicational hospital of tabriz university of medical science.Iranian Journal of medical micrbiology 58-65, 2010.
Masoumi Zavaryani S, Mirnejd R, Zareh S, Piranfar V, Bagheri Bejestani O. Identification of Enterococci faecalis&E. faeacium pathogens via Tehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016. JMJ. 2016;14(3):52-9.
Mohammadi SF, Tabarraei B, Sadeghifard N, et al. resistance rate of Enterococci faecalis and E. faeacium and Van A/B gene identification by PCR in Vancomycin resistant species in Ilam and Kermanshah. Ilam Univ Med J. 2011;19(2):1-8.
Shahraki S, Rabi Nezhad Mousavi M, Dahmarde B, Atashgah M. Molecular evaluation of vanA and vanB genes of enterococci isolates resistant to Vancomycin and Teicoplanin. J Gorgan Univ Med Sci. 2017;19(2):84-90.
Arbabi L, Boustanshenas M, Adabi M, Fathizadeh S, Rasouli koohi S, Afshar M, et al . Isolation and Antibiotic Susceptibility Pattern among Vancomycin Resistant Enterococci Isolated from Clinical Samples of Different Parts of Rasoul-E-Akram Hospital. J Ardabil Univ Med Sci. 2016;15(4):404-13
Najafi Mosleh M, Nasaj M, Rahimi F, Arabestani M R. Distribution Rates and Antibiotic Resistance Pattern of Enterococcus Spp. Isolated from Clinical Specimens of Hospitals in Hamedan. J Mazandaran Univ Med Sci. 2014;24(117):92-102.
McPherson RA, Pincus MR. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23th ed. St. Louis, Missouri: Elsevier; 2017. P.1159-62.
P. Asadollahi, Sh. Razavi, Kh. Asadollahi, M. R. Pourshafie and M. Talebi Rise of antibiotic resistance in clinical enterococcal isolates during 2001–2016 in Iran: a review.
M Emaneini et al Pervance of vancomycin resistence entrococcus in IRAN: A systematc review and Meta-Analysis Eur J clin Microbal Infec 2016.
A Moghimbeigi,M Moghimbeygi, M dousti, F kiani ,F Sayehmiri ,N sadeghifard et al. Pervalence of vancomycin resistence entrococcus in IRAN : A systematc review and Meta-Analysis. Adolesc Health Med Ther. 2018;9:177-88.
Taneja N, Rani P, Emmanuel R, Sharma M. Significance of Vancomycin resistant enterococci from urinary specimens at a tertiary care centre in northern India. Indian Journal of Medical Research. 2004;119(2):72.
Frakking F, Bril W, Sinnige J, van’t Klooster J, de Jong B, van Hannen E, et al. Recommendations for the successful control of a large outbreak of vancomycin-resistant Enterococcus faecium (VRE) in a non-endemic hospital setting. Journal of Hospital Infection. 2018.
Ndubuisi J, Olonitola O, Olayinka A, Jatau E, Iregbu K. Prevalence and antibiotics susceptibility profile of Enterococcus spp. Isolated from some hospitals in Abuja, Nigeria. African Journal of Clinical and Experimental Microbiology. 2017;18(3):154-8.
Bashir A, Attie O, Sullivan M, Sebra R, Singh KV, Altman D, et al. Genomic confirmation of vancomycin-resistant Enterococcus transmission from deceased donor to liver transplant recipient. PloS one. 2017;12(3):e0170449.
- Abstract Viewed: 130 times
- pdf Downloaded: 111 times