Effect of Biotitania and Titania Addition on Bioactivity and Antibacterial Properties of Calcium Silicate Cement
Iranian Endodontic Journal,
Vol. 15 No. 3 (2020),
1 July 2020
Introduction: Nanoparticles are gaining more interest in dentistry for their antimicrobial, physical as well as other properties. This study aimed to evaluate the effect of adding two types of nanoparticles (NPs) on calcium silicate hydraulic cement’s (CSHC) unique bioactivity and antibacterial properties. Methods and Materials: Biotitania/AgCl NPs were synthetized and characterized for its morphology, types of formed functional groups and crystalline AgCl using field emission scanning electron microscope (FE-SEM) equipped with energy-dispersive X-ray spectroscopy (EDS), X-ray diffractometer (XRD), Fourier transformation infrared spectroscopy (FT-IR) and thermo-gravimetric analysis (TGA). The former NPs and commercial titania (TiO2) NPs were added (0.5, 1.5 and 3-weight %) to commercial CSHS powder. A total of 140 disk-shaped specimens (10 mm×1 mm) were prepared (seven material groups per each test in addition to the eighth cell control group) to evaluate cell viability and alkaline phosphatase activity (ALP) after 3 and 12 days, respectively. All were incubated with mesenchymal stem cells. Antibacterial efficacy against Streptococcus mutans (S. mutans) was evaluated through the bacterial growth curve slopes while being in direct contact with the tested material groups for 18 h. Results: Addition of all NPs percentages had no significant effect (P>0.05) on cell viability in comparison to positive control CSHC. Commercial TiO2 NPs (0.5 weight %) had statistically significant lower values (P≤0.05) for bacterial growth curve slope. However, addition of all NPs percentages had significantly improved (P≤0.05) the ALP activity of CSHC with the most prominent effect to 3-weight% biotitania/AgCl NPs. Conclusion: Based on this in vitro study, addition of biotitania/AgCl NPs up to 3-weight% significantly improved the bioactivity of CSHC without having a significant negative impact on its antibacterial efficacy. Interestingly, the addition of commercial TiO2 even in small amounts can significantly improve CSHC antibacterial efficacy.
- Antibacterial Efficacy; Bioactivity; Biotitania; Calcium Silicate Cements; Silver Chloride; Titania
How to Cite
Feng D, Xie N, Gong C, Leng Z, Xiao H, Li H. Portland Cement Paste Modified by TiO2 Nanoparticles : A Microstructure Portland Cement Paste Modified by TiO2 Nanoparticles : A Microstructure Perspective 2013;52(33):11575-82.
Maria J, Tanomaru G, Storto I, Ferreira G, Silva DA, Bosso R, et al. Radiopacity , pH and antimicrobial activity of Portland cement associated with micro- and nanoparticles of zirconium oxide and niobium oxide. Dent Mater J 2014;33(4):466–70.
Samiei M, Ghasemi N, Aghazadeh M, Divband B, Akbarzadeh F. Biocompatibility of Mineral Trioxide Aggregate with TiO 2 Nanoparticles on Human Gingival Fibroblasts. J Clin Exp Dent 2017;9(2):e182-e5.
Samiei M, Janani M, Asl-aminabadi N, Ghasemi N, Divband B. Effect of the TiO2 nanoparticles on the selected physical properties of mineral trioxide aggregate. 2017;9(2):e191-e5.
Simila HO, Karpukhina N, Hill RG. Bioactivity and fluoride release of strontium and fluoride modified Biodentine. Dent Mater 2017;34(1):e1–7.
Septodent. Biodentine scientific file. 2010;(01). Available from: https://www.septodontusa.com/sites/default/files/2016-07/Biodentine-Case-Studies.pdf
Pawar A, Kokate S, Shah R. Management of a large periapical lesion using Biodentine TM as retrograde restoration with eighteen months evident follow up. J Conserv Dent 2013;16(6):573-5.
Hiremath G, Kulkarni R, Naik B. Evaluation of minimal inhibitory concentration of two new materials using tube dilution method: An in vitro study. J Conserv Dent 2015;18(2):159-62.
Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials. Dent Mater 2013;29(2):e20-8.
Caron G, Azérad J, Faure MO, Machtou P, Boucher Y. Use of a new retrograde filling material (Biodentine) for endodontic surgery: Two case reports. Int J Oral Sci 2014;6(4):250–3.
Ravichandra P V., Harikumar V, Deepthi K, Jayaprada RS, Ramkiran D, Krishna M. JN, et al. Comparative evaluation of marginal adaptation of biodentineTM and other commonly used root end filling materials-an invitro study. J Clin Diagnostic Res 2014;8(3):243–5.
Kaup M, Schäfer E, Dammaschke T. An in vitro study of different material properties of Biodentine compared to ProRoot MTA. Head Face Med. 2015;11(1):13–5.
Standardization FOR, Normalisation DE. International Standard Iso. 1987.
Hashem DF, Foxton R, Manoharan A, Watson TF, Banerjee A. The physical characteristics of resin composite-calcium silicate interface as part of a layered/laminate adhesive restoration. Dent Mater 2014;30(3):343–9.
