Efficacy of Ethanol and Intravenous Lipid Emulsion on Methanol Toxicity in Rats: Biochemical and Histopathological Insights
International Journal of Medical Toxicology and Forensic Medicine,
Vol. 15 No. 03 (2025),
22 September 2025
,
Page 1-10
https://doi.org/10.22037/ijmtfm.v15i03.49410
Abstract
Background: This study evaluated the therapeutic efficacy and interactions of intravenous lipid emulsion (ILE) and ethanol in methanol toxicity, focusing on biochemical parameters, organ function, and histopathology.
Methods: Sixty-four male rats were assigned to seven groups: control, methanol, methanol + ethanol, methanol + ILE, methanol + ethanol + ILE, ethanol, and ILE. Methanol (4 g/kg) was administered orally, followed by ethanol (1 g/kg) an hour later. ILE (1.5 ml/kg) was given within 30 minutes of methanol ingestion, with two additional doses every six hours. After five days, blood and organ samples were analyzed.
Results: Ethanol significantly reduced ALT, AST, and ALP, while LDH was lowest in the methanol + ethanol + ILE group. Ethanol improved kid`ney function (p<0.001), whereas ILE alone increased creatinine. No significant differences were observed in 8-OHdG (p=0.572). Histopathology showed ethanol reduced liver congestion (p=0.0067), lung inflammation (p=0.0186), and congestion (p=0.0067). The combined therapy showed no significant advantage over ethanol alone.
Conclusion: Ethanol remains the most effective treatment, while ILE’s role appears complex and timing-dependent, warranting further investigation.
- Methanol intoxication
- Ethanol
- Intravenous lipid emulsion
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References
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