Investigation of GNB1 derivative circular RNAs hsa_circ_0009361 and hsa_circ_0009362 expressions in colorectal cancer patients: potential new diagnostic factors
Gastroenterology and Hepatology from Bed to Bench,
Vol. 17 No. 1 (2024),
7 January 2024
https://doi.org/10.22037/ghfbb.v17i1.2863
Abstract
Aim: We aim to investigate the relation of these two circular RNAs with clinicopathological data of colorectal cancer patients.
Background: Circular RNAs (circRNAs) are implicated in the progression and development of Colorectal cancer (CRC). CircRNAs have been recognized as diagnostic and prognostic biomarkers and opening up a new window in order to comprehend the molecular basis of CRC. Given the significance of circRNAs and the G protein subunit b1 (GNB1) gene in malignancies, the goal of the current investigation was to determine the expression levels of GNB1 derivative circular RNAs circGNB1 (hsa_circ_0009361 and hsa_circ_0009362) in CRC and nearby control tissues.
Methods: The expression levels of the GNB1 derivative circular RNAs hsa_circ_0009361 and hsa_circ_0009362 in 45 CRC tissues and nearby control tissues were evaluated using the quantitative real-time PCR (qRT-PCR) method. All other data analyzed by GraphPad Prism software (La Jolla, CA).
Furthermore, we analyzed the diagnostic power of the mentioned circRNAs by plotting the receiver operating characteristic (ROC) curve. The association between the expression levels of hsa_circ_0009361 and hsa_circ_0009362 was evaluated using correlation analysis.
Result: Our results revealed that the expression levels of hsa_circ_0009361 and hsa_circ_0009362 were significantly down-regulated in CRC tissues compared to the adjacent control group. Analysis of patients’clinicopathological features shows that, expressions hsa_circ_0009361 and hsa_circ_0009362 were differently related with lymph vascular invasion (P < 0.001). ROC curve results have shown that these circRNAs are good candidate diagnostic biomarkers in CRCs. Pearson’s correlation test showed a positive correlation between hsa_circ_0009361 and hsa_circ_0009362 expression levels (P < 0.0001).
Conclusion: These results revealed that hsa_circ_0009361 and hsa_circ_0009362 expression levels may be used as possible diagnostic biomarkers for CRC.
- Cancer
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References
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