Shahid Beheshti University of Medical Sciences, Iran
  • Register
  • Login

International Journal of Medical Toxicology and Forensic Medicine

  • About
    • About the Journal
    • Focus and Scope
    • Editorial Team
    • Contact
  • Issues
    • Current
    • Archives
  • Indexing & Abstracting
  • Editorial Policies
    • Peer Review Process
    • Ethical Codes: Medical Research
    • Principles of Publishing Ethics
    • Conflicts of Interest
    • Publication Frequency
    • Open Access Policy
    • CrossMark Policy
    • Article Processing Charges
    • Privacy Statement
  • Announcements
  • Register
  • Login
Advanced Search
  1. Home
  2. Archives
  3. Vol. 12 No. 3 (2022): International Journal of Medical Toxicology and Forensic Medicine (In press)
  4. Original Article (Forensic Medicine)

ISSN: 2251-8762

International Journal of Medical Toxicology and Forensic Medicine (In press)
Vol. 12 No. 3 (2022)

α-Globin Messenger Ribonucleic Acid as a Molecular Marker for Determining the Age of Human Blood Spots in Different Temperatures

  • Heba Abdullah Mostafa Eid
  • Usama Mohammed Ibrahim Elbarrany
  • Sherien S. Ghaleb
  • Mohamed Adly Mohamed
  • Dina Sabry Abdelfattah
  • Heba Mohamed Aboubakr

International Journal of Medical Toxicology and Forensic Medicine, Vol. 12 No. 3 (2022), , Page 37096
https://doi.org/10.32598/ijmtfm.vi.37096 Published 25 May 2022

  • View Article
  • Download
  • Cite
  • References
  • Statastics
  • Share

Abstract

Background: Analyzing recovered evidence, such as blood which is one of the most encountered types of biological evidence, can provide information to establish the definite time when a crime was committed. This study aims to investigate the time- and temperature-related effects on human bloodstain’s α-globin messenger RNA expression and to estimate the bloodstain’s age using α-globin mRNA.
Methods: A total of 22 blood samples were collected from healthy middle-aged volunteers (12 women and 10 men). After preparation, the samples were exposed to temperatures of 4°C, 24°C, and 40°C. Next, the mRNA expression of the α-globin gene was quantified by real-time RT-PCR at different time intervals of 0, 30, 90, and 150 days.
Results: The α-globin gene expression showed the highest mean values by 0 day and at 4°C and the lowest mean values by 150 days and at 40°C. Samples from male participants showed higher mean values of α-globin gene expression compared to their female counterparts. A significant negative correlation was detected between α-globin gene expression and time interval. Meanwhile, a regression equation was formulated to estimate the time interval using the α-globin gene concentration.
Conclusion: α-Globin mRNA could be a useful marker to estimate the age of human blood spots.

Keywords:
  • Blood spot age
  • mRNA α-globin gene
  • Reverse Transcriptase PCR
  • Different time intervals
  • Different temperatures
  • pdf

How to Cite

Eid, H. A. M., Elbarrany, U. M. I., Ghaleb, S. S., Mohamed, M. A., Abdelfattah, D. S., & Aboubakr, H. M. (2022). α-Globin Messenger Ribonucleic Acid as a Molecular Marker for Determining the Age of Human Blood Spots in Different Temperatures. International Journal of Medical Toxicology and Forensic Medicine, 12(3), 37096. https://doi.org/10.32598/ijmtfm.vi.37096
  • ACM
  • ACS
  • APA
  • ABNT
  • Chicago
  • Harvard
  • IEEE
  • MLA
  • Turabian
  • Vancouver
  • Endnote/Zotero/Mendeley (RIS)
  • BibTeX

References

Edelman G, Aalders MCG. Blood degradation and bloodstain age estimation. In: Schotsmans EMJ, Márquez-Grant N, Forbes SL. Taphonomy of human remains: forensic analysis of the dead and the depositional environment: Forensic analysis of the dead and the depositional environment. Hoboken: John Wiley & Sons Ltd; 2017. [DOI:10.1002/9781118953358]

Lin H, Zhang Y, Wang Q, Li B, Huang P, Wang Z. Estimation of the age of human bloodstains under the simulated indoor and outdoor crime scene conditions by ATR-FTIR spectroscopy. Scientific Reports. 2017; 7(1):13254. [DOI:10.1038/s41598-017-13725-1] [PMID] [PMCID]

Ping J, He Z, Liu J, Xie X. Smartphone-based colorimetric chiral recognition of ibuprofen using aptamers-capped gold nanoparticles. Electrophoresis. 2018; 39(3):486-95. [DOI:10.1002/elps.201700372] [PMID]

Weber AR, Lednev IK. Crime clock-analytical studies for approximating time since deposition of bloodstains. Forensic Chemistry. 2020; 19:100248. [DOI:10.1016/j.forc.2020.100248]

