High Viral Load Detection of Human Cosavirus in Iranian Pediatric Patients with Acute Gastroenteritis
Gastroenterology and Hepatology from Bed to Bench,
30 December 2021
Aim: The present study was implemented an RT-qPCR assay for detection and quantification of human Cosavirus in stool specimens among pediatric patients involved in acute gastroenteritis.
Background: Human Cosavirus is a newly recognized virus that seems to be partly related to acute gastroenteritis in pediatric patients. However, the relationship between human Cosavirus and diseases in humans is unclear.
Methods: In this study, a total of 160 stool samples from January 2018 to December 2019 were collected from presenting pediatric patients with acute gastroenteritis in a Karaj hospital, Iran. After viral RNA extraction, the RT-qPCR was performed to amplify the 5’UTR region of the human Cosavirus genome and viral load was analyzed.
Results: Out of the 160 samples tested, the human Cosavirus genomic RNA was detected in 4/160 (2.5%) of stool samples. The maximum viral load was determined to be 4.6×106 copies/ml in one sample obtained from 4 years’ patient.
As a new member of Picornaviridae family, for the first time occurrence of human Cosavirus illustrated in fecal samples of pediatric patients with acute gastroenteritis in Iran.
- Cosavirus, Acute gastroenteritis, pediatric patients, stool
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