Hypolipidemic and hepatoprotective effects of corn silk extract in nicotine-administered male mice
Gastroenterology and Hepatology from Bed to Bench,
Vol. 17 No. 1 (2024),
7 January 2024
https://doi.org/10.22037/ghfbb.v17i1.2806
Abstract
Aim: This study is done to investigate the hypolipidemic and hepatoprotective effects of corn silk extract in nicotine-administered male mice.
Background: Nicotine can induce pathophysiological effects in the liver tissue through oxidative stress and damage cells. Corn silk can improve liver function with its antioxidant effects.
Methods: In this experimental study, 30 male NMRI mice (25-30 gr) were divided into 5 groups: controls, sham, nicotine 2.5 mg/kg, nicotine+aqueous extract of corn silk 400 mg/kg, and nicotine+methanolic extract of corn silk 400 mg/kg for 1 month. One day after the last nicotine and extracts consumption, the serum samples were performed for biochemical measurement, and the supernatant of the homogenized liver was administered for antioxidant variables assessment.
Results: There was no significant difference in the body weight of different groups. Liver weight and GSH decreased in the nicotine group compared to the control group (P<0.05). Triglycerides, total cholesterol, HDL-C, LDL-C, liver enzymes, and MDA increased in the nicotine group compared to the control group (P<0.05). Also, the expansion of sinusoids, the presence of inflammatory cells, and necrosis of liver cells were observed in the nicotine group compared to the control group. Using aqueous and methanolic extracts of corn silk in mice receiving nicotine led to the improvement of the mentioned variables (P<0.05).
Conclusion: The results of this study showed that the use of nicotine can lead to the induction of hepatotoxicity. The use of aqueous and methanolic extracts of corn silk improved them through its antioxidant activity.
- Nicotine, Corn silk, liver, Mouse.
How to Cite
References
Fagerström K. Nicotine: pharmacology, toxicity and therapeutic use. J Smok Cessat 2014;9:53-59.
Ni G, Zhang X, Afedo SY, Rui R. Evaluation of the protective effects of icariin on nicotine-induced reproductive toxicity in male mouse -a pilot study. Reprod Biol Endocrinol 2020;18:65.
Salahipour MH, Hasanzadeh S, Malekinejad H. Ameliorative effects of Achillea millefolium inflorescences alcoholic extract against nicotine-induced reproductive failure in rat. Exp Toxicol Pathol 2017;69:504-516.
Salahshoor M, Mohamadian S, Kakabaraei S, Roshankhah S, Jalili C. Curcumin improves liver damage in male mice exposed to nicotine. J Tradit Complement Med 2016;6:176-183.
Abushofa FA, Azab AE, Alkadrawy S. Hepatic pathophysiological changes induced by nicotine and/or sodium nitrite injection in male albino rats. East African Scholars J Med Sci 2019;2:184-196.
Jia Y, Gao X, Xue Z, Wang Y, Lu Y, Zhang M, et al. Characterization, antioxidant activities, and inhibition on α-glucosidase activity of corn silk polysaccharides obtained by different extraction methods. Int J Biol Macromol 2020;163:1640-1648.
Wans EM, Ahmed MM, Mousa AA, Tahoun EA, Orabi SH. Ameliorative effects of corn silk extract on acetaminophen-induced renal toxicity in rats. Environ Sci Pollut Res Int 2021;28:1762-1774.
Hasanudin K, Hashim P, Mustafa S. Corn silk (Stigma maydis) in healthcare: a phytochemical and pharmacological review. Molecules 2012;17:9697-9715.
Nessa N, Ifmaily I, Putri Y, Asra RJAJoPR, Development. Hepatoprotector effect of corn silk ethanol extract (stigma maydis) on paracetamol-induced white male rats. Asian J Pharm Res Dev 2021;9:10-16.
