Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts
Iranian Endodontic Journal,
Vol. 10 No. 2 (2015),
1 March 2015
,
Page 89-92
https://doi.org/10.22037/iej.v10i2.8485
Abstract
Introduction: Assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. In a recent study, 5% fluoride varnish (Duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. The aim of the present study was to compare cytotoxicity of Duraflur varnish with two popular commonly used root canal sealers (AH-Plus and AH-26) on human gingival fibroblasts (HGF). Methods and Materials: The HGFs were incubated with different concentrations (1/2, 1/4, and 1/8) of AH-plus, AH-26, and Duraflur varnish for 24 h. The percentage of cell viability was assessed with methyl-thiazol-tetrazolium (MTT) assay. The data was analyzed using the one-way ANOVA followed by Student-Newman-Keuls test. The level of significance was set at 0.001. Results: MTT assay showed that higher concentrations of the tested materials resulted in lower viability of HGFs. AH-Plus showed significantly greater cell viability compared to AH-26 at all dilutions (P<0.001); however, no significant difference was found between Duraflur and AH-Plus in terms of cell viability at 1/8 dilution (P>0.001). Duraflur showed significantly higher cell viability compared to AH-26 except at 1/2 dilution (P<0.001). Conclusion: Although Duraflur varnish had better biocompatibility compared to AH-26, it should still be evaluated with further biocompatibility tests such as intraosseous and subcutaneous implantation.How to Cite
1.
Forghani FR, Parirokh M, Paseban H, Asgary S, Askarifard S, Esmaeeli Mahani S. Cytotoxicity of Two Resin-Based Sealers and a Fluoride Varnish on Human Gingival Fibroblasts. Iran Endod J [Internet]. 2015 Mar. 18 [cited 2024 Apr. 19];10(2):89-92. Available from: https://journals.sbmu.ac.ir/iej/article/view/8485
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