Effect of Quercus infectoria and Zataria multiflora extracts on the expression of Apo-B100 and PPAR-α in liver and adipose tissues in insulin resistant rats
Archives of Medical Laboratory Sciences,
Vol. 4 No. 4 (2018),
16 February 2020
https://doi.org/10.22037/amls.v4i4.24934
Abstract
Background: Insulin resistance can increase the risk of metabolic syndrome. Studies have shown that expression of PPAR alpha improved insulin function in patients with insulin resistance. Also ApoB100 is an essential ligand for the receptors of low-density lipoproteins (LDL). Increased plasma level of apoB100 is a risk factor for cardiovascular disease (CVD) and its increased production leads to insulin resistance. The aim of this study was to evaluate the effects of Q. Infectoria and Z. multiflora extracts on the expression of PPARα and Apo-B100 genes in adipose and hepatic tissues of insulin-resistant rats
Materials and methods: Forty Wistar rats were divided into 1- healthy control, 2- high fat control, 3- fenofibrate,4- Q. Infectoria and 5- Z. multiflora groups. All groups were fed with high fat diet for 6 weeks expect for the healthy control. Glucose tolerance test was performed to confirm insulin resistance in rats. Then groups 3, 4, and 5 were treated by fenofibrate, Q. Infectoria and Z. multiflora extracts respectively. After sacrificing the rats, their liver and fat tissues were removed. Real-time PCR was used to assess PPARα and ApoB100 gene expressions.
Results: All groups had significant weight gain after 8 weeks. Expression of PPAR-α and ApoB100 genes were the same in Q. Infectoria, Z. multiflora, fenofibrate and healthy control groups.
Conclusion: In conclusion, Q. Infectoria and Z. multiflora extracts decreased ApoB100 and increased PPARα gene expressions but these changes were not statistically significant.
- Quercus infectoria
- Zataria multiflora
- insulin resistance
- ApoB100
- PPARα
How to Cite
References
References
Ford ES. The metabolic syndrome and mortality from cardiovascular disease and all-causes: findings from the National Health and Nutrition Examination Survey II Mortality Study, Atherosclerosis. 2004;173(2),307-312.
Aguilar M, Bhuket T, Torres S, Liu B, Wong RJ. Prevalence of the metabolic syndrome in the United States, 2003-2012. Jama. 2015;313(19),1973-1974.
Grundy SM, Cleeman JI, Daniels SR, Donato KA, Eckel RH, Franklin BA, et al. Diagnosis and management of the metabolic syndrome. Circulation. 2005;112(17),2735-2752.
Taghibiglou C, Carpentier A, Van Iderstine SC, Chen B, Rudy D, Aiton A, et al. Mechanisms of Hepatic Very Low Density Lipoprotein Overproduction in Insulin Resistance evidence for enhanced lipoprotein assembly, reduced intracellular apob degradation, and increased microsomal triglyceride transfer protein in a fructose-fed hamster model. Journal of Biological Chemistry. 2000;275(12),8416-8425.
Casaschi A, Maiyoh GK, Adeli K, Theriault AG. Increased diacylglycerol acyltransferase activity is associated with triglyceride accumulation in tissues of diet-induced insulin-resistant hyperlipidemic hamsters. Metabolism. 2005;54(3),403-409.
Borén J, Lee I, Zhu W, Arnold K, Taylor S, Innerarity TL. Identification of the low density lipoprotein receptor-binding site in apolipoprotein B100 and the modulation of its binding activity by the carboxyl terminus in familial defective apo-B100. Journal of Clinical Investigation. 1998;101(5),1084-1093.
Ridker PM, Rifai N, Cook NR, Bradwin G, Buring JE. Non–HDL cholesterol, apolipoproteins AI and B100, standard lipid measures, lipid ratios, and CRP as risk factors for cardiovascular disease in women. Jama. 2005;294(3),326-333.
Patsouris D, Mandard S, Voshol PJ, Escher P, Tan NS, Havekes LM, et al. PPARα governs glycerol metabolism. The Journal of clinical investigation. 2004;114(1),94-103.
Alberti KGMM, Zimmet P, Shaw J. Metabolic syndrome a new world‐wide definition. A consensus statement from the international diabetes federation. Diabetic medicine. 2006;23(5),469-480.
Grundy SM. American Heart Association, National Heart, Lung, and Blood Institute. Definition of metabolic syndrome: report of the National Heart, Lung, and Blood Institute/American Heart Association conference on scientific issues related to definition. Circulation. 2004;109(3),433-438.
Bhandari R, Kelley GA, Hartley TA, Rockett IR. Metabolic syndrome is associated with increased breast cancer risk: a systematic review with meta-analysis. International journal of breast cancer.2014;2014,1-13.
Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, et al. Increased oxidative stress in obesity and its impact on metabolic syndrome. The Journal of clinical investigation. 2004;114(12),1752-1761.
Raghavan S, Subramaniyam G, Shanmugam N. Proinflammatory effects of malondialdehyde in lymphocytes. Journal of leukocyte biology. 2012;92(5),1055-1067.
Meshkani R, Adeli K. Hepatic insulin resistance, metabolic syndrome and cardiovascular disease. Clinical biochemistry. 2009;42(13),1331-1346.
Gholamhoseinian A, Shahouzehi B, Joukar S, Iranpoor M. Effect of Quercus infectoria and Rosa damascena on lipid profile and atherosclerotic plaque formation in rabbit model of hyperlipidemia. Pakistan journal of biological sciences. 2012;15(1),27-33.
Weindl G, Schäfer-Korting M, Schaller M, Korting HC. Peroxisome Proliferator-Activated Receptors and their Ligands. Drugs. 2005;65(14),1919-1934.
Li RW, Douglas TD, Maiyoh GK, Adeli K, Theriault AG. Green tea leaf extract improves lipid and glucose homeostasis in a fructose-fed insulin-resistant hamster model. Journal of ethnopharmacology. 2006;104(1),24-31.
Kianbakht S, Abasi B, Perham M, Hashem Dabaghian F. Antihyperlipidemic Effects of Salvia officinalis L. Leaf Extract in Patients with Hyperlipidemia: A Randomized Double‐Blind Placebo‐Controlled Clinical Trial. Phytotherapy Research. 2011;25(12),1849-1853.
Li RW, Theriault AG, Au K, Douglas TD, Casaschi A, Kurowska EM. Citrus polymethoxylated flavones improve lipid and glucose homeostasis and modulate adipocytokines in fructose-induced insulin resistant hamsters. Life sciences. 2006;79(4),365-373.
Huang TH, Peng G, Kota BP, Li GQ, Yamahara J, Roufogalis BD, et al. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-γ and identification of an active component. Toxicology and applied pharmacology. 2005;207(2),160-169.
Ryu OH, Lee J, Lee KW, Kim HY, Seo JA, Kim SG, et al. Effects of green tea consumption on inflammation, insulin resistance and pulse wave velocity in type 2 diabetes patients. Diabetes research and clinical practice. 2006;71(3),356-358.
- Abstract Viewed: 105 times
- PDF Downloaded: 61 times