Effects of butyric acid and arsenic on isolated liver mitochondria and pancreatic islets of male mouse
Gastroenterology and Hepatology from Bed to Bench,
Vol. 10 No. 1 (2017),
11 February 2017
Aim: The aim of present study was to evaluate the different doses of Butyric acid (BA) and Arsenic (As) on mouse liver mitochondria oxidative stress and pancreatic islets insulin secretion.
Background: BA is found in many foods and As is a toxic metal in drinking water. They can induce oxidative stress in some tissue.
Materials and Methods: In this experimental study, Liver mitochondria were isolated by administration of different centrifugation method and pancreatic islets of mice were isolated by a collagenase method. Mitochondria by BA (35, 75, 150, 300 ?M) and As (20, 50, 100, 200 ?M) and the islets were incubated by BA (250, 500, 1000, 1500 ?M) and As (0, 50, 100, 200 ?M) for 1 hour. At the end of experiment mitochondrial viability and membrane potential, ROS, MDA, GSH and islets insulin secretion were measured by their specific methods.
Results: BA and As administration increased mitochondrial levels of ROS, MDA and decreased GSH and pancreatic islet insulin secretion in a dose dependent manner (p < 0.05). The exact mitochondria toxic concentrations were 75 ?M for BA and 100 ?M for As. Also, the doses of 1000 ?M for BA and 100 ?M for As were consider as reducing concentrations for islets insulin secretion. Additionally, coadministration of these doses of BA and As revealed an additive effect on their own tissues variables.
Conclusion: Alone or in combination administration of BA and As induced oxidative stress in liver mitochondria and decreased insulin secretion of pancreatic islets.
- Butyric acid
- Liver mitochondria
How to Cite
Smith JG, Yokoyama WH, German JB. Butyric acid from the diet: actions at the level of gene expression. Crit Rev Food Sci Nutr 1998; 38(4): 259-97.
Dabbagh MN, Fürll M, Schäfer M. [Sub acute butyric acid burden in cattle. 1. Clinical results and effects on the carbohydrate-fat metabolism and the liver function of young fattening bulls]. Arch Exp Veterinar Med 1989; 43(3): 427-35. [In German]
Cueno ME, Imai K, Tamura M, Ochiai K. Butyric acid-induced rat jugular blood cytosolic oxidative stress is associated with SIRT1decrease. Cell Stress Chaperones 2014; 19(2): 295-8.
Cueno ME, Kamio N, Seki K, Kurita-Ochiai T, Ochiai K. High butyric acid amounts induce oxidative stress, alter calcium homeostasis, and cause neurite retraction in nerve growth factor-treated PC12 cells. Cell Stress Chaperones 2015; 20(4): 709-13
Itoh Y, Kawamata Y, Harada M, Kobayashi M, Fujii R, Fukusumi S, et al. Free fatty acids regulate insulin secretion from pancreatic beta cells through GPR40. Nature 2003; 422(6928): 173-6.
Schönfeld P, Wojtczak L. Fatty acids decrease mitochondrial generation of reactive oxygen species atthe reverse electron transport but increase it at the forward transport. Biochimica et Biophysica Acta 2007; 1767 (8): 1032–40.
Lesnefsky EJ, Moghaddas S, Tandler B, Kerner J, Hoppel CL. Mitochondrial dysfunction in cardiac disease: ischemia–reperfusion, aging, and heart failure. J Mol Cell Cardiol 2001; 33 (6): 1065–89.
Mathews V, Binu P, Sauganth Paul M, Abhilash M, Manju A, Harikumaran Nair R. Hepatoprotective efficacy of curcumin against arsenic trioxide toxicity. Asian Pac J Trop Biomed 2012; 2(2): S706–S711.
Dwivedi N, Mehta A, Yadav A, Binukumar BK, Gill KD, Flora SJ. MiADMSA reverses impaired mitochondrial energy metabolism and neuronal apoptotic cell death after arsenic exposure in rats. Toxicol Appl Pharmacol 2011; 256(3): 241–8.
Ramanathan K, Shila S, Kumaran S, Panneerselvam C. Ascorbic acid and a-tocopherol as potent modulators on arsenic induced toxicity in mitochondria. J Nutrit Biochem 2003; 14(7): 416–20.
Niedzwiecki MM, Hall MN, Liu X, Slavkovich V, Ilievski V, Levy D, et al. Interaction of plasma glutathione redox and folate deficiency on arsenic methylation capacity in Bangladeshi adults. Free Rad Biol Med 2014; 73: 67–74.
Yen CC, Ho TJ, Wu CC, Chang CF, Su CC, Chen YW, et al. Inorganic arsenic causes cell apoptosis in mouse cerebrum through an oxidative stress-regulated signaling pathway. Arch Toxicol 2011; 85: 565-75.
Flora SJ. Arsenic-induced oxidative stress and its reversibility. Free Rad Biol Med 2011; 51(2): 257–81.
Ambrosio F, Brown E, Stolz D, Ferrari R, Goodpaster B, Deasy B, et al. Arsenic induces sustained impairment of skeletal muscle and muscle progenitor cell ultra structure and bioenergetics. Free Rad Biol Med 2014; 74: 64–73.
Liu J, Waalkes MP. Liver is a target of arsenic carcinogenesis. Toxicol Sci 2008; 105: 24-32.
Belyaeva EA, Korotkov SM, Saris NE. In vitro modulation of heavy metal-induced rat liver mitochondria dysfunction: a comparison of copper and mercury with cadmium. J Trace Elem Med Biol 2011; 25 (Suppl. 1): 63–73.
Hosseini MJ, Shaki F, Ghazi-Khansari M, Pourahmad J. Toxicity of arsenic (III) on isolated liver mitochondria: a new mechanistic approach. Iran J Pharm Res 2013; 12 (Suppl.1): 119–36
Ahangarpour A, Alboghobeish S, Rezaei M, Khodayar MJ, Oroojan AA, Zaidy Vand M. Evaluation of diabetogenic mechanism of high fat diet in combination with Arsenic exposure in male mice. Iran J Pharm Res 2016. [under review].
Bustamante J, Nutt L, Orrenius S, Gogvadze V. Arsenic stimulates release of cytochrome c from isolated mitochondria via induction of mitochondrial permeability transition. Toxicol Appl Pharmacol 2005; 207(2):110–6.
Keshtzar E, Khodayar MJ, Javadipour M, Ghaffari MA, Bolduc DL, Rezaei M. Ellagic acid protects against arsenic toxicity in isolated rat mitochondria possibly through the maintaining of complex II. Hum Exp Toxicol 2015; pii: 0960327115618247. [In press]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54.
Pourahmad J, Rabiei M, Jokar F, O›brien PJ. A comparison of hepatocyte cytotoxic mechanisms for chromate and arsenite. Toxicology 2005; 206: 449-60.
Furuno T, Kanno T, Arita K, Asami M, Utsumi T, Doi Y, et al. Roles of long chain fatty acids and carnitine in mitochondrial membrane permeability transition. Biochem Pharmacol 2001; 62: 1037–46.
Penzo D, Tagliapietra C, Colonna R, Petronilli V, Bernardi P. Effects of fatty acids on mitochