First Report of Vermamoeba vermiformis in the Island of El Hierro, Canary Islands, Spain
Novelty in Biomedicine,
Vol. 4 No. 4 (2016),
30 October 2016
AbstractBackground: Free-living amoebae (FLA) are group of protozoa distributed worldwide in many habitats mainly water and soil related sources. Some members of FLA are able to act as opportunistic pathogens and are environmental carriers of other pathogenic agents such as bacteria and viruses. Vermamoeba vermiformis is a highly abundant FLA species in water bodies and has recently gained environmental importance as it acts as a vehicle of many pathogenic bacteria such as Legionella pneumophila.
Cases Report: In this study, water samples were collected from the island of El Hierro, Canary Islands, Spain during 2015. El Hierro island was designated by UNESCO as a biosphere reserve and it is currently the less populated of the Canary Islands. The water samples were culture on 2 % Non-Nutrient Agar (NNA) plates covered with a thin layer of heat killed E. coli and checked daily for the presence of FLA. After a week, V. vermiformis amoebae were observed in the plates incubated at room temperature and 37 ºC. Molecular characterization was carried out by amplifying the 18S rDNA gene and DNA sequencing, confirming that the isolated strain belonged to Vermamoeba vermiformis species.
Conclusion: To the best of our knowledge, this is the first report of Vermamoeba vermiformis isolation in the island of El Hierro and the second report of this species in the Canary Islands.
- Vermamoeba vermiformis
- Canary Islands
How to Cite
Thomas V, Loret JF, Jousset M, Greub G. Biodiversity of amoebae and amoebae-resisting bacteria in a drinking water treatment plant. Environ Microbiol. 2008;10:2728-45
Pagnier I, Valles C, Raoult D, La Scola B. Isolation of Vermamoeba vermiformis and associated bacteria in hospital water. Microb Pathog. 2015;80:14-20.
Fouque E, Yefimova M, Trouilhé MC, Quellard N, Fernandez B, Rodier MH, Thomas V, Humeau P, Héchard Y. Morphological Study of the Encystment and Excystment of Vermamoeba vermiformis Revealed Original Traits. J Eukaryot Microbiol. 2015; 62(3):327-37.
Lorenzo-Morales J, Coronado-Álvarez N, Martínez-Carretero E, Maciver SK, Valladares B. Detection of four adenovirus serotypes within water-isolated strains of Acanthamoeba in the Canary Islands, Spain. Am. J. Tropical Med. Hygiene. 2007;77(4):753-6.
Lorenzo-Morales J, Khan NA, Walochnik J An update on Acanthamoeba keratitis: diagnosis, pathogenesis and treatment. Parasite. 2015;22:10
Richards AM, Von Dwingelo JE, Price CT, Abu Kwaik Y. Cellular microbiology and molecular ecology of Legionella-amoeba interaction. Virulence. 2013;4(4):307-14.
Thomas V, McDonnell G, Denyer SP, Maillard JY. Free-living amoebae and their intracellular pathogenic microorganisms: risks for water quality. FEMS Microbiol Rev. 2010;34:231-59.
Fouque E, Trouilhé MC, Thomas V, Humeau P, Héchard Y. Encystment of Vermamoeba (Hartmannella) vermiformis: Effects of environmental conditions and cell concentration. Exp Parasitol. 2014;145 Suppl:S62-8.
Solgi R, Niyyati M, Haghighi A, Mojarad EN. Occurrence of Thermotolerant Hartmannella vermiformis and Naegleria Spp. in Hot Springs of Ardebil Province, Northwest Iran. Iran J Parasitol. 2012;7(2):47-52.
Bonilla-Lemus P, Caballero Villegas AS, Carmona Jiménez J, Lugo Vázquez A. Occurrence of free-living amoebae in streams of the Mexico Basin. Exp Parasitol. 2014; 145 Suppl:S28-33.
Reyes-Batlle M, Niyyati M, Martín-Navarro CM, López-Arencibia A, Valladares B, Martínez-Carretero E, Piñero JE, Lorenzo-Morales J. Unusual Vermamoeba vermiformis Strain Isolated from Snow in Mount Teide, Tenerife, Canary Islands, Spain. Novel Biomed. 2015;3(4):189-92.
Reyes-Batlle M, Todd CD, Martín-Navarro CM, López-Arencibia A, Cabello-Vilchez AM, González AC, et al. Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain. Parasitol. Res. 2014;113(4):1383-8.
Smirnov AV, Chao E, Nassonova ES, Cavalier-Smith T. A revised classification of naked lobose amoebae (Amoebozoa: lobosa). Protist. 2011;162:545-70.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution. 2011;28:2731-9.
Page EC. A New Key to Freshwater and Soil Gymnamoebae. Freshwater Biological Association, Cumbria, England, UK. 1988.
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