Ultrasound-assisted liquid-liquid extraction for the determination of methadone in urine samples using gas chromatography-mass spectrometry
International Journal of Medical Toxicology and Forensic Medicine,
Vol. 10 No. 3 (2020),
27 September 2020
Background: Methadone abuse and dependence has been reported as a growing concern in some countries. In present study, a rapid, simple, easy and sensitive method for determination of methadone in human urine samples for use in clinical and forensic toxicology and drug screening laboratories was developed and validated.
Methods: We determined methadone in urine sampled by gas chromatography/mass spectrometry (GC/MS) and used ultrasound assisted liquid-liquid extraction (UALLE) method for the extraction and preconcentration of methadone before analysis.
Results: The limit of detection was 2.1 ng/mL and the limit of quantification 7 ng/mL. Correlation coefficient was 0.9984 for the methadone calibration curve in linear range from 7 to 10,000 ng/mL. The method is accurate and precise. Recovery was in the range of 81.3 % to 97.4 % and enrichment factor was 8.7. The method was successfully applied for determining methadone in clinical and postmortem urine samples.
Conclusions: The present method is a rapid, simple, easy and sensitive procedure and can be used in clinical and forensic toxicology laboratories as routine method for qualitative and quantitative analysis of methadone.
- Gas chromatography-Mass spectrometry (GC-MS)
- Ultrasound assisted liquid-liquid microextractio
How to Cite
Noble F, Marie N. Management of opioid addiction with opioid substitution treatments: beyond methadone and buprenorphine. Frontiers in Psychiatry. 2019;9:742. [DOI: 10.3389/fpsyt.2018.00742]
Brown R, Kraus C, Fleming M, Reddy S. Methadone: applied pharmacology and use as adjunctive treatment in chronic pain. Postgraduate Medical Journal. 2004;80(949):654-9. [DOI: 10.1136/pgmj.2004.022988]
Bell J, Zador D. A risk-benefit analysis of methadone maintenance treatment. Drug Safety. 2000; 22:179-90. [DOI:10.2165/00002018-200022030-00002]
Clausen T, Anchersen K, Waal H. Mortality prior to, during and after opioid maintenance treatment (OMT): A national prospective cross-registry study. Drug and Alcohol Dependence. 2008;94:151-7. [DOI:10.1016/j.drugalcdep.2007.11.003]
Soltaninejad K, Hassanian-Moghaddam H, Shadnia S. Methadone related poisoning on the rise in Tehran, Iran. Asia Pacific Journal of Medical Toxicology. 2014;3:104-9. [DOI: 10.22038/APJMT.2014.3378]
Soroosh D, Neamatshahi M, Zarmehri B, Nakhaee S, Mehrpour O. Drug-induced prolonged corrected QT interval in patients with methadone and opium overdose. Substance Abuse Treatment, Prevention and Policy. 2019;14(1):8. [DOI: 10.1186/s13011-019-0196-3]
Hadland SE, Levy S. Objective testing – urine and other drug tests.
Child and Adolescent Psychiatric Clinics of North America. 2016; 25(3): 549–565. [DOI:10.1016/j.chc.2016.02.005]
Sullivan HR, Blake DA. Quantitative determination of methadone concentrations in human blood, plasma and urine by gas chromatography. Research Communications in Chemical Pathology and Pharmacology. 1972;3(3):467-78. [PMID:5034508]
Myung SW, Kim S, Park JH, Kim M, Lee JC, Kim TJ. Solid-phase microextraction for the determination of pethidine and methadone in human urine using gas chromatography with nitrogen-phosphorus detection. Analyst. 1999;124(9):1283-6. [PMID:10736851]
Shamsipur M, Fattahi N. Extraction and determination of opium alkaloids in urine samples using dispersive liquid–liquid microextraction followed by high performance liquid chromatography. Journal of Chromatography B.2011;879(28):2978–83. [DOI:10.1016/j.jchromb.2011.08.033]
Rezaeepour R, Heydari R, Ismaili A. Ultrasound and salt-assisted liquid–liquid extraction as an efficient method for natural product extraction. Analytical Methods. 2015, 7: 3253-59. [DOI:10.1039/c5ay00150a]
Ridgway K, Lalljie SP, Smith RM. Sample preparation techniques for the determination of trace residues and contaminants in foods. Journal of Chromatography A. 2007; 1153(1-2):36-53. [DOI:10.1016/j.chroma.2007.01.134]
Pavlovic´ DM, Babic´ S, Horvat AJM, Kasˇtelan-Macan M. Sample preparation in analysis of pharmaceuticals. Trends in Analytical Chemistry. 2007; 26(11):1062 -65. [DOI:10.1016/j.trac.2007.09.010]
Sushma DD, Jaimala JA, Priya BP. Ultrasonication: A unique extraction method of crude drugs. Joinsysmed. 2015; 3(4):203-205.
