Construction Of Cerium Oxide Nanoparticles And Their Cytotoxicity Evaluation In Vitro And In Vivo
International Journal of Medical Toxicology and Forensic Medicine,
20 September 2021
Nanotechnology plays a significant role in medicine, especially in diagnosis and treatment as a carrier to drugs and vaccinology. Recently, several studies were conducted on the use of nanoparticles as an adjuvant. The main aim of this study was in vivo and in vitro toxicity evaluation of synthesized cerium nanoparticles.
Materials and methods
In the present study, cerium nanoparticles were prepared by using the wet chemical method. The formation of cerium nanoparticles was confirmed by scanning electron microscopy, transmission electron microscopes, x-ray diffraction analysis, dynamic light scattering. In vivo and in vitro toxicity of synthesized nanoparticles was evaluated in three different amounts of cerium nanoparticles (30 µg, 50 µg, and 100 µg) in mice and human fibroblast cell lines, respectively.
Cerium nanoparticles were successfully synthesized, and the identity was confirmed by x-ray diffraction analysis. The shape and size of nanoparticles were spherical and less than 100 nm, respectively. The prepared nanoparticles had a charge of -26.6 mV and a hydrodynamic radius of 446 nm. MTT assay showed that none of the concentration of cerium was toxic, and in vivo toxicity also clarified the safety of cerium nanoparticles in mice and no significant un-normal behavioural and physical symptoms were observed in mice after CeNP administration
Cerium nanoparticles have special properties, especially low toxicity, unique capabilities in stimulating the immune system. Cerium nanoparticles can be considered an effective and safe candidate in vaccines.
How to Cite
2. Bouzigues C, Gacoin T, Alexandrou A. Biological applications of rare-earth based nanoparticles. ACS nano. 2011;5(11):8488-505.
3. Tsunekawa S, Sivamohan R, Ito S, Kasuya A, Fukuda T. Structural study on monosize CeO2-x nano-particles. Nanostructured materials. 1999;11(1):141-7.
4. Xia T, Kovochich M, Liong M, Madler L, Gilbert B, Shi H, et al. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS nano. 2008;2(10):2121-34.
5. Singh S, Kumar A, Karakoti A, Seal S, Self WT. Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles. Molecular BioSystems. 2010;6(10):1813-20.
6. D'Angelo B, Santucci S, Benedetti E, Di Loreto S, Phani R, Falone S, et al. Cerium oxide nanoparticles trigger neuronal survival in a human Alzheimer disease model by modulating BDNF pathway. Current Nanoscience. 2009;5(2):167-76.
7. Hijaz M, Das S, Mert I, Gupta A, Al-Wahab Z, Tebbe C, et al. Folic acid tagged nanoceria as a novel therapeutic agent in ovarian cancer. BMC cancer. 2016;16(1):1-14.
8. Sudo K, Yamada Y, Moriwaki H, Saito K, Seishima M. Lack of tumor necrosis factor receptor type 1 inhibits liver fibrosis induced by carbon tetrachloride in mice. Cytokine. 2005;29(5):236-44.
9. Marques Neto LM, Kipnis A, Junqueira-Kipnis AP. Role of metallic nanoparticles in vaccinology: implications for infectious disease vaccine development. Frontiers in immunology. 2017;8:239.
10. Chelliah M, Rayappan JBB, Krishnan UM. Synthesis and characterization of cerium oxide nanoparticles by hydroxide mediated approach. Journal of Applied Sciences. 2012;12(16):1734-7.
11. Nemati A, Assadollahi V, Peluso I, Abbaszadeh A, Beigi-Boroujeni M, Khanipur Z, et al. A stereological study of the toxic effects of cerium oxide during pregnancy on kidney tissues in neonatal NMRI mice. Oxidative Medicine and Cellular Longevity. 2020;2020.
12. Vishwakarma K, Vishwakarma O, Bhatele M, editors. A brief review on role of nanotechnology in medical sciences. Proceedings of All India Seminar on Biomedical Engineering 2012 (AISOBE 2012); 2013: Springer.
13. Jakupec M, Unfried P, Keppler B. Pharmacological properties of cerium compunds. Reviews of physiology, biochemistry and pharmacology. 2005:101-11.
14. Nikolova MP, Chavali MS. Metal oxide nanoparticles as biomedical materials. Biomimetics. 2020;5(2):27.
15. Sreekanth TVM, Nagajyothi PC, Reddy GR, Shim J, Yoo K. Urea assisted ceria nanocubes for efficient removal of malachite green organic dye from aqueous system. Scientific reports. 2019;9(1):1-9.
16. Nadeem M, Khan R, Afridi K, Nadhman A, Ullah S, Faisal S, et al. Green synthesis of cerium oxide nanoparticles (CeO2 NPs) and their antimicrobial applications: a review. International Journal of Nanomedicine. 2020;15:5951.
17. Cullity BD. Elements of X-ray Diffraction: Addison-Wesley Publishing; 1956.
18. Schubert D, Dargusch R, Raitano J, Chan S-W. Cerium and yttrium oxide nanoparticles are neuroprotective. Biochemical and biophysical research communications. 2006;342(1):86-91.
19. Soltani F, Yavari K, Sadeghi M, BAHRAMI SAMANI A, SHIRVANI ARANI S. Toxicity of nano and bulk forms of Cerium oxide in different cell lines. Iranian Journal of Pharmacology and Therapeutics. 2018;16(1):1-6.
20. Chen PL, Chen IW. Reactive cerium (IV) oxide powders by the homogeneous precipitation method. Journal of the American Ceramic Society. 1993;76(6):1577-83.
21. Darroudi M, Hakimi M, Sarani M, Oskuee RK, Zak AK, Gholami L. Facile synthesis, characterization, and evaluation of neurotoxicity effect of cerium oxide nanoparticles. Ceramics International. 2013;39(6):6917-21.
22. Arnold M, Badireddy A, Wiesner M, Di Giulio R, Meyer J. Cerium oxide nanoparticles are more toxic than equimolar bulk cerium oxide in Caenorhabditis elegans. Archives of environmental contamination and toxicology. 2013;65(2):224-33.
23. Ribeiro FM, de Oliveira MM, Singh S, Sakthivel TS, Neal CJ, Seal S, et al. Ceria Nanoparticles decrease UVA-induced fibroblast death through cell redox regulation leading to cell survival, migration and proliferation. Frontiers in Bioengineering and Biotechnology. 2020;8.
24. Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF. Metal‐based nanoparticles and their toxicity assessment. Wiley interdisciplinary reviews: Nanomedicine and Nanobiotechnology. 2010;2(5):544-68.
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