In vitro cytotoxic effect of urtica dioica extracts on acute myelogenous leukemia cell line (kg-1)
Archives of Medical Laboratory Sciences,
Vol. 2 No. 1 (2016),
9 Farvardin 2016
https://doi.org/10.22037/amls.v2i1.13689
Abstract
Background: Urtica dioica is one of the medicinal herbs with many uses in treating various diseases. In some studies, its antiproliferative and apoptotic effects on cancer cell lines have been shown. Therefore, the evaluation of U. dioica effect was performed on KG-1 cell line for acute myelogenous leukemia (AML) for the first time in this study.
Materials and Methods: KG-1 cell line was treated by various extracts (aqueous, hydroalcoholic, chloroform and ethyl acetate) of U. dioica aerial parts and roots in different concentrations. Metabolic activity of extracts on cell line was assessed by MTT assay. To evaluate the percentage of apoptotic cells, the flow cytometry was performed by FITC Annexin V-PI apoptosis detection kit in KG-1 cell line treated with root chloroform (UDC-R) and ethyl acetate (UDE-R) extracts. The results have been reported as percentage of cell viability and IC50.
Results: Based on MTT results, the strongest IC50 in KG-1 cell line (219.361μg/ml) was related to UDC-R. The flow cytometric analysis showed that UDC-R and UDE-R in IC50 concentration induced early (53.6% and 57.4%, respectively) and late (27% and 33.2%, respectively) apoptosis in KG-1 cells after 24 hrs. The inhibition of cell proliferation by various extracts of U. dioica was dependent on concentration (p=0.000).
Conclusion: Flow cytometric analysis confirmed that UDC-R and UDE-R extracts affect on proliferation reduction of KG-1 cells by activating the apoptotic pathway.
- Urtica dioica extract
- acute myelogenous leukemia
- KG-1
- IC50
- apoptosis
How to Cite
References
Hoffbrand V, Moss PAH. Hoffbrand’s essential haematology. Seventh Edition: Wiley-Blackwell. 2016:123-124.
Huang XJ, Ren W, Li J, Chen LY, Mei ZN. Anti-inflammatory and anticancer activities of ethanol extract of Pendulous monkshood root in vitro. Asian Pacific J Cancer Prev. 2013;14(6):3569-3573.
Fattahi S, Motevalizadeh Ardekani A, Zabihi E, Abedian Z, Mostafazadeh A, Pourbagher R, et al. Antioxidant and apoptotic effects of an aqueous extract of Urtica dioica on the MCF-7 human breast cancer cell line. Asian Pacific Journal of Cancer Prevention. 2013;14(9):5317-23.
Olaku O, White JD. Herbal therapy use by cancer patients: A literature review on case reports. Eur J Cancer. 2011;47(4):508-14.
WHO monographs on selected medicinal plants. World Health Organization, Geneva; 2002;2:332-334.
Haj Said A, Sabi El Otmani E, Derfoufi S, Benmoussa A. Highlights on nutritional and therapeutic value of stinging nettle (Urtica dioica). International Journal of Pharmacy and Pharmaceutical Sciences. 2015;7(10):8-14.
Ahmad Dar S, Ahmad Ganai F, Yousuf AE, Balkhi MH, Mohsin Bhat T, Sharma P. Pharmacological and toxicological evaluation of Urtica dioica. Pharmaceutical Biology. 2013;51(2):170–180.
Apak R, Güçlü K, Demirata B, Özyürek M, Çelik SE, Bektaşoğlu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007;12:1496-547.
Fattahi S, Zabihi E, Zeinab Abedian, et al. Total phenolic and flavonoid contents of aqueous extract of stinging nettle and in vitro antiproliferative effect on Hela and BT-474 cell lines. IJMCM. 2014;3(2):102-107.
Stockerta JC, Blázquez-Castroa A, Cañetea M, Horobinb RW, Villanuevaa A. MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. Acta Histochemica. 2012;114(8):785–96.
Lingadurai S, Roy S, Joseph RV, Nath LK. Antileukemic activity of the leaf extract of Bischofia javanica blume on human leukemic cell lines. Indian J Pharmacol. 2011;43(2):143–9.
Golstein P, Kroemer G. Cell death by necrosis: towards amolecular definition. TRENDS in Biochemical Sciences. 2006;32(1).
Elmore S. Apoptosis: A Review of programmed cell death. Toxicologic Patholog. 2007;35:495–516.
Collins JA, Schandl CA, Young KK, Vesely J, Willingham MC. Major DNA fragmentation is a late event in apoptosis. The Journal of Histochemistry & Cytochemistry. 1997;45(7):923–34.
Demchenko AP. Beyond annexin V: fluorescence response of cellular membranes to apoptosis. Cytotechnology 2012;65:157–72.
Mariño G, Kroemer G. Mechanisms of apoptotic phosphatidylserine exposure. Cell Research. 2013;23:1247-8.
Rieger AM, Nelson KL, Konowalchuk JD, Barreda DR. Modified Annexin V/Propidium Iodide apoptosis assay For accurate assessment of cell death. J. Vis. Exp. 2011(50).
Logue SE, Elgendy M, Martin SJ. Expression, purification and use of recombinant annexin V for the detection of apoptotic cells. Nature Protocols. 2009;4:1383-95.
Lawen A. Apoptosis—an introduction. BioEssays Wiley Periodicals, Inc. 2003;25:888–96.
Güder A, Korkmaz H. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iranian Journal of Pharmaceutical Research. 2012;11(3):913-23.
Konrad L, Muller HH, Lenz C, Laubinger H, Aumuller G, Lichius JJ. Antiproliferative effect on human prostate cancer cells by a stinging nettle root (Urtica dioica) extract. Placenta medica. 2000;66:44-7.
Martins dos Santos H, Oliveira DF, Antonio de Carvalho D, Andrade Pinto JM, Costa Campos VA, Braga Moura˜o AR, et al. Evaluation of native and exotic Brazilian plants for anticancer activity. J Nat Med. 2010;64:231–8.
Nahata A, Saxena A, Suri N, Saxena AK, Dixit VK. Sphaeranthus indicus induces apoptosis through mitochondrial-dependent pathway in HL-60 cells and exerts cytotoxic potential on several human cancer cell lines. Integrative Cancer Therapies. 2012;12(3):236–47.
- Abstract Viewed: 715 times
- PDF Downloaded: 497 times