The Anti-Inflammatory and Antioxidant Activity of Aspirin in Septic Animal Models
International Journal of Medical Toxicology and Forensic Medicine,
Vol. 10 No. 3 (2020),
27 September 2020
,
Page 32550
https://doi.org/10.32598/ijmtfm.v10i3.32550
Abstract
Background: Sepsis is a systemic body reaction to invasive microorganisms, such as bacteria and fungi. Furthermore, it is one of the top ten main causes of death among all patients admitted to the hospital. Multiple potential drug therapies have been investigated in this area; however, an effective pharmacotherapy for sepsis remains undiscovered. Therefore, we explored the effect of Aspirin or Acetylsalicylic Acid (ASA) on the treatment outcomes and reduction of sepsis complications concerning the parameters involved in the oxidative damage of liver tissue. To perform an in vivo experiment, an experimental inflammatory model Cecal Ligation and Puncture (CLP) was performed in rats.
Methods: The investigated rats were divided into 4 groups (n=40), as follows: 1. Controls; 2. Laparotomy (LAP) group; 3. CLP group; and 4. The treatment group with aspirin 2 mg/kg bw for 48 h after CLP induction. Then, the explored rats were anesthetized and blood samples were collected from their hearts. Next, the animals were sacrificed and the liver tissue was separated for histopathologic and biochemical studies.
Results: The obtained data suggested that the treatment of animals with aspirin was effective in adjusting the antioxidant and inflammatory parameters. Pathological studies also indicated that sepsis led to injuries in the liver tissues, which could be improved by interventions.
Conclusion: In conclusion, sepsis caused oxidative damage in the liver tissue, and using aspirin was effective in preventing and improving these injuries.
- Cecal Ligation and Puncture (CLP), Oxidative stress, Gene expression, Aspirin, Sepsis
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References
Lee KH, Lee J, Lee SH. 3D liver models on a microplatform: well-defined culture, engineering of liver tissue and liver-on-a-chip. Lab Chip. 2015; 15(19):3822-37. [DOI:10.1039/C5LC00611B] [PMID]
Strnad P, Tacke F, Koch A, Trautwein C. Liver-guardian, modifier and target of sepsis. Nat Rev Gastroenterol Hepatol. 2017; 14(1):55-66. [DOI:10.1038/nrgastro.2016.168] [PMID]
Seymour CW, Liu VX, Iwashyna TJ, Brunkhorst FM, Rea TD, Scherag A, et al. Assessment of clinical criteria for sepsis: for the third International consensus definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016; 315(8):762-74. [DOI:10.1001/jama.2016.0288] [PMID] [PMCID]
Hubbard WJ, Choudhry M, Schwacha MG, Kerby JD, Rue LW, Bland KI, et al. Cecal ligation and puncture. Shock. 2005; 24(Suppl. 1):52-7. [DOI:10.1097/01.shk.0000191414.94461.7e] [PMID]
Ruiz S, Vardon-Bounes F, Merlet-Dupuy V, Conil JM, Buléon M, Fourcade O, Tack I, et al. Sepsis modeling in mice: Ligation length is a major severity factor in cecal ligation and puncture. Intensive Care Med Exp. 2016; 4(1):22. [DOI:10.1186/s40635-016-0096-z] [PMID] [PMCID]
Benjamim CF, Hogaboam CM, Kunkel SL. The chronic consequences of severe sepsis. J Leukoc Biol. 2004; 75:408-412. [DOI:10.1189/jlb.0503214] [PMID]
Legras A, Giraudeau B, Jonville-Bera A-P, Camus C, François B, Runge I, et al. A multicentre case-control study of nonsteroidal anti-inflammatory drugs as a risk factor for severe sepsis and septic shock. Crit Care. 2009; 13(2):R43. [DOI:10.1186/cc7766] [PMID] [PMCID]
