Effects of mesenchymal stem cells conditioned medium on behavioral aspects of inflammatory arthritic pain induced by CFA adjuvant
Journal of Cellular & Molecular Anesthesia,
Vol. 1 No. 2 (2016),
Background: Rheumatoid arthritis is a type of inflammatory pain and is an autoimmune and chronic inflammatory disease which can lead to hyperalgesia, edema and decreased motor activity in affected area. Mesenchymal stem cells conditioned medium (MSC-CM) has anti-inflammatory mediators which can regulate the immune responses, alleviate inflammatory symptoms and has a paracrine effects too. The aim of this study was to evaluate the effects of mesenchymal stem cells conditioned medium on behavioral aspects of inflammatory arthritic pain which induced by CFA adjuvant.
Materials and Methods: Complete Freund’s adjuvant (CFA)-induced arthritis (AA) was caused by single subcutaneous injection of CFA into the rats hind paw on day zero. MSC-CM was administered daily and intraperitoneal during the 21 days of the study after CFA injection. Hyperalgesia and edema were assessed on days 0, 7, 14 and 21 of the study respectively with radian heat and plethysmometer instrument.
Results: The results of this study indicated the significant roles of MSC-CM in betterment of inflammatory symptoms such as hyperalgesia and edema during different stages of inflammation caused by CFA. The continuing injection of MSC-CM could reduce the inflammatory symptoms.
Conclusion: Long term treatment by MSC-CM can alleviate hyperalgesia and edema and decrease those to the level of the time before induction of inflammation.
How to Cite
Sommer C, Kress M. Recent findings on how proinflammatory cytokines cause pain: peripheral mechanisms in inflammatory and neuropathic hyperalgesia. Neurosci Lett. 2004;361(1):184-7.
Kojima M, Kojima T, Suzuki S, Takahashi N, Funahashi K, Kato D, et al. Alexithymia, depression, inflammation, and pain in patients with rheumatoid arthritis. Arthritis Care Res. 2014;66(5):679-86.
Elenkov IJ, Wilder RL, Chrousos GP, Vizi ES. The sympathetic nerve—an integrative interface between two supersystems: the brain and the immune system. Pharmacol rev. 2000;52(4):595-638.
Troullos ES, Hargreaves KM, Butler DP, Dionne RA. Comparison of nonsteroidal anti-inflammatory drugs, ibuprofen and flurbiprofen, with methylprednisolone and placebo for acute pain, swelling, and trismus. J Oral Maxillofac Surg. 1990;48(9):945-52.
Manning DC, Raja SN, Meyer RA, Campbell JN. Pain and hyperalgesia after intradermal injection of bradykinin in humans. Clin Pharmacol Ther. 1991;50(6):721-9.
Zaringhalam J, Manaheji H, Mghsoodi N, Farokhi B, Mirzaiee V. Spinal µ‐opioid receptor expression and hyperalgesia with dexamethasone in chronic adjuvant‐induced arthritis in rats. Clin Exp Pharmacol Physiol. 2008;35(11):1309-15.
Fessler E, Dijkgraaf FE, Felipe De Sousa EM, Medema JP. Cancer stem cell dynamics in tumor progression and metastasis: is the microenvironment to blame? Cancer Lett. 2013;341(1):97-104.
Platas J, Guillén MI, del Caz MDP, Gomar F, Mirabet V, Alcaraz MJ. Conditioned media from adipose-tissue-derived mesenchymal stem cells downregulate degradative mediators induced by interleukin-1β in osteoarthritic chondrocytes. Mediators Inflamm. 2013;2013.
Ivanova-Todorova E, Bochev I, Dimitrov R, Belemezova K, Mourdjeva M, Kyurkchiev S, et al. Conditioned Medium from Adipose Tissue-Derived Mesenchymal Stem Cells Induces CD4. Biomed Res Int. 2012;2012.
Jorgensen C, Noel D. Mesenchymal stem cells in osteoarticular diseases: an update. Int J Mo1 Cell Med Winter. 2012;1(1):2.
Zaringhalam J, Akhtari Z, Eidi A, Ruhani AH, Tekieh E. Relationship between serum IL10 level and p38MAPK enzyme activity on behavioral and cellular aspects of variation of hyperalgesia during different stages of arthritis in rats. Inflammopharmacology. 2014;22(1):37-44.
Zaringhalam J, Hormozi A, Tekieh E, Razavi J, Khanmohammad R, Golabi S. Serum IL-10 involved in morphine tolerance development during adjuvant-induced arthritis. J Physiol Biochem. 2014;70(2):497-507.
Aali E, Mirzamohammadi S, Ghaznavi H, Madjd Z, Larijani B, Rayegan S, et al. A comparative study of mesenchymal stem cell transplantation with its paracrine effect on control of hyperglycemia in type 1 diabetic rats. J Diabetes Metab Disord. 2014;13(1):1.
Rezazadeh S, Zaringhalam J, Manaheji H, Kebryaeezadeh A. Anti-inflammatory and anti–hyperalgesic activities of Stachys athorecalyx extracts on CFA-induced inflammation. J Med Plant Res. 2009;3(5):368-76.
Zaringhalam J, Tekieh E, Manaheji H, Akhtari Z. Cellular events during arthritis-induced hyperalgesia are mediated by Interleukin-6 and p38 MAPK and their effects on the expression of spinal mu-opioid receptors. Rheumatol Int. 2013;33(9):2291-9.
LaBuda CJ, Fuchs PN. A behavioral test paradigm to measure the aversive quality of inflammatory and neuropathic pain in rats. Exp Neurol. 2000;163(2):490-4.
Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain. 1988;32(1):77-88.
Iadarola MJ, Brady LS, Draisci G, Dubner R. Enhancement of dynorphin gene expression in spinal cord following experimental inflammation: stimulus specificity, behavioral parameters and opioid receptor binding. Pain. 1988;35(3):313-26.
Cicala C, Ianaro A, Fiorucci S, Calignano A, Bucci M, Gerli R, et al. NO‐naproxen modulates inflammation, nociception and downregulates T cell response in rat Freund's adjuvant arthritis. Br J Pharmacol. 2000;130(6):1399-405.
Nagakura Y, Okada M, Kohara A, Kiso T, Toya T, Iwai A, et al. Allodynia and hyperalgesia in adjuvant-induced arthritic rats: time course of progression and efficacy of analgesics. J Pharmacol Exp Ther. 2003;306(2):490-7.
Hammond D. Persistent inflammatory nociception and hyperalgesia: implications for opioid actions in the brainstem and spinal cord. Hyperalgesia: Molecular Mechanisms and Clinical Implications (Brune K and Handwerker HO eds) pp. 2004:291-309.
Yew T-L, Hung Y-T, Li H-Y, Chen H-W, Chen L-L, Tsai K-S, et al. Enhancement of wound healing by human multipotent stromal cell conditioned medium: the paracrine factors and p38 MAPK activation. Cell Transplant. 2011;20(5):693-706.
Maumus M, Jorgensen C, Noël D. Mesenchymal stem cells in regenerative medicine applied to rheumatic diseases: role of secretome and exosomes. Biochimie. 2013;95(12):2229-34.
- Abstract Viewed: 597 times
- PDF Downloaded: 191 times