Frequency of Beijing family of Mycobacterium tuberculosis in Mashhad, Northeast of Iran
Archives of Medical Laboratory Sciences,
Vol. 2 No. 3 (2016),
23 Azar 2016
https://doi.org/10.22037/amls.v2i3.15661
Abstract
Background: Beijing family of Mycobacterium tuberculosis (M. tuberculosis) is widespread in Asia and has been involved in outbreaks of multidrug-resistant tuberculosis in various parts of the world. The aim of the current study was to evaluate the frequency of Beijing family of M. tuberculosis in patients with tuberculosis in Mashhad, Iran.
Materials and Methods: Totally, 72 specimens of M. tuberculosis were collected from pulmonary samples of patients at Ghaem hospital in Mashhad (Iran) between April 2011 and May 2012. The authors used IS6110-based polymerase chain reaction (IS6110-based PCR) method to identify Beijing family of M. tuberculosis. Based on PCR results, strains belonging to Beijing and non-Beijing families were detected. Also, among members of Beijing family, ancient and modern subfamilies were distinguished.
Results: Beijing genotype was observed in five (6.9%) of 72 culture positive samples. In the present study, no cases of modern subfamilies were detected. M. tuberculosis had a higher frequency in men (61.1%) compared to that of women (38.9%). Our data demonstrated that IS6110-based PCR can be used to distinguish Beijing family from non-Beijing family, with high specificity and sensitivity.
Conclusion: The improvement of convenient and quick methods to detect and control Beijing family of M. tuberculosis in clinical samples is an interesting subject in areas where M. tuberculosis is prevalent. This method has the advantages of being quick, cost-effective, and requires comparatively less clinical laboratory equipment.
- Mycobacterium tuberculosis
- Beijing lineage
- Polymerase chain reaction
How to Cite
References
Li GL, Zhao DF, Xie T, Ju HF, Mu C, Zhao H, et al. Molecular characterization of drug-resistant Beijing family isolates of Mycobacterium tuberculosis from Tianjin, China. Biomed Environ Sci. 2010;23(3):188-93.
Milian-Suazo F, Perez-Guerrero L, Arriaga-Diaz C, Escartin-Chavez M. Molecular epidemiology of human cases of tuberculosis by Mycobacterium bovis in Mexico. Prev Vet Med. 2010;97(1):37-44.
Novais RC, Borsuk S, Dellagostin OA, Thorstenson YR. Molecular inversion probes for sensitive detection of Mycobacterium tuberculosis. J Microbiol Methods. 2008;72(1):60-6.
Fenton MJ, Vermeulen MW, Kim S, Burdick M, Strieter RM, Kornfeld H. Induction of gamma interferon production in human alveolar macrophages by Mycobacterium tuberculosis. Infect Immun. 1997;65(12):5149-56.
Jiang X, Lu C, Gao F, Wang F, Zhang W, Portugal I, et al. A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP. Microbes and Infection. 2009;11(3):419-23.
Borgdorff MW, Nagelkerke NJ, de Haas PE, van Soolingen D. Transmission of Mycobacterium tuberculosis depending on the age and sex of source cases. Am J Epidemiol. 2001;154(10):934-43.
Niemann S, Rüsch-Gerdes S, Richter E, Thielen H, Heykes-Uden H, Diel R. Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosis Strains in Actual Chains of Transmission. J Clin Microbiol. 2000;38(7):2563-7.
Coscolla M, Gagneux S. Does M. tuberculosis genomic diversity explain disease diversity? Drug Discov Today Dis Mech. 2010;7(1):e43-e59.
Bifani PJ, Mathema B, Kurepina NE, Kreiswirth BN. Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol. 2002;10(1):45-52.
Lopez B, Aguilar D, Orozco H, Burger M, Espitia C, Ritacco V, et al. A marked difference in pathogenesis and immune response induced by different Mycobacterium tuberculosis genotypes. Clin Exp Immunol. 2003;133(1):30-7.
Bifani PJ, Plikaytis BB, Kapur V, Stockbauer K, Pan X, Lutfey ML, et al. Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. Jama. 1996;275(6):452-7.
Agerton TB, Valway SE, Blinkhorn RJ, Shilkret KL, Reves R, Schluter WW, et al. Spread of strain W, a highly drug-resistant strain of Mycobacterium tuberculosis, across the United States. Clin Infect Dis. 1999;29(1):85-92.
Glynn JR, Whiteley J, Bifani PJ, Kremer K, van Soolingen D. Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis: a systematic review. Emerg Infect Dis. 2002;8(8):843-9.
Dou HY, Tseng FC, Lu JJ, Jou R, Tsai SF, Chang JR, et al. Associations of Mycobacterium tuberculosis genotypes with different ethnic and migratory populations in Taiwan. Infect Genet Evol. 2008;8(3):323-30.
Wada T, Fujihara S, Shimouchi A, Harada M, Ogura H, Matsumoto S, et al. High transmissibility of the modern Beijing Mycobacterium tuberculosis in homeless patients of Japan. Tuberculosis. 2009;89(4):252-5.
