Cloning and Expression of Luteinizing Hormone Subunits in Chinese Hamster Ovary Cell Line
Novelty in Biomedicine,
Vol. 4 No. 4 (2016),
30 October 2016
AbstractBackground: Luteinizing hormone (LH) was secreted by the stimulating cells of the testes and ovaries in the anterior pituitary gland. The application of this hormone is in the treatment of men and women with infertility and amenorrhea respectively.
Materials and Methods: In the present study the alpha and beta subunits of human LH gene were cloned into the pEGFP-N1 expression vector and produced the recombinant LH hormone in Chinese hamster ovary (CHO) eukaryotic system.
Results: Alpha and beta subunits of LH hormone were cloned between NheI and BamHI cut sites of pEGFP_N1 expression plasmid and confirmed by PCR. Hormone expression was evaluated in CHO cell line by Western blotting using the specific antibody.
Conclusion: Alpha and beta subunits of LH hormone were expressed in CHO cell line perfectly.
- Recombinant Hormone
How to Cite
Sabooryaraghi A, Ghods R, Shokri F. Production and characterization of a murine monoclonal antibody recognizing a conformational epitope on hCG. J Reproduc & Infert. 2000;1(4):44-54.
Louvet J, Harman S, Ross G. Effects of human chorionic gonadotropin, human interstitial cell stimulating hormone and human follicle-stimulating hormone on ovarian weights in estrogen-primed hypophysectomized immature female rats. Endocrinol. 1975;96(5):1179–86.
Jiang X, Dias JA, He X. Structural biology of glycoprotein hormones and their receptors: Insights to signaling. Mol Cell Endocrinol. 2013;382(1):424–51.
Bowen R. Gonadotropins: Luteinizing and Follicle Stimulating Hormones. Colorado State University. Available from: www.google.com. Accessed. 2004.
Carlsson GH, Nicholls P, Svistunenko D, Berglund GI, Hajdu J. "Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide". Biochem. 2005;44(2):635–42.
Gajhede M, Schuller DJ, Henriksen A, Smith AT, Poulos TL. Crystal structure of horseradish peroxidase C at 2.15 A resolution. Nat Struct Biol. 1997;4(12):1032–8.
Ventura SJ, Martin JA, Curtin SC, Mathews TJ, Park MM. Births: final data for 1998. Natl Vital Stat Rep. 2000;48(3):1–100.
Ventura SJ, Mosher WD, Curtin SC, Abma JC, Henshaw S. Trends in pregnancy rates for the United States, 1976–97: an update. Natl Vital Stat Rep. 2001;49(4):1–9.
Weijer V, Mulders BH, Bos JW, Verhaert ES, Van Den Hooven P. Compositional analyses of a human menopausal gonadotrophin preparation extracted from urine (menotropin). Identification of some of its major impurities. Reproduct Biomed. 2003;7(5):547–57.
Razi M, Mohseni F, Firouzabadi R, Janati S, Yari N, Etebary S. Recombinant LH for assisted reproductive technology treatment. Iran J Reprod Med. 2014;12(2):111-6.
Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nature Biotech. 2004;22(11):1393-8.
Li X, Zhao X, Fang Y, Jiang X, Duong T, Fan C. Generation of destabilized green fluorescent protein as a transcription reporter. J of Biological Chem. 1998;273(52):34970.
Jayapal KP, Wlaschin KF, Hu W, Yap MGS. Recombinant protein therapeutics from CHO cells-20 years and counting. Chem Engin Prog. 2007;103(10):40.
Zmora N1, Kazeto Y, Kumar RS, Schulz RW, Trant JM. Production of recombinant channel catfish (Ictalurus punctatus) FSH and LH in S2 Drosophila cell line and an indication of their different actions. J Endocrinol. 2007;194(2):407-16.
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