The effect of Migri-Heal® on nitric oxide production in an in vitro inflammatory model of primary microglial cells
Archives of Medical Laboratory Sciences,
Vol. 2 No. 2 (2016),
16 Shahrivar 2016
https://doi.org/10.22037/amls.v2i2.14529
Abstract
Background: Recently, much attention has been directed towards considering activated microgelial cells as putative targets for treatment of neurological disorders. MigriHeal® as a novel herbal remedy was introduced for the treatment of migraine headaches. The previous researches has shown that MigriHeal® extracts can decrease NO in an in vitro inflammatory model. The aim of this study was to investigate the effect of MigriHeal® on NO generation from LPS- stimulated microglia cells.
Materials and Methods: Neonatal rat primary microglial cells were isolated from the mixed glial cultures and the purity of the cultures was determined by immunocytochemistry. Microglial cells were pretreated with Migri-Heal® and activated by 1μg/ml LPS. Subsequently, NO levels in the culture supernatants were measured by a griess reaction. Our results showed that Migri-Heal® 50μg/ml significantly reduced NO level in inflamed microglia in a dose-dependent manner. Results: The results showed that different concentrations of Migri-Heal® had no prominent effect on cell viability in presence of LPS as compared with the control group. In addition, the pretreatment of microglia cells with Migri-Heal® can prevent from a morphological changes of the cells into the round and phagocytic shape. Conclusion: Our study demonstrated that MigriHeal® might have NO scavenging properties. Integrative studies are warranted to uncover the novel pharmacological insights of this herbal remedy as an putative therapeutic approach against diseases - associated with inflammation.
- inflammation
- microglia
- Migri-Heal®
- nitric oxide
How to Cite
References
Allen N Ja, Barres BA. Signaling between glia and neurons: focus on synaptic plasticity. Current Opinion in Neurobiology. (2005);15:542–8.
Geppert TD, Whitehurst CE, Thompson P, Beutler B. Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras/raf-1/MEK/MAPK pathway. Mol Med. 1994;1(1):93-103.
Napoli I, Neumann H. Microglial clearance function in health and disease. Neuroscience. 2009;158(3):1030-8.
Hanisch UK. Microglia as a source and target of cytokines. Glia. 2002;40(2):140-55.
Wilms H, Zecca L, Rosenstiel P, Sievers J, Deuschl G, Lucius R. Inflammation in Parkinson's diseases and other neurodegenerative diseases: cause and therapeutic implications. Current pharmaceutical design. 2007;13(18):1925-8.
McCarthy KD, de Vellis J. Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. The Journal of cell biology. 1980;85(3):890-902.
Naimi SM. Study of the effects of seven medicinal herbs waters upon nitric oxide production in cultured vascular endotheliuma cells. Islamic Azad University. Tehran, Iran.: Islamic Azad University. Tehran,; 2007.
Ansari A. Effects of three Medicinal Herbs Essontial oil on Nittric Oxide Production in Cultured vascular endothelioma cell line: Tehran University of Medical Sciences.; 2008.
Rafieea kh AM, Mahdianc R, Paknejada M, Fallah MS, Azizi M. in vitro Effects of a Herbal Remedy for Migraine Treatment, MigriHeal®, on
Basal and LPS-induced Nitric Oxide. J Basic Appl Sci Res,. 2013;3(5):206-11.
McCarthy KDaDV, J. . Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. Journal of Cell Biology. (1980) 85: 890-902.
CHANG ea. Influence of neurons on lipopolysaccharide-stimulated production of nitric oxide and tumor necrosis factor-alpha by cultured glia. Brain Research. 2000;853:236-44.
Hanisch UK, Kettenmann H. Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nature neuroscience. 2007;10(11):1387-94.
Neumann H, Kotter MR, Franklin RJ. Debris clearance by microglia: an essential link between degeneration and regeneration. Brain : a journal of neurology. 2009;132(Pt 2):288-95.
