Expression Profiles of P53, Caspase-3 And Bcl-2 in Patients Undergoing Congenital Heart Corrective surgery: Combined Effects Of Anesthesia And Surgery
Journal of Cellular & Molecular Anesthesia,
Vol. 6 No. 4 (2021),
21 January 2022
,
Page 286-293
https://doi.org/10.22037/jcma.v6i4.36022
Abstract
Background: Apoptosis is a physiological programmed cell death necessary for development and cellular homeostasis. Dysregulation of apoptosis pathways leads to a number of diseases such as cancer, autoimmune and immunodeficiency diseases, and neuro-degenerative disorders.
Patients and methods: The current study included 56 patients (29 males and 27 females), undergoing corrective heart surgery operations, categorized into 3 groups: group A included 23 patients with atrial septal defect (ASD); group B included 15 patients with ventricular septal defect (VSD); and group C included 15 patients with fallot tetralogy. Biochemical assays of apoptotic (P53 and caspase 3) and antiapoptotic markers (Bcl-2) using colorimetric and ELISA assay kits were performed to all included patients twice, preoperative and 24 hours postoperative.
Results: No statistical significant difference was found in the preoperative levels of Bcl-2, Caspase-3 and P53 between the three groups. While significant difference was found when comparing the preoperative and postoperative levels of the previous markers in the same group and between groups (p˂0.05 for all).
Conclusion: Both apoptotic and antiapoptotic pathways are activated during congenital heart corrective surgeries. Degree of markers increase is directly related to duration of anesthesia exposure.
Key words: P53; caspase-3; Bcl-2; congenital heart corrective surgery; Apotosis.
- P53
- Caspase-3
- Bcl-2
- Congenital Heart Surgery
- Apoptosis
How to Cite
References
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