Conjugates of B cells and platelet microparticles in the human peripheral blood
Archives of Medical Laboratory Sciences,
Vol. 2 No. 3 (2016),
23 November 2016
https://doi.org/10.22037/amls.v2i3.15160
Abstract
Background: Activated platelets shed microparticles (MPs) in vivo and certainly in vitro under storage. Like platelets, platelet-derived MPs contribute to hemostatic and inflammatory responses. We sought to determine the interactions between platelet MPs and peripheral B lymphocytes in the healthy blood circulation to propose a possible role for platelet MPs in the functioning of B cells.
Materials and Methods: An enzyme-linked immunosorbent assay (ELISA) was established to determine the normal interactions between human peripheral blood B lymphocytes and platelet MPs. B cells were isolated and bound to the wells of microtiter plates using coated anti-CD19. Then the presence of attached MPs was surveyed. Also, platelet MPs were separated from human platelet concentrates and applied to confirm the new binding capacities of B cells for these microvesicles.
Results: Platelet MPs were recognized in the wells of ELISA in which only B cells were isolated. So MPs were bound with peripheral blood B cells. Furthermore, using this method, the role of CD40/ CD40L interaction was displayed for the binding.
Conclusion: It seemed that the binding of platelet MPs to B cells normally took place in vivo and a percent of B cells circulate in blood in connection with platelet MPs.
- ELISA
- Platelet microparticles (Platelet MPs)
- B cells
- CD40L
How to Cite
References
Sprague DL, Elzey BD, Crist SA, Waldschmidt TJ, Jensen RJ, Ratliff TL. Platelet-mediated modulation of adaptive immunity: unique delivery of CD154 signal by platelet-derived membrane vesicles. Blood. 2008;111(10):5028-36.
Cognasse F, Hamzeh-Cognasse H, Lafarge S, Chavarin P, Cogne M, Richard Y, et al. Human platelets can activate peripheral blood B cells and increase production of immunoglobulins. Experimental hematology. 2007;35(9):1376-87.3. Zarbock A, Polanowska-Grabowska RK, Ley K. Platelet-neutrophil-interactions: linking hemostasis and inflammation. Blood reviews. 2007;21(2):99-111.
Elzey BD, Tian J, Jensen RJ, Swanson AK, Lees JR, Lentz SR, et al. Platelet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments. Immunity. 2003;19(1):9-19.
Hagihara M, Higuchi A, Tamura N, Ueda Y, Hirabayashi K, Ikeda Y, et al. Platelets, after exposure to a high shear stress, induce IL-10-producing, mature dendritic cells in vitro. Journal of immunology (Baltimore, Md : 1950). 2004;172(9):5297-303.
Czapiga M, Kirk AD, Lekstrom-Himes J. Platelets deliver costimulatory signals to antigen-presenting cells: a potential bridge between injury and immune activation. Experimental hematology. 2004;32(2):135-9.
Danese S, de la Motte C, Reyes BM, Sans M, Levine AD, Fiocchi C. Cutting edge: T cells trigger CD40-dependent platelet activation and granular RANTES release: a novel pathway for immune response amplification. Journal of immunology (Baltimore, Md : 1950). 2004;172(4):2011-5.
Solanilla A, Pasquet JM, Viallard JF, Contin C, Grosset C, Dechanet-Merville J, et al. Platelet-associated CD154 in immune thrombocytopenic purpura. Blood. 2005;105(1):215-8.
Elzey BD, Grant JF, Sinn HW, Nieswandt B, Waldschmidt TJ, Ratliff TL. Cooperation between platelet-derived CD154 and CD4+ T cells for enhanced germinal center formation. Journal of leukocyte biology. 2005;78(1):80-4.
Nagasawa M, Zhu Y, Isoda T, Tomizawa D, Itoh S, Kajiwara M, et al. Analysis of serum soluble CD40 ligand (sCD40L) in the patients undergoing allogeneic stem cell transplantation: platelet is a major source of serum sCD40L. European journal of haematology. 2005;74(1):54-60.