Anderson S, Howard B, Hobbs GR, Bishop CP. A method for determining the age of a bloodstain. Forensic Science International. 2005; 148(1):37-45. [DOI:10.1016/j.forsciint.2004.04.071] [PMID]

Inoue H, Takabe F, Iwasa M, Seko Y. Identification of fetal hemoglobin and simultaneous estimation of bloodstain age by high-performance liquid chromatography. International Journal of Legal Medicine. 1991; 104(3):127-31. [DOI:10.1007/BF01369715] [PMID]

Chan YH. Biostatistics 102: Quantitative data--parametric & non-parametric tests. Singapore Medical Journal. 2003; 44(8):391-6. [PMID]

Chan YH. Biostatistics 104: Correlational analysis. Singapore Medical Journal. 2003; 44(12):614-9. [PMID]

Chan YH. Biostatistics 201: Linear regression analysis. Singapore Medical Journal. 2004; 45(2):55-61. [PMID]

Tuckprakhon U, Lomthaisong K, Daduang S, Lomthaisong K. Preliminary study of alpha-globin mRNA as a molecular tool for age estimation of human bloodstains. Walailak Journal of Science and Technology. 2016; 13(4): 281-90. [Link]

Alrowaithi MA, McCallum NA, Watson ND. A method for determining the age of a bloodstain. Forensic Science International. 2014; 234:e30-1. [DOI:10.1016/j.forsciint.2013.11.008] [PMID]

Bauer M. RNA in forensic science. Forensic Science International. Genetics. 2007; 1(1):69-74. [DOI:10.1016/j.fsigen.2006.11.002] [PMID]

Alaeddini R, Walsh SJ, Abbas A. Molecular studies of time-and environment dependent effects on bone DNA survival. Australian Journal of Forensic Sciences. 2010; 42(3):211-20. [DOI:10.1080/00450611003758264]

Rebecchi L, Cesari M, Altiero T, Frigieri A, Guidetti R. Survival and DNA degradation in anhydrobiotic tardigrades. Journal of Experimental Biology. 2009; 212(24):4033-9. [DOI:10.1242/jeb.033266] [PMID]

Zhu Y, Dong YC, Liang WB, Zhang L. [Relationship between RNA degradation and postmortem interval in mice (Chinese)]. Fa Yi Xue za Zhi. 2011; 27(3):161-3. https://pubmed.ncbi.nlm.nih.gov/21899002/[PMID]

Harrison PJ, Heath PR, Eastwood SL, Burnet PW, McDonald B, Pearson RC. The relative importance of premortem acidosis and postmortem interval for human brain gene expression studies: Selective mRNA vulnerability and comparison with their encoded proteins. Neuroscience Letters. 1995; 200(3):151-4. [DOI:10.1016/0304-3940(95)12102-A]

Ross BM, Knowler JT, McCulloch J. On the stability of messenger RNA and ribosomal RNA in the brains of control human subjects and patients with Alzheimer’s disease. Journal of Neurochemistry. 1992; 58(5):1810-19. [DOI:10.1111/j.1471-4159.1992.tb10057.x] [PMID]

Deng W, Lv M, Wang L, Bai P, Liang W, Zhang L. mRNA degradation pattern analysis in post-mortem normalized using the DNA. Forensic Science International. 2013; 4(1):e266-7. [DOI:10.1016/j.fsigss.2013.10.136]

Qi B, Kong L, Lu Y. Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days. Journal of Forensic and Legal Medicine. 2013; 20(4):321-5. [DOI:10.1016/j.jflm.2012.09.014] [PMID]

Preece P, Cairns NJ. Quantifying mRNA in postmortem human brain: Influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance. Molecular Brain Research. 2003; 118(1-2): 60-71. [DOI:10.1016/S0169-328X(03)00337-1]

Sakurada K, Akutsu T, Watanabe K, Miyasaka S, Kasai K. [Identification of body fluid stains using real-time RTPCR. Discrimination between salivary, nasal blood and vaginal secretions (Japanese)]. Japanese Journal of Forensic Science and Technology. 2013; 18(1):1-11. [DOI: 10.3408/jafst.18.1]

Juusola J, Ballantyne J. mRNA profiling for body fluid identification by multiplex quantitative RT-PCR”. Journal of Forensic Sciences. 2007; 52(6):1252-62. [DOI:10.1111/j.1556-4029.2007.00550.x] [PMID]

  • Abstract Viewed: 35 times
  • pdf Downloaded: 35 times

Download Statastics

  • Linkedin
  • Twitter
  • Facebook
  • Google Plus
  • Telegram
Open Journal Systems
Information
  • For Readers
  • For Authors
  • For Librarians
Browse
  • Home
  • Archives
  • Submissions
  • About the Journal
  • Editorial Team
  • Contact

   International Journal of Medical Toxicology and Forensic Medicine (IJMTFM)

                                                  Print ISSN: 2251-8762

                                                     Online ISSN: 2251-8770

The template of this website is designed by Sinaweb