The Study of the Protective Effect of Vitamin E and Retinoic Acid on Testicular Tissue in Mice Treated with Cyclophosphamide
Archives of Medical Laboratory Sciences,
Vol. 6 (2020),
20 Dey 2020
,
Page 1-8 (e9)
https://doi.org/10.22037/amls.v6.31520
Abstract
Background and Aim: One of the side effects of anticancer drugs is spermatogenesis disorder. Hence, the evaluation of sperm production after chemotherapy has been the subject of several studies today.
Methods: Adult male albino mice weighing 20±2 g were randomly divided into 5 groups (n = 4);control, cyclophosphamide (12 mg/kg/day), cyclophosphamide and vitamin E (200 mg/kg/day), cyclophosphamide and retinoic acid (500 µL/72h), cyclophosphamide and retinoic acid (250 µL/72h) and vitamin E (100 mg/day). The treatment was continued for 35 days and at the end of the rats was anesthetized and testicular tissue was isolated, weighed, fixed, and stained with hematoxylin-eosin.
Results: A significant decrease (p-value ≤ 0.0001) in the mean diameter of seminal vesicles, epithelial thickness, and index of tubular differentiation was observed in the testis tissue of mice treated with cyclophosphamide compared to the control group. In cyclophosphamide recipients with retinoic acid or vitamin E, parameters significantly increased to the level of the control group (p-value ≤ 0.0001), whereas cyclophosphamide co-administration with retinoic acid and vitamin A had a less tissue-protective effect.
Conclusion: Vitamin E and retinoic acid can offset the adverse effects of cyclophosphamide on spermatogenesis and may be inducers of spermatogenesis, while the combination of these two antioxidants has fewer efficacies.
*Corresponding Author: Fatemeh Sadat Moravej; Email: akbar.karimi@es.isfpnu.ac.ir
Please cite this article as: Karimi A, Behmard V, Toghiani S, Moravej FS. The Study of the Protective Effect of Vitamin E and Retinoic Acid on Testicular Tissue in Mice Treated with Cyclophosphamide. Arch Med Lab Sci. 2020;6:1-8 (e9). https://doi.org/10.22037/amls.v6.31520
- Cyclophosphamide
- Vitamin E
- Retinoic acid
- Spermatogenesis
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References
Meistrich ML, Finch M, da Cunha MF, Hacker U, Au WW. Damaging effects of fourteen chemotherapeutic drugs on mouse testis cells. Cancer research. 1982;42(1):122-31.
Meistrich M, Wilson G, Brown B, Da Cunha M, Lipshultz L. Impact of cyclophosphamide on long‐term reduction in sperm count in men treated with combination chemotherapy for Ewing and soft tissue sarcomas. Cancer. 1992;70(11):2703-12.
Friedman O, OM F. Cyclophosphamide and related phosphoramide mustards. Current status and future prospects. 1979.1:143
Fraiser LH, Kanekal S, Kehrer JP. Cyclophosphamide toxicity. Drugs. 1991;42(5):781-95.
Lombardo F, Sansone A, Romanelli F, Paoli D, Gandini L, Lenzi A. The role of antioxidant therapy in the treatment of male infertility: an overview. Asian Journal of andrology. 2011;13(5):7-690.
Momeni HR, Eskandari N. Effect of vitamin E on sperm parameters and DNA integrity in sodium arsenite-treated rats. Iranian Journal of Reproductive Medicine. 2012;10(3):249.
Zhou DX, Qiu SD, Zhang J, Tian H, Wang HX. The protective effect of vitamin E against oxidative damage caused by formaldehyde in the testes of adult rats. Asian journal of andrology. 2006;8(5):584-8.
Jauregui EJ, Mitchell D, Topping T, Hogarth CA, Griswold MD. Retinoic acid receptor signaling is necessary in steroidogenic cells for normal spermatogenesis and epididymal function. Development. 2018;145(13):160-465.
Fu Z, Kubo T, Sugahara K, Noguchi T, Kato H. Retinoid nutritional status differently affects the expression of Japanese quail retinoic acid receptor-beta isoform transcripts in a tissue-specific manner. Journal of endocrinology. 2001;169(2):281-90.
VAN PELT AM, DE ROOIJ DG. Retinoic acid is able to reinitiate spermatogenesis in vitamin A-deficient rats and high replicate doses support the full development of spermatogenic cells. Endocrinology. 1991;128(2):697-704.
Moore H, Akhondi MA. In vitro maturation of mammalian spermatozoa. Reviews of Reproduction. 1996;1(1):54-60.
Rezvanfar M, Sadrkhanlou R, Ahmadi A, Shojaei-Sadee H, Rezvanfar M, Mohammadirad A, et al. Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Human & experimental toxicology. 2008;27(12):901-10.
Ghojaie M, Barzegar A, Asadzadeh R. The survey of IRFI005 function about removing of intra and extracellular free radicals. scientific journal of ilam university of medical sciences. 2014;22(2):158-66.
Krishnamoorthy G, Venkataraman P, Arunkumar A, Vignesh R, Aruldhas M, Arunakaran J. Ameliorative effect of vitamins (α-tocopherol and ascorbic acid) on PCB (Aroclor 1254) induced oxidative stress in rat epididymal sperm. Reproductive Toxicology. 2007;23(2):239-45.
Tamilselvan P, Bharathiraja K, Vijayaprakash S. Protective role of lycopene on bisphenol A induced changes in sperm characteristics, testicular damage and oxidative stress in rats. 2013.
Shalaby M, El-Zorba H. Protective effect of celery oil, vitamin E and their combination against testicular toxicity in male rats. Global veterinaria. 2010;5(2):122-8.
Ayinde OC, Ogunnowo S, Ogedegbe RA. Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead-exposed albino rats. BMC pharmacology and toxicology. 2012;13(1):1-8.
Livera G, Rouiller-Fabre V, Pairault C, Levacher C, Habert R. Regulation and perturbation of testicular functions by vitamin A. Reproduction-cambridge-. 2002;124(2):173-80.
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