Cheng PS, Lee CH, Liu C, Chien CS. Simultaneous determination of ketamine, tramadol, methadone, and their metabolites in urine by gas chromatography-mass spectrometry. Journal of Analytical Toxicology. 2008;32(3):253-9. [DOI:10.1093/jat/32.3.253]
Alahyari E, Setareh, M, Shekari A, Roozbehani G, Soltaninejad K. Analysis of opioids in postmortem urine samples by dispersive liquid-liquid microextraction and high performance liquid chromatography with photo diode array detection. Egyptian Journal of Forensic Science. 2018;8:13. [DOI: 10.1186/s41935-018-0046-x]
Bratinčević MV, Visković T, Sutlović D. Comparison of the solid phase and liquid-liquid extraction methods for methadone determinationin human serum and whole blood samples using gas chromatography/mass spectrometry. Archives of Industrial Hygiene and Toxicology. 2017;68(4):308-314. [DOI: 10.1515/aiht-2017-68-2953]
SWGTOX. Scientific working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. 2013. Available from: https://academic.oup.com/jat/article/37/7/452/765476, 31 May 2019
Ranjbari E, Golbabanezhad-Azizi AA, Hadjmohammadi MR. Preconcentration of trace amounts of methadone in human urine, plasma, saliva and sweat samples using dispersive liquid-liquid microextraction followed by high performance liquid chromatography. Talanta. 2012;94:116-22. [DOI: 10.1016/j.talanta.2012.03.004]
Lin Z, Li J, Zhang X, Qiu M, Huang Z, Rao Y. Ultrasound-assisted dispersive liquid-liquid microextraction for the determination of seven recreational drugs in human whole blood using gas chromatography-mass spectrometry. Journal of Chromatography B. 2017;1046:177-184. [DOI: 10.1016/j.jchromb.2017.01.024]
Meng L, Zhang W, Meng P, Zhu B, Zheng K. Comparison of hollow fiber liquid-phase microextraction and ultrasound-assisted low-density solvent dispersive liquid-liquid microextraction for the determination of drugs of abuse in biological samples by gas chromatography-mass spectrometry. Journal of Chromatography B. 2015;989:46-53. [DOI: 10.1016/j.jchromb.2015.02.039]
Zhong Z, Li G, Zhong X, Luo Z, Zhu B. Ultrasound-assisted low-density solvent dispersive liquid–liquid extraction for the determination of alkanolamines and alkylamines in cosmetics with ion chromatography. Talanta. 2013; 115:518–25. [DOI: 10.1016/j.talanta.2013.04.045]
Piao C, Chen L, Wang Y. A review of the extraction and chromatographic determination methods for the analysis of parabens. Journal of Chromatography B. 2014;969: 139–48. [DOI: 10.1016/j.jchromb.2014.08.015]
Chen PS, Haung WY, Huang SD. Analysis of triazine herbicides using an up-and-down-shaker-assisted dispersive liquid-liquid microextractioncoupled with gas chromatography-mass spectrometry. Journal of Chromatography B. 2014;955–956: 116–23. [DOI: 10.1016/j.jchromb.2014.02.032]
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