Mendes MSS, Resende LD, Pinto CA, Raldi DP, Cardoso FGR, Habitante SM. Radiopacity of mineral trioxide aggregate with and without inclusion of silver nanoparticles. J Contemp Dent Pract 2017;18(6):448–51.
Mestieri LB, Gomes-Cornélio AL, Rodrigues EM, Faria G, Guerreiro-Tanomaru JM, Tanomaru-Filho M. Cytotoxicity and bioactivity of calcium silicate cements combined with niobium oxide in different cell lines. Braz Dent J 2017;28(1):65–71.
Vazquez-Garcia F, Tanomaru-Filho M, Chávez-Andrade GM, Bosso-Martelo R, Basso-Bernardi MI, Guerreiro-Tanomaru JM. Effect of silver nanoparticles on physicochemical and antibacterial properties of calcium silicate cements. Braz Dent J 2016;27(5):508–14.
Prentice LH, Tyas MJ, Burrow MF. The effect of ytterbium fluoride and barium sulphate nanoparticles on the reactivity and strength of a glass-ionomer cement. Dent Mater 2006;22(8):746–51.
Bischoff BL, Anderson MA. Peptization Process in the Sol-Gel Preparation of Porous Anatase (TiO2). Chem Mater 1995;7(10):1772–8.
Yang J, Mei S. Hydrothermal Synthesis of Nanosized Titania Powders : Influence of Tetraalkyl Ammonium Hydroxides on Particle Characteristics. J Am Ceram Soc 2001;702(8):1696–702.
Elsaka SE, Hamouda IM, Swain M V. Titanium dioxide nanoparticles addition to a conventional glass-ionomer restorative: Influence on physical and antibacterial properties. J Dent 2011;39(9):589–98.
Abdul Jalill RD, Nuaman RS, Abd AN. Biological synthesis of Titanium Dioxide nanoparticles by Curcuma longa plant extract and study its biological properties. WSN 2016;49(2):204–22.
Jha Z, Behar N, Sharma S, Chandel G, Sharma D, Pandey M. Nanotechnology: Prospects of Agricultural Advancement. Nano Vis 2011;1(2):88–100.
Zhou T, Huang Y, Li W, Cai Z, Luo F, Yang CJ, et al. Facile synthesis of red-emitting lysozyme-stabilized Ag nanoclusters. Nanoscale 2012;4(17):5312–5.
Luckarift HR, Dickerson MB, Sandhage KH, Spain JC. Rapid, room-temperature synthesis of antibacterial bionanocomposites of lysozyme with amorphous silica or titania. Small 2006;2(5):640–3.
Fathy SM, Elkhooly TA, Emam AA, Reicha FM. Evaluation of naturally derived hydroxyapatite tissue engineering scaffold coated with chitosan- carbon nanotubes composite. EDJ 2019:65:91-102.
Fathy SM, Abd El-Aziz AM, Lahah DA. Cellular interaction and antibacterial efficacy of two hydraulic calcium silicate-based cements : cell-dependent model. J Conserv Dent 2019;22(1):17-22.
Bouhekka A, Bürgi T. In situ ATR-IR spectroscopy study of adsorbed protein: Visible light denaturation of bovine serum albumin on TiO2. Appl Surf Sci 2012;261:369–74.
Naik B, Ghosh NN, Desai V, Kowshik M, Prasad VS, Fernando GF. Synthesis of Ag/AgCl-mesoporous silica nanocomposites using a simple aqueous solution-based chemical method and a study of their antibacterial activity on E. coli. Particuology 2011;9(3):243–7.
Alrahlah A, Fouad H, Hashem M, Niazy AA, AlBadah A. Titanium Oxide (TiO2)/polymethylmethacrylate (PMMA) denture base nanocomposites: Mechanical, viscoelastic and antibacterial behavior. Materials (Basel). 2018;11(7):1096.
Fathima JB, Pugazhendhi A, Venis R. Synthesis and characterization of ZrO2 nanoparticles-antimicrobial activity and their prospective role in dental care. Microb Pathog 2017;110(2):245–51.
Samiei M, Ghasemi N, Aghazadeh M, Divband B, Akbarzadeh F. Biocompatibility of mineral trioxide aggregate with TiO2 nanoparticles on human gingival fibroblasts. J Clin Exp Dent 2017;9(2):e182–5.
Li X, Zuo W, Luo M, Shi Z, Cui Z, Zhu S. Silver chloride loaded hollow mesoporous aluminosilica spheres and their application in antibacterial coatings. Mater Lett 2013;105(10231):159–61.
Min SH, Yang JH, Kim JY, Kwon YU. Development of white antibacterial pigment based on silver chloride nanoparticles and mesoporous silica and its polymer composite. Microporous Mesoporous Mater 2010;128(1–3):19–25.
Kalishwaralal K, Deepak V, Ram Kumar Pandian SB, Kottaisamy M, BarathManiKanth S, Kartikeyan B, et al. Biosynthesis of silver and gold nanoparticles using Brevibacterium casei. Colloids Surfaces B Biointerfaces 2010;77(2):257–62.