Le Turnier P, Boutoille D, Joyau C, Veyrac G, Asseray N. Bacterial infections and NSAIDs exposure? Seek septic complications. Eur J Rep Intern Med. 2017; 41:e33-e34. [DOI:10.1016/j.ejim.2017.03.004] [PMID]
Floyd CN, Ferro A. Mechanisms of aspirin resistance. Pharmacol Ther. 2014; 141(1):69-78. [DOI:10.1016/j.pharmthera.2013.08.005] [PMID]
Rasooli A, Ghafari E, Saeidi H, Miri S. Expression changes of CD177 and MPO as novel biomarkers in lung tissue of CLP model rats. Turk J Med Sci. 2018; 48(6):1321-7. [DOI:10.3906/sag-1806-223] [PMID]
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978; 52:302-10. [DOI:10.1016/S0076-6879(78)52032-6]
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82: 70-77. [DOI:10.1016/0003-9861(59)90090-6]
Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: Estimation of neutrophil content with an enzyme marker. J Invest Dermatol. 1982; 78:206-9. [DOI:10.1111/1523-1747.ep12506462] [PMID]
Crimi E, Sica V, Slutsky AS, Zhang H, Williams-Ignarro S, Ignarro LJ Napoli C. Role of oxidative stress in experimental sepsis and multisystem organ dysfunction. Free Radic Res. 2006; 40:665-72. [DOI:10.1080/10715760600669612] [PMID]
Wheeler DS. Oxidative stress in critically Ill children with sepsis. Open Inflamm J. 2011; 4:(s1)74-81. [DOI:10.2174/1875041901104010074] [PMID] [PMCID]
Gelaim DP, de Bittencourt Pasqual MA, Comim CM, Grunwald MS, Ritter C, Damiani Tomasi C, et al. Serum heat-shock protein 70 levels, oxidant status, and mortality in sepsis. Shock. 2011; 35(5):466-70. [DOI:10.1097/SHK.0b013e31820fe704] [PMID]
El-Benna J, Hurtado-Nedelec M, Marzaioli V, Marie JC, Gougerot-Pocidalo MA, Dang PM. Priming of the neutrophil respiratory burst: Role in host defense and inflammation. Immunol Rev. 2016; 273(1):180-93. [DOI:10.1111/imr.12447] [PMID]
Jaganjac M, Cipak A, Schaur RJ, Zarkovic N. Pathophysiology of neutrophil-mediated extracellular redox reactions. Front Biosci (Landmark Ed). 2016; 21:839-55. [DOI:10.2741/4423] [PMID]
Alonso A, Misialek JR, Amiin MA, Hoogeveen RC, Chen LY, Agarwal SK, et al. Circulating levels of liver enzymes and incidence of atrial fibrillation: The atherosclerosis risk in communities cohort. Heart. 2014; 100(19):1511-6. [DOI:10.1136/heartjnl-2014-305756] [PMID] [PMCID]
Toscano MG, Ganea D, Gamero AM. Cecal ligation puncture procedure. J Vis Exp. 2011; 7(51):e2860. [DOI:10.3791/2860] [PMID] [PMCID]
Dadkhah A, Fatemi F, Mohammadi Malayeri MR, Rasooli A, Karvin Ashtiani MH. [The effects of Mentha Spicata on oxidative stress and COX-2 gene expression in prevention of sepsis (Persian)]. J Mol Cell Res. 2018; 31(4):484-99. http://cell.ijbio.ir/article_1421.html
Dadkhah A, Fatemi F, Rasooli A, Mohammadi Malayeri MR, Torabi F. Assessing the effect of Mentha longifolia essential oils on COX-2 expression in animal model of sepsis induced by caecal ligation and puncture. Pharm Biol. 2018; 56(1):495-504. [DOI:10.1080/13880209.2018.1510972] [PMID] [PMCID]
Zhao H, Luo F, Li H, Zhang L, Yi Y, Wan J. Antinociceptive effect of tetrandrine on LPS-induced hyperalgesia via the inhibition of IKKβ phosphorylation and the COX-2/PGE2 pathway in mice. PLoS One. 2014; 9(4):e94586. [DOI:10.1371/journal.pone.0094586] [PMID] [PMCID]
Halushka PV, Wise WC, Cook JA. Studies on the beneficial effects of aspirin in endotoxic shock. Relationship to inhibition of arachidonic acid metabolism. Am J Med. 1983; 74(6):91-6. [DOI:10.1016/0002-9343(83)90535-1]
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