Significant changes of CD4, FOXP3, CD25 and IL 6 mRNA expression levels in Covid 19 patients
Gastroenterology and Hepatology from Bed to Bench,
20 September 2020
Aim: evaluation the expression level of CD4 (+) FoxP3(+) CD25(+) T cells in COVID-19 patients and assessed its correlation with the level of IL6.
Background: there is insufficient information regarding Tregs mRNA expression level and also the regulation of IL6 cascade in COVID-19 patients.
Method: Whole blood samples of 30 COVID-19 patients and also 12 healthy people were collected during 2020. Total RNA was extracted and after adjusting the RNA concentrations, cDNA synthesis was performed using cDNA synthesis kit. To evaluate the expression levels of targeted genes, q real-time PCR was performed.
Results: Based on the results, the mRNA expression levels of TCD4 (5-fold changes), CD25 and Foxp3 (2- and 3-fold, respectively) in COVID-19 patients significantly downregulated in comparison to the healthy controls (P-value<0.0001). Besides, in the line of our results, the expression levels of IL6 represented almost 20-fold increase in COVID-19 patients compared to healthy controls
Conclusion: These data proposed that Treg cells may be a biomarker of severity and remark the probability that antibodies directing IL-6 might moderating lymphocyte stability
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