Khan SY, Kelher MR, Heal JM, Blumberg N, Boshkov LK, Phipps R, et al. Soluble CD40 ligand accumulates in stored blood components, primes neutrophils through CD40, and is a potential cofactor in the development of transfusion-related acute lung injury. Blood. 2006;108(7):2455-62.
Ma DY, Clark EA. The role of CD40 and CD154/CD40L in dendritic cells. Seminars in immunology. 2009;21(5):265-72.
Gatto D, Brink R. The germinal center reaction. The Journal of allergy and clinical immunology. 2010;126(5):898-907; quiz 8-9.
Foy TM, Laman JD, Ledbetter JA, Aruffo A, Claassen E, Noelle RJ. gp39-CD40 interactions are essential for germinal center formation and the development of B cell memory. The Journal of experimental medicine. 1994;180(1):157-63.
Han S, Hathcock K, Zheng B, Kepler TB, Hodes R, Kelsoe G. Cellular interaction in germinal centers. Roles of CD40 ligand and B7-2 in established germinal centers. Journal of immunology (Baltimore, Md : 1950). 1995;155(2):556-67.
Zotos D, Coquet JM, Zhang Y, Light A, D'Costa K, Kallies A, et al. IL-21 regulates germinal center B cell differentiation and proliferation through a B cell-intrinsic mechanism. The Journal of experimental medicine. 2010;207(2):365-78.
Elzey BD, Ratliff TL, Sowa JM, Crist SA. Platelet CD40L at the interface of adaptive immunity. Thrombosis research. 2011;127(3):180-3.
van Kooten C, Banchereau J. CD40-CD40 ligand. Journal of leukocyte biology. 2000;67(1):2-17.
Siljander PR. Platelet-derived microparticles - an updated perspective. Thrombosis research. 2011;127 Suppl 2:S30-3.
Wolf P. The nature and significance of platelet products in human plasma. British journal of haematology. 1967;13(3):269-88.
Mause SF, Weber C. Microparticles: protagonists of a novel communication network for intercellular information exchange. Circulation research. 2010;107(9):1047-57.
Messer L, Alsaleh G, Freyssinet JM, Zobairi F, Leray I, Gottenberg JE, et al. Microparticle-induced release of B-lymphocyte regulators by rheumatoid synoviocytes. Arthritis research & therapy. 2009;11(2):R40.
Janowska-Wieczorek A, Majka M, Kijowski J, Baj-Krzyworzeka M, Reca R, Turner AR, et al. Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment. Blood. 2001;98(10):3143-9.
Boilard E, Nigrovic PA, Larabee K, Watts GF, Coblyn JS, Weinblatt ME, et al. Platelets amplify inflammation in arthritis via collagen-dependent microparticle production. Science (New York, NY). 2010;327(5965):580-3.
Morel O, Toti F, Hugel B, Bakouboula B, Camoin-Jau L, Dignat-George F, et al. Procoagulant microparticles: disrupting the vascular homeostasis equation? Arteriosclerosis, thrombosis, and vascular biology. 2006;26(12):2594-604.
Rubin O, Crettaz D, Tissot JD, Lion N. Microparticles in stored red blood cells: submicron clotting bombs? Blood transfusion = Trasfusione del sangue. 2010;8 Suppl 3:s31-8.
Italiano JE, Jr., Mairuhu AT, Flaumenhaft R. Clinical relevance of microparticles from platelets and megakaryocytes. Current opinion in hematology. 2010;17(6):578-84.
Sinauridze EI, Kireev DA, Popenko NY, Pichugin AV, Panteleev MA, Krymskaya OV, et al. Platelet microparticle membranes have 50- to 100-fold higher specific procoagulant activity than activated platelets. Thrombosis and haemostasis. 2007;97(3):425-34.
Heloire F, Weill B, Weber S, Batteux F. Aggregates of endothelial microparticles and platelets circulate in peripheral blood. Variations during stable coronary disease and acute myocardial infarction. Thrombosis research. 2003;110(4):173-80.
- Abstract Viewed: 647 times
- PDF Downloaded: 568 times