Current status of intestinal parasitic infections and associated risk factors in rural population of Guilan province, northern Iran: trichostrongyliasis is the most prevalent helminthic infection
Gastroenterology and Hepatology from Bed to Bench,
Vol. 17 No. 2 (2024),
14 April 2024
https://doi.org/10.22037/ghfbb.v17i2.2893
Abstract
Intestinal parasitic infections are still considered as a major public health concern, particularly in human communities with poor economy and sanitation. This study aimed to determine the distribution of enteric parasitic infections and related risk factors among rural communities of Guilan province, Northern Iran, and to compare the results with the situation in the past. This cross-sectional study was performed in rural areas of Masal and Shanderman district from February 2020 to December 202. A total of 917 stool samples were collected and examined for presence of intestinal helminthes and protozoa using direct, formalin-ether and Kato-Katz techniques. A total of 156 (17%) out of 917 examined individuals were infected with intestinal parasites. The overall prevalence of protozoa, helminths and mixed infections were 11.8% (108/917), 4.5% (41/917) and 0.8% (7/917), respectively. Blastocystis was the most prevalent intestinal protozoa (9.6%) followed by Giardia lamblia (1.9%), Endolimax nana (1.1%), E. coli (0.8%) and E. hartmani (0.1%). The highest prevalence of intestinal helminths belonged to Trichostongylus spp. (3.5%) and Strongyloides stercoralis (1.3%). Statistical analysis showed significant association between giardiasis and sex (P < 0.03). On the other hand, prevalence of enteric helminths was influenced by close contact with livestock, keeping herbivorous animals at home, job, education, and consumption of uncooked vegetables (P <0.05). The findings indicate a decreasing trend in the prevalence of intestinal parasitic infections in Guilan province in comparison to the past few decades. Hookworm infections, which was very prevalent in the area, are now rare, while trichostrongylosis showed a high prevalence in rural residents of the study area.
- Intestinal parasites
- Trichostrongyliasis
- Risk factors
- Guilan
- Iran
How to Cite
References
Brooker S (2010) Estimating the global distribution and disease burden of intestinal nematode infections: adding up the numbers–a review. Int J Parasitol 40 (10):1137-1144
Pullan RL, Smith JL, Jasrasaria R, Brooker SJ (2014) Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasite Vector 7:1-19
Hailegebriel T (2017) Prevalence of intestinal parasitic infections and associated risk factors among students at Dona Berber primary school, Bahir Dar, Ethiopia. BMC Infect Dis 17 (1):1-8
Silva JVLd, Fontes G, Santos CDd, Santos RVd, da Rocha EMM (2016) Factors associated with gastrointestinal parasitic infections among young population in Northeast Brazil. Canadian J Infect Dis Med Microbiol 2016
Lim Y, Romano N, Colin N, Chow S, Smith H (2009) Intestinal parasitic infections amongst Orang Asli (indigenous) in Malaysia: has socioeconomic development alleviated the problem? Trop Biomed 26 (2):110-122
Oberhelman RA, Guerrero ES, Fernandez ML, Silio M, Mercado D, Comiskey N, Ihenacho G, Mera R (1998) Correlations between intestinal parasitosis, physical growth, and psychomotor development among infants and children from rural Nicaragua. The American journal of tropical medicine and hygiene 58 (4):470-475