Mokrousov I, Ly HM, Otten T, Lan NN, Vyshnevskyi B, Hoffner S, et al. Origin and primary dispersal of the Mycobacterium tuberculosis Beijing genotype: clues from human phylogeography. Genome Res. 2005;15(10):1357-64.
Mokrousov I, Narvskaya O, Otten T, Vyazovaya A, Limeschenko E, Steklova L, et al. Phylogenetic reconstruction within Mycobacterium tuberculosis Beijing genotype in northwestern Russia. Res Microbiol. 2002;153(10):629-37.
Borgdorff MW, Nagelkerke NJ, Dye C, Nunn P. Gender and tuberculosis: a comparison of prevalence surveys with notification data to explore sex differences in case detection. Int J Tuberc Lung Dis. 2000;4(2):123-32.
van Soolingen D, Qian L, de Haas PE, Douglas JT, Traore H, Portaels F, et al. Predominance of a single genotype of Mycobacterium tuberculosis in countries of east Asia. J Clin Microbiol. 1995;33(12):3234-8.
Kurepina N, Likhoshvay E, Shashkina E, Mathema B, Kremer K, van Soolingen D, et al. Targeted hybridization of IS6110 fingerprints identifies the W-Beijing Mycobacterium tuberculosis strains among clinical isolates. J Clin Microbiol. 2005;43(5):2148-54.
Anargyros P, Astill DS, Lim IS. Comparison of improved BACTEC and Lowenstein-Jensen media for culture of mycobacteria from clinical specimens. J Clin Microbiol. 1990;28(6):1288-91.
Rasolofo Razanamparany V, Quirin R, Rapaoliarijaona A, Rakotoaritahina H, Vololonirina EJ, Rasolonavalona T, et al. Usefulness of restriction fragment length polymorphism and spoligotyping for epidemiological studies of Mycobacterium bovis in Madagascar: description of new genotypes. Vet Microbiol. 2006;114(1-2):115-22.
Afghani B, Stutman HR. Polymerase chain reaction for diagnosis of M. tuberculosis: comparison of simple boiling and a conventional method for DNA extraction. Biochem Mol Med. 1996;57(1):14-8.
Eing BR, Becker A, Sohns A, Ringelmann R. Comparison of Roche Cobas Amplicor Mycobacterium tuberculosis Assay with In-House PCR and Culture for Detection of M. tuberculosis. J Clin Microbiol. 1998;36(7):2023-9.
Kremer K, Glynn JR, Lillebaek T, Niemann S, Kurepina NE, Kreiswirth BN, et al. Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers. J Clin Microbiol. 2004;42(9):4040-9.
Rohani M, Farnia P, Nasab MN, Moniri R, Torfeh M, Amiri M. Beijing genotype and other predominant Mycobacterium tuberculosis spoligotypes observed in Mashhad city, Iran. Indian journal of medical microbiology. 2009;27(4):306.
Filliol I, Driscoll J, van Soolingen D, al e. Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study. J Clin Microbiol. 2003;41(5):7.
Lillebaek T, Andersen ÅB, Dirksen A, Glynn JR, Kremer K. Mycobacterium tuberculosis Beijing Genotype1. Emerg InfecT Dis. 2003;9(12):1553.
Ojo OO, Sheehan S, Corcoran DG, Nikolayevsky V, Brown T, O'Sullivan M, et al. Molecular epidemiology of Mycobacteriumtuberculosis clinical isolates in Southwest Ireland. Infect Genet Evol. 2010;10(7):1110-6.
Tian L, Si H, Mu T, Fan W, Wang J, Jiang W, et al. Molecular epidemiology of Mycobacterium tuberculosis in Gansu province of China. Chin Med J. 2012;125(19):3458-64.
Wada T, Iwamoto T. Allelic diversity of variable number of tandem repeats provides phylogenetic clues regarding the Mycobacterium tuberculosis Beijing family. Infect Genet Evol. 2009;9(5):921-6.
Rohani M, Farnia P, Nasab MN, Moniri R, Torfeh M, Amiri M. Beijing genotype and other predominant Mycobacterium tuberculosis spoligotypes observed in Mashhad city, Iran. India J Med Microbiol. 2009;27(4):306.
Tsolaki AG, Gagneux S, Pym AS, Goguet de la Salmoniere YO, Kreiswirth BN, Van Soolingen D, et al. Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis. J Clin Microbiol. 2005;43(7):3185-91.
Warren RM, Victor TC, Streicher EM, Richardson M, Beyers N, Gey van Pittius NC, et al. Patients with active tuberculosis often have different strains in the same sputum specimen. Am J Respir Crit Care Med. 2004;169(5):610-4.
Mokrousov I, Jiao WW, Valcheva V, Vyazovaya A, Otten T, Ly HM, et al. Rapid detection of the Mycobacterium tuberculosis Beijing genotype and its ancient and modern sublineages by IS6110-based inverse PCR. J Clin Microbiol. 2006;44(8):2851-6.
Glennon M, Jager B, Dowdall D, Maher M, Dawson M, Quigley F, et al. PCR-based fingerprinting of Mycobacterium bovis isolates. Vet Microbiol. 1997;54(3-4):235-45.
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