Xiangqian L, Huizhong X, Zhe-Sheng C, Guofang C. Biosynthesis of Nanoparticles by Microorganisms and Their Applications. J Nanomater 2011:16 pages.
Sodagar A, Akhoundi MSA, Bahador A, Jalali YF, Behzadi Z, Elhaminejad F,
Mirhashemi AH. Effect of TiO2 nanoparticles incorporation on antibacterial
properties and shear bond strength of dental composite used in Orthodontics.
Dent Press J Orthod 2017; http://dx.doi.org/10.1590/2177-6709.22.5.067-074.oar
Garcia-Contreras R, Scougall-Vilchis RJ, Contreras-Bulnes R, Sakagami H, Morales-
Luckie RA, Nakajima H. Mechanical, antibacterial and bond strength properties of
nano-titanium-enriched glass ionomer cement. J Appl Oral Sci. 2015 May-
Kang YO, Jung J, Cho D, Kwon OH, Cheon JY, Park WH. Antimicrobial Silver
Chloride Nanoparticles Stabilized with Chitosan Oligomer for the Healing of Burns.
Materials (Basel) 2016;9(4):215.
Ghasemi N, Rahimi S, Lotfi M, Solaimanirad J, Shahi S, Shafaie H, et al. Effect of Mineral Trioxide Aggregate , Calcium-Enriched Mixture Cement and Mineral Trioxide Aggregate with Disodium Hydrogen Phosphate on BMP-2 Production. Iran Endo J 2014;9(3):220–4.
Ej S, Pm S, Aa Z. Cytocompatibility of Biodentine using a three-dimensional cell
culture model . Int Endod J 2016 Jun;49(6):574-80.
Sabetrasekh R, Tiainen H, Lyngstadaas SP, Reseland J, Haugen H. A novel ultra-porous titanium dioxide ceramic with excellent biocompatibility. J Biomater Appl 2011;25(6):559–80.
Kim JR, Nosrat A, Fouad AF. Interfacial characteristics of Biodentine and MTA with dentine in simulated body fluid. J Dent 2015;43(2):241–7.
Kaur M, Singh H, Dhillon JS, Batra M, Saini M. MTA versus Biodentine : Review of Literature with a Comparative Analysis. J Clin Diagn Res 2017;11(8):3–7.
Hoshyari N, Labbaf H, Naderi NJ, Kazemi A, Bastami F, Koopaei M. Biocompatibility of Portland Cement Modified with Titanium Oxide and Calcium Chloride in a Rat Model. Iran Endo J 2016;11(2):124–8.
Sengottuvelan A, Balasubramanian P, Will J, Boccaccini AR. Bioactive Materials Bioactivation of titanium dioxide scaffolds by ALP-functionalization. Bioact Mater 2017;2(2):108–15.
Mizuno M, Kuboki Y. Osteoblast-related gene expression of bone marrow cells during the osteoblastic differentiation induced by type I collagen. J Biochem 2001;129(1):133–8.
Hoemann CD, El-Gabalawy H, McKee MD. In vitro osteogenesis assays: Influence of the primary cell source on alkaline phosphatase activity and mineralization. Pathol Biol 2009;57(4):318–23.
Lee BN, Lee KN, Koh JT, Min KS, Chang HS, Hwang IN, et al. Effects of 3 endodontic bioactive cements on osteogenic differentiation in mesenchymal stem cells. J Endod 2014;40(8):1217–22.
Hou Y, Cai K, Li J, Chen X, Lai M, Hu Y, et al. Effects of titanium nanoparticles on adhesion, migration, proliferation, and differentiation of mesenchymal stem cells. Int J Nanomedicine 2013;8:3619–30.
Chen C-T, Shih Y-R V., Kuo TK, Lee OK, Wei Y-H. Coordinated Changes of Mitochondrial Biogenesis and Antioxidant Enzymes During Osteogenic Differentiation of Human Mesenchymal Stem Cells. Stem Cells 2008;26(4):960–8.
Mody N, Parhami F, Sarafian TA, Demer LL. Oxidative stress modulates osteoblastic differentiation of vascular and bone cells. Free Radic Biol Med 2001;31(4):509–19.
Qin H, Zhu C, An Z, Jiang Y, Zhao Y, Wang J, et al. Silver nanoparticles promote osteogenic differentiation of human urine-derived stem cells at noncytotoxic concentrations. Int J Nanomedicine 2014;9(1):2469–78.
Zhang R, Lee P, Lui VCH, Chen Y, Liu X, Lok CN, et al. Silver nanoparticles promote osteogenesis of mesenchymal stem cells and improve bone fracture healing in osteogenesis mechanism mouse model. Nanomedicine Nanotechnology, Biol Med 2015;11(8):1949–59.
Zhang S, Du C, Wang Z, Han X, Zhang K, Liu L. Reduced cytotoxicity of silver ions to mammalian cells at high concentration due to the formation of silver chloride. Toxicol Vitr 2013;27(2):739–44.
- Abstract Viewed: 106 times
- PDF Downloaded: 63 times