Patel J, Gupta D, Rathi C, Parikh P, Ingle M, Sawant P (2015) Uncommon presentation of Strongyloidiasis: chronic malabsorption, multiple small bowel strictures and appendicitis in HTLV-1 positive patient. Trop Gastroenterol 36:212-215
Liao C-W, Chiu K-C, Chiang I-C, Cheng P-C, Chuang T-W, Kuo J-H, Tu Y-H, Fan C-K (2017) Prevalence and risk factors for intestinal parasitic infection in schoolchildren in Battambang, Cambodia. The Am J Trop Med Hyg 96 (3):583
Gyang VP, Chuang T-W, Liao C-W, Lee Y-L, Akinwale OP, Orok A, Ajibaye O, Babasola AJ, Cheng P-C, Chou C-M (2019) Intestinal parasitic infections: current status and associated risk factors among school aged children in an archetypal African urban slum in Nigeria. J Microbiol Immunol Infect 52 (1):106-113
Forson AO, Arthur I, Olu-Taiwo M, Glover KK, Pappoe-Ashong PJ, Ayeh-Kumi PF (2017) Intestinal parasitic infections and risk factors: a cross-sectional survey of some school children in a suburb in Accra, Ghana. BMC Res Note 10:1-5
Rokni M (2008) The present status of human helminthic diseases in Iran. Annal Trop Med Parasitol 102 (4):283-295
Sharifdini M, Derakhshani S, Alizadeh SA, Ghanbarzadeh L, Mirjalali H, Mobedi I, Saraei M (2017) Molecular identification and phylogenetic analysis of human Trichostrongylus species from an endemic area of Iran. Act Trop 176:293-299
Sharifdini M, Ghanbarzadeh L, Barikani A, Saraei M (2020) Prevalence of intestinal parasites among rural inhabitants of Fouman, Guilan Province, Northern Iran with emphasis on Strongyloides stercoralis. Iran J Parasitol 15 (1):91-100
Sharifdini M, Ghanbarzadeh L, Kouhestani-Maklavani N, Mirjalali H, Saraei M (2017) Prevalence and molecular aspects of human hookworms in Guilan province, northern Iran. Iran J Parasitol 12 (3):374
Sabbaghian H, Arfaa F (1970) Prevalence and intensity of intestinal helminthiases in northwest and northeast of Iran. Act Med Iran:11-18
Arfaa F (1972) Present status of human helminthiasis in Iran. Trop Geographic Med 24 (4):353-362
Ghadirian E, Arfaa F, Missaghian G, Bahrani H (1973) Prevalence of intestinal helminthiasis in Kermanshah City and some villages in the province. Iran J Public Health 2:90-101
Alemi A, Arfaa F (1978) Prevalence of intestinal helminthiasis in the rural area of gilan province (caspian littoral). Iran J Public Health 7 (1):25-34
Sayyari A, Imanzadeh F, Bagheri Yazdi S, Karami H, Yaghoobi M (2005) Prevalence of intestinal parasitic infections in the Islamic Republic of Iran. EMHJ-East Mediterranean Health J, 11 (3), 377-383, 2005
Taheri F, Namakin K, Zarban A, Sharifzadeh G (2011). Intestinal parasitic infection among school children in South Khorasan province, Iran. J Res Health Sci, 11(1):45-50
Masoumeh R, Farideh T, Mitra S, Heshmatollah T (2012) Intestinal parasitic infection among school children in Golestan province, Iran. Pak J Biol Sci 15 (23):1119-1125
Daryani A, Sharif M, Nasrolahei M, Khalilian A, Mohammadi A, Barzegar G (2012) Epidemiological survey of the prevalence of intestinal parasites among schoolchildren in Sari, northern Iran. Trans Royal Soc Trop Med Hyg 106 (8):455-459
Gholipoor Z, Khazan H, Azargashb E, Youssefi MR, Rostami A (2020) Prevalence and risk factors of intestinal parasite infections in Mazandaran province, North of Iran. Clin Epidemiol Global Health 8 (1):17-20
Mahni MB, Rezaeian M, Kia EB, Raeisi A, Khanaliha K, Tarighi F, Kamranrashani B (2016) Prevalence of intestinal parasitic infections in Jiroft, Kerman Province, Iran. Iran J Parasitol 11 (2):232