Saheed S, Oladipipo AE, Abdulazeez AA, Olarewaju SA, Ismaila NO, Emmanuel IA, et al. Toxicological evaluations of Stigma maydis (corn silk) aqueous extract on hematological and lipid parameters in Wistar rats. Toxicol Rep 2015;2:638-644.
Salahshoor MR, Khazaei M, Jalili C, Keivan M. Crocin improves damage induced by nicotine on a number of reproductive parameters in male mice. Int J Fertil Steril 2016;10:71-78.
Ernst C, Eling N, Martinez-Jimenez CP, Marioni JC, Odom DT. Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis. Nat Commun 2019;10:1251.
Ahangarpour A, Oroojan AA, Badavi M. Exendin-4 protects mice from D-galactose-induced hepatic and pancreatic dysfunction. Pathol Aging Age Relat Dis 2018;8:1418593.
Ahangarpour A, Shabani R, Farbood Y. The effect of betulinic acid on leptin, adiponectin, hepatic enzyme levels and lipid profiles in streptozotocin–nicotinamide-induced diabetic mice. Res Pharm Sci 2018;13:142-148.
Oroojan AA, Ahangarpour A, Paknejad B, Zareian P, Hami Z, Abtahi SR. Effects of myricitrin and solid lipid nanoparticle-containing myricitrin on reproductive system disorders induced by diabetes in male mouse. World J Mens Health 2021;39:147.
Khademi F, Totonchi H, Mohammadi N, Zare R, Zal F. Nicotine-induced oxidative stress in human primary endometrial cells. Int J Toxicol 2019;38:202-208.
Chattopadhyay K, Chattopadhyay BD. Effect of nicotine on lipid profile, peroxidation & antioxidant enzymes in female rats with restricted dietary protein. Indian J Med Res 2008;127:571-576.
Chattopadhyay K, Mondal S, Chattopadhyay B, Ghosh S. Ameliorative effect of sesame lignans on nicotine toxicity in rats. Food Chem Toxicol 2010;48:3215-3220.
Yarahmadi A, Zal F, Bolouki A. Protective effects of quercetin on nicotine induced oxidative stress in ‘HepG2 cells’. Toxicol Mech Methods 2017;27:609-614.
Husain K, Scott BR, Reddy SK, Somani SM. Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system. Alcohol 2001;25:89-97.
El-Sherbeeny NA, Nader MA, Attia GM, Ateyya H. Agmatine protects rat liver from nicotine-induced hepatic damage via antioxidative, antiapoptotic, and antifibrotic pathways. Naunyn-Schmiedeberg's Arch Pharmacol 2016;389:1341-1351.
Jalili C, Tabatabaei H, Kakaberiei S, Roshankhah S, Salahshoor MR. Protective role of Crocin against nicotine-induced damages on male mice liver. Int J Prev Med 2015;6:92.
Xia X, Ma Y, Xing X, Huang C, Li L, Gui G, et al. Antioxidant and hepatoprotective effect of different extracts of guizhencao (herba bidentis bipinnatae) against liver injury in hyperlipidemia rats. J Tradit Chin Med 2013;33:518-523.
Dey S, Roy S. Role of reduced glutathione in the amelioration of nicotine-induced oxidative stress. Bull Environ Contam Toxicol 2010;84:385-389.
Shi S, Yu B, Li W, Shan J, Ma T. Corn silk decoction for blood lipid in patients with angina pectoris: a systematic review and meta‐analysis. Phytother Res 2019;33:2862-2869.
Chu ZS, Yu ZL, Pan SY, Jia ZH, Wang XY, Zhang Y, et al. A comparative study between Wuweizi seed and its post-ethanol extraction residue in normal and hypercholesterolemic mice. Lipids Health Dis 2015;14:93.
Helen A, Krishnakumar K, Vijayammal P, Augusti K. Antioxidant effect of onion oil (Allium cepa. Linn) on the damages induced by nicotine in rats as compared to alpha-tocopherol. Toxicol Lett 2000;116:61-68.
- Abstract Viewed: 162 times
- PDF Downloaded: 117 times