Expression and enzyme activity of MnSOD and catalase in peripheral blood mononuclear cells isolated from multiple sclerosis patients
Archives of Medical Laboratory Sciences,
Vol. 1 No. 1 (2015),
21 May 2015
https://doi.org/10.22037/amls.v1i1.8964
Abstract
Background: It is evident that oxidative stress plays a crucial role in etiology of multiple sclerosis (MS). Dysregulation of antioxidant enzymes have been implicated in demylination and neuronal loss in MS. The aim of this study was to evaluate mRNA expression and activity of manganese superoxide dismutase (MnSOD), and catalase in peripheral blood mononuclear cells (PBMCs) from patients with relapsing-remitting multiple sclerosis (RRMS) and healthy controls.
Materials and Methods: We recruited 20 RRMS patients and 20 age-and sex-matched healthy subjects. PBMCs were isolated, RNA was extracted and real time-PCR was used to evaluate mRNA expression of MnSOD and catalase. Enzyme activity of MnSOD and catalase were measured using colorimetric assays.
Results: We found a significant increase in mRNA expression and activity of catalase in PBMCs from patients compared with controls, which was accompanied by reduced activity and expression of MnSOD in MS patients.
Conclusion: It appears that impaired antioxidant enzymes in term of high activity of catalase and decreased activity of MnSOD are involved in MS pathogenesis, however further studies are needed to establish this concept.
- multiple sclerosis
- enzyme activity
- Real Time-PCR
How to Cite
References
Marrie RA. Environmental risk factors in multiple sclerosis aetiology. Lancet Neurol. 2004;3:709-18.
Friese MA, Schattling B, Fugger L. Mechanisms of neurodegeneration and axonal dysfunction in multiple sclerosis. Nat Rev Neurol. 2014;10:225-38.
Emamgholipour S, Eshaghi SM, Hossein-nezhad A, Mirzaei K, Maghbooli Z, et al. Adipocytokine profile, cytokine levels and foxp3 expression in multiple sclerosis: a possible link to susceptibility and clinical course of disease. PLoS One. 2013;8:e76555.
Gironi M, Borgiani B, Mariani E, Cursano C, Mendozzi L, et al. (2014) Oxidative stress is differentially present in multiple sclerosis courses, early evident, and unrelated to treatment. J Immunol Res. 2014;961863.
Goertsches R, Zettl UK. MS therapy research applying genome-wide RNA profiling of peripheral blood. Int MS J. 2007;14: 98-107.
Horssen J, Witte ME, Schreibelt G, Vries HE. Radical changes in multiple sclerosis pathogenesis. Biochim Biophys Acta. 2011;18(12):141-50.
Smith KJ, Kapoor R, Felts PA. Demyelination: the role of reactive oxygen and nitrogen species. Brain Pathol. 1999;9:69-92.
Schreibelt G, Horssen J, Rossum S, Dijkstra CD, Drukarch B, et al. Therapeutic potential and biological role of endogenous antioxidant enzymes in multiple sclerosis pathology. Brain Res Rev. 2007;56:322-30.
Horssen J, Schreibelt G, Drexhage J, Hazes T, Dijkstra CD, et al. Severe oxidative damage in multiple sclerosis lesions coincides with enhanced antioxidant enzyme expression. Free Radic Biol Med. 2008;45:1729-37.
Horssen J, Drexhage JA, Flor T, Gerritsen W, Valk P, et al. Nrf2 and DJ1 are consistently upregulated in inflammatory multiple sclerosis lesions. Free Radic Biol Med. 2010;49:1283-9.
Mirshafiey A, Mohsenzadegan M. Antioxidant therapy in multiple sclerosis. Immunopharmacol Immunotoxicol. 2009;31:13-29.
Hybertson BM, Gao B, Bose SK, McCord JM. Oxidative stress in health and disease: the therapeutic potential of Nrf2 activation. Mol Aspects Med. 2011;32:234-46.
Fiorini A, Koudriavtseva T, Bucaj E, Coccia R, Foppoli C, et al. Involvement of oxidative stress in occurrence of relapses in multiple sclerosis: the spectrum of oxidatively modified serum proteins detected by proteomics and redox proteomics analysis. PLoS One. 2013;8:e65184.
Tasset I, Aguera E, Sanchez-Lopez F, Feijoo M, Giraldo AI, et al. Peripheral oxidative stress in relapsing-remitting multiple sclerosis. Clin Biochem. 2012;45:440-4.
Karlik M, Valkovic P, Hancinova V, Krizova L, Tothova L, et al. Markers of oxidative stress in plasma and saliva in patients with multiple sclerosis. Clin Biochem. 2014.
Van der Goes A, Wouters D, Van Der Pol SM, Huizinga R, Ronken E, et al. Reactive oxygen species enhance the migration of monocytes across the blood-brain barrier in vitro. FASEB J. 2001;15: 1852-4.
Wang P, Xie K, Wang C, Bi J. Oxidative Stress Induced by Lipid Peroxidation Is Related with Inflammation of Demyelination and Neurodegeneration in Multiple Sclerosis. Eur Neurol. 2014;72:249-54.
Romme Christensen J, Bornsen L, Khademi M, Olsson T, Jensen PE, et al. CSF inflammation and axonal damage are increased and correlate in progressive multiple sclerosis. Mult Scler. 2013;19:877-84.
Pennisi G, Cornelius C, Cavallaro MM, Salinaro AT, Cambria MT, et al. Redox regulation of cellular stress response in multiple sclerosis. Biochem Pharmacol. 2011;82:1490-9.
Pasquali L, Pecori C, Lucchesi C, LoGerfo A, Iudice A, et al. Plasmatic oxidative stress biomarkers in multiple sclerosis: Relation with clinical and demographic characteristics. Clin Biochem. 2014.
Ortiz GG, Pacheco-Moises FP, Bitzer-Quintero OK, Ramirez-Anguiano AC, Flores-Alvarado LJ, et al. Immunology and oxidative stress in multiple sclerosis: clinical and basic approach. Clin Dev Immunol. 2013:708659.
Lee DH, Gold R, Linker RA. Mechanisms of Oxidative Damage in Multiple Sclerosis and Neurodegenerative Diseases: Therapeutic Modulation via Fumaric Acid Esters. Int J Mol Sci. 2012;13:11783-803.
- Abstract Viewed: 1025 times
- PDF Downloaded: 539 times