Kazemi E, Rostamkhani P, Hooshyar H (2017) A survey on prevalence of intestinal parasites infections in patients referred to the public hospital in Khoy, West Azarbaijan province, Iran, 2014-2016. Avicenna J Clin Microbiol Infect 4 (4):56114-56114
Beiromvand M, Panabad E, Rafiei A (2019) Status of intestinal parasitic infections among rural and urban populations, southwestern Iran. Asian Pacific J Trop Med 12 (3):130
Jafari R, Fallah M, Darani HY, Yousefi HA, Mohaghegh MA, Latifi M, Sadaghian M, Maghsood AH (2014) Prevalence of intestinal parasitic infections among rural inhabitants of Hamadan city, Iran, 2012. Avicenna J Clin Microbiol Infect 1 (2)
Pandi M, Sharifdini M, Ashrafi K, Atrkar Roushan Z, Rahmati B, Hajipour N (2021) Comparison of molecular and parasitological methods for diagnosis of human trichostrongylosis. Front Cell Infect Microbiol:959
Souza RP, Souza JN, Menezes JF, Alcântara LM, Soares NM, Aquino Teixeira MC (2013) Human infection by Trichostrongylus spp. in residents of urban areas of Salvador city, Bahia, Brazil. Biomédica 33 (3):439-445
Magambo J, Zeyhle E, Wachira T (1998) Prevalence of intestinal parasites among children in southern Sudan. East African Med J 75 (5):288-290
Watthanakulpanich D, Pongvongsa T, Sanguankiat S, Nuamtanong S, Maipanich W, Yoonuan T, Phuphisut O, Boupha B, Moji K, Sato M (2013) Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR. Act Trop 126 (1):37-42
Ghadirian E, Arfaa F (1975) Present status of trichostrongyliasis in Iran. Am J Trop Med Hyg 24 (6 Pt 1):935-941
Ashrafi K, Tahbaz A, Sharifdini M, Mas-Coma S (2015) Familial Trichostrongylus infection misdiagnosed as acute fascioliasis. Emerg Infect Dis 21 (10):1869
Mowlavi G, MirAhmadi H, Rezaeian M, Kia E, Rokni M, Golestan B, Shafiei R, Fereshtehnejad S, Keramati M (2008) Prevalence of intestinal parasites in tribal parts of Khuzestan Province during 2005-07. Govaresh 12 (4):219-228
Barbosa CV, Barreto MM, Andrade RdJ, Sodré F, d’Avila-Levy CM, Peralta JM, Igreja RP, de Macedo HW, Santos HLC (2018) Intestinal parasite infections in a rural community of Rio de Janeiro (Brazil): prevalence and genetic diversity of Blastocystis subtypes. PLoS One 13 (3):e0193860
Santos AP, Fernández FN, Sánchez RM, Cañete ID, Rodríguez M, Puebla LJ, González ZR (2014) Prevalence and risk factors for intestinal parasitic infections in a rural community in “Consolación del Sur” municipality, Cuba. West Indian Med J 63 (4):333
Rop DC, Nyanchongi BO, Nyangeri J, Orucho VO (2016) Risk factors associated with intestinal parasitic infections among inmates of Kisii prison, Kisii county, Kenya. BMC Res Note 9 (1):1-10
Punsawad C, Phasuk N, Bunratsami S, Thongtup K, Siripakonuaong N, Nongnaul S (2017) Prevalence of intestinal parasitic infection and associated risk factors among village health volunteers in rural communities of southern Thailand. BMC Public Health 17 (1):1-9
Khieu V, Schär F, Marti H, Bless PJ, Char MC, Muth S, Odermatt P (2014) Prevalence and risk factors of Strongyloides stercoralis in Takeo province, Cambodia. Parasite Vectors 7:1-8
Khieu V, Schär F, Forrer A, Hattendorf J, Marti H, Duong S, Vounatsou P, Muth S, Odermatt P (2014) High prevalence and spatial distribution of Strongyloides stercoralis in rural Cambodia. PLoS Negl Trop Dis 8 (6):e2854
Forrer A, Khieu V, Schär F, Vounatsou P, Chammartin F, Marti H, Muth S, Odermatt P (2018) Strongyloides stercoralis and hookworm co-infection: spatial distribution and determinants in Preah Vihear province, Cambodia. Parasite Vectors 11:1-13