Amor S, Puentes F, Baker D, van der Valk P. Inflammation in neurodegenerative diseases. Immunology. 2010;129(2):154-69.
Nakamura Y, Si QS, Kataoka K. Lipopolysaccharide-induced microglial activation in culture: temporal profiles of morphological change and release of cytokines and nitric oxide. Neuroscience research. 1999;35(2):95-100.
Pahan K, Sheikh FG, Liu X, Hilger S, McKinney M, Petro TM. Induction of nitric-oxide synthase and activation of NF-kappaB by interleukin-12 p40 in microglial cells. The Journal of biological chemistry. 2001;276(11):7899-905.
Olesen J. The role of nitric oxide (NO) in migraine, tension-type headache and cluster headache. Pharmacology & therapeutics. 2008;120(2):157-71.
Brun J, Claustrat B, Saddier P, Chazot G. Nocturnal melatonin excretion is decreased in patients with migraine without aura attacks associated with menses. Cephalalgia. 1995;15(2):136-9; discussion 79.
Sarchielli P, Alberti A, Codini M, Floridi A, Gallai V. Nitric oxide metabolites, prostaglandins and trigeminal vasoactive peptides in internal jugular vein blood during spontaneous migraine attacks. Cephalalgia. 2000;20(10):907-18.
Bastos VP, Gomes AS, Lima FJ, Brito TS, Soares PM, Pinho JP, et al. Inhaled 1,8-cineole reduces inflammatory parameters in airways of ovalbumin-challenged Guinea pigs. Basic & clinical pharmacology & toxicology. 2011;108(1):34-9.
Juergens UR, Stober M, Vetter H. Inhibition of cytokine production and arachidonic acid metabolism by eucalyptol (1.8-cineole) in human blood monocytes in vitro. European journal of medical research. 1998;3(11):508-10.
Juergens UR, Stober M, Schmidt-Schilling L, Kleuver T, Vetter H. Antiinflammatory effects of euclyptol (1.8-cineole) in bronchial asthma: inhibition of arachidonic acid metabolism in human blood monocytes ex vivo. European journal of medical research. 1998;3(9):407-12.
Juergens UR, Engelen T, Racke K, Stober M, Gillissen A, Vetter H. Inhibitory activity of 1,8-cineol (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes. Pulmonary pharmacology & therapeutics. 2004;17(5):281-7.
Liang D, Li F, Fu Y, Cao Y, Song X, Wang T, et al. Thymol inhibits LPS-stimulated inflammatory response via down-regulation of NF-kappaB and MAPK signaling pathways in mouse mammary epithelial cells. Inflammation. 2014;37(1):214-22.
Braga PC, Dal Sasso M, Culici M, Bianchi T, Bordoni L, Marabini L. Anti-inflammatory activity of thymol: inhibitory effect on the release of human neutrophil elastase. Pharmacology. 2006;77(3):130-6.
Aydogan S, Yerer MB, Goktas A. Melatonin and nitric oxide. Journal of endocrinological investigation. 2006;29(3):281-7.
Ansari M, Rafiee Kh, Yasa N, Vardasbi S, Naimi SM, Nowrouzi, A. Measurement of melatonin in alcoholic and hot water extracts of Tanacetum parthenium, Tripleurospermum disciforme and Viola odorata. Daru,. 2010;18,((3):):p. 173-8.
Nakajima K, Tohyama Y, Kohsaka S, Kurihara T. Protein kinase C alpha requirement in the activation of p38 mitogen-activatedprotein kinase, which is linked to the induction of tumor necrosis factor alpha in lipopolysaccharide-stimulated microglia. Neurochemistry international. 2004;44(4):205-14.
Suzumura A, Marunouchi T, Yamamoto H. Morphological transformation of microglia in vitro. Brain Res. 1991;545(1-2):301-6.
- Abstract Viewed: 1473 times
- PDF Downloaded: 384 times