Steinmann P, Zhou X-N, Du Z-W, Jiang J-Y, Wang L-B, Wang X-Z, Li L-H, Marti H, Utzinger J (2007) Occurrence of Strongyloides stercoralis in Yunnan Province, China, and comparison of diagnostic methods. PLoS Negl Trop Dis 1 (1):e75
Suntaravitun P, Dokmaikaw A (2018) Prevalence of intestinal parasites and associated risk factors for infection among rural communities of Chachoengsao Province, Thailand. Korean J Parasitol 56 (1):33
Naves MM, Costa-Cruz JM (2013) High prevalence of Strongyloides stercoralis infection among the elderly in Brazil. Rev Instit Med Trop São Paulo 55:309-313
Amor A, Rodriguez E, Saugar JM, Arroyo A, López-Quintana B, Abera B, Yimer M, Yizengaw E, Zewdie D, Ayehubizu Z (2016) High prevalence of Strongyloides stercoralis in school-aged children in a rural highland of north-western Ethiopia: the role of intensive diagnostic work-up. Parasite Vectors 9 (1):1-8
Jaleta TG, Zhou S, Bemm FM, Schär F, Khieu V, Muth S, Odermatt P, Lok JB, Streit A (2017) Different but overlapping populations of Strongyloides stercoralis in dogs and humans—Dogs as a possible source for zoonotic strongyloidiasis. PLoS Neglected Tropical Diseases 11 (8):e0005752
Nagayasu E, Aung MPPTHH, Hortiwakul T, Hino A, Tanaka T, Higashiarakawa M, Olia A, Taniguchi T, Win SMT, Ohashi I (2017) A possible origin population of pathogenic intestinal nematodes, Strongyloides stercoralis, unveiled by molecular phylogeny. Sci Rep 7 (1):4844
Viesy S, Abdi J, Rezaei Z (2019) What are hidden facts behind intestinal parasitic infections in Ilam city? Infect Disorder Drug Targ (Formerly Current Drug Targets-Infectious Disorders) 19 (3):284-287
Hemmati N, Razmjou E, Hashemi-Hafshejani S, Motevalian A, Akhlaghi L, Meamar AR (2017) Prevalence and risk factors of human intestinal parasites in Roudehen, Tehran province, Iran. Iran J Parasitol 12 (3):364
Sadeghi H, Borji H (2015) A survey of intestinal parasites in a population in Qazvin, north of Iran. Asian Pac J Trop Dis 5 (3):231-233
Pestehchian N, Nazari M, Haghighi A, Salehi M, Yosefi HA, Khosravi N (2015) Prevalence of intestinal parasitic infection among inhabitants and tribes of Chelgerd, Iran, 2008-2009. J Clin Diag Res: JCDR 9 (5):LC01
Shahnazi M, Abdollahpour H, Alipour M, Sadeghi M, Saraei M, Hajialilo E, Heydarian P (2019) Prevalence of intestinal parasites in food handlers of the city of Saqqez in 2016. J Parasit Dis 43:113-119
Sedighi I, Asadi M, Olfat M, Maghsood AH (2015) Prevalence and risk factors of Giardia lamblia and Blastocystis hominis infections in children under ten years old, Hamadan, Iran. Avicenna J Clin Microbiol Infect 2 (2):22713-22713
Sarkari B, Hosseini G, Motazedian MH, Fararouei M, Moshfe A (2016) Prevalence and risk factors of intestinal protozoan infections: a population-based study in rural areas of Boyer-Ahmad district, Southwestern Iran. BMC infectious diseases 16:1-5
Al-Naemy BS, Al-Kalak S, Rahemo ZI (2012) The intestinal parasites of Bashiqa district, Nineveh governorate, Iraq. Int J Mol Zool 2
Jarallah HM (2012) Intestinal parasitic infections among rural villages in Basrah marshes regions. J Basrah Res Sci 2012; 38 (2): 40 43
Al-Hamairy, AK, Al-Mosaui, AM, Al-Rubaea, AH (2013). Prevalence of parasitic infection and relationship with anemia in Al-Doullab village, Babylon province, Iraq. Egy J Exp Biol (Zool), 1(7), 300-309.
Ak M, Keleş E, Karacasu F, Pektaş B, Akkafa F, Özgür S, Şahinöz S, Özçırpıcı B, Bozkurt Aİ, Şahinöz T (2006) The distribution of the intestinal parasitic diseases in the Southeast Anatolian (GAP= SEAP) region of Turkey. Parasitol Res 99:146-152
Doni NY, Gurses G, Simsek Z, Zeyrek FY (2015) Prevalence and associated risk factors of intestial parasites among children of farm workers in the southeastern Anatolian region of Turkey. Annal Agricult Environ Med 22 (3)
Mehraj V, Hatcher J, Akhtar S, Rafique G, Beg MA (2008) Prevalence and factors associated with intestinal parasitic infection among children in an urban slum of Karachi. PloS One 3 (11):e3680
Korzeniewski K, Augustynowicz A, Smoleń A, Lass A (2015) Epidemiology of intestinal parasitic infections in school children in Ghazni province, eastern Afghanistan. Pakistan J Med Sci 31 (6):1421
Al-Mekhlafi AM, Abdul-Ghani R, Al-Eryani SM, Saif-Ali R, Mahdy MA (2016) School-based prevalence of intestinal parasitic infections and associated risk factors in rural communities of Sana'a, Yemen. Act Trop 163:135-141
Amer OH, Ashankyty IM, Haouas NAS (2016) Prevalence of intestinal parasite infections among patients in local public hospitals of Hail, Northwestern Saudi Arabia. Asian Pac J Trop Med 9 (1):44-48
Dhanabal J, Selvadoss PP, Muthuswamy K (2014) Comparative study of the prevalence of intestinal parasites in low socioeconomic areas from South Chennai, India. J Parasitol Res 2014
Matthys B, Bobieva M, Karimova G, Mengliboeva Z, Jean-Richard V, Hoimnazarova M, Kurbonova M, Lohourignon LK, Utzinger J, Wyss K (2011) Prevalence and risk factors of helminths and intestinal protozoa infections among children from primary schools in western Tajikistan. Parasite Vectors 4:1-13
Seguí R, Muñoz-Antoli C, Klisiowicz DR, Oishi CY, Köster PC, de Lucio A, Hernández-de-Mingo M, Puente P, Toledo R, Esteban JG (2018) Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey. Parasite Vectors 11 (1):1-19
Molina N, Pezzani B, Ciarmela M, Orden A, Rosa D, Apezteguía M, Basualdo J, Minvielle M (2011) Intestinal parasites and genotypes of Giardia intestinalis in school children from Berisso, Argentina. J Infect Develop Countr 5 (07):527-534
Choi B, Kim B (2017) Prevalence and risk factors of intestinal parasite infection among schoolchildren in the peripheral highland regions of Huanuco, Peru. Osong Public Health Res Perspectives 8 (5):302
Derso A, Nibret E, Munshea A (2016) Prevalence of intestinal parasitic infections and associated risk factors among pregnant women attending antenatal care center at Felege Hiwot Referral Hospital, northwest Ethiopia. BMC Infect Dis 16 (1):1-7
Mbae CK, Nokes DJ, Mulinge E, Nyambura J, Waruru A, Kariuki S (2013) Intestinal parasitic infections in children presenting with diarrhoea in outpatient and inpatient settings in an informal settlement of Nairobi, Kenya. BMC Infect Dis 13 (1):1-11
Babiker M, Ali bS, Ahmed E (2009) Frequency of intestinal parasites among food-handlers in Khartoum, Sudan. EMHJ-East Mediterranean Health J, 15 (5), 1098-1104, 2009
Nxasana N, Baba K, Bhat V, Vasaikar S (2013) Prevalence of intestinal parasites in primary school children of Mthatha, Eastern Cape province, South Africa. Annals Med Health Sci Res 3 (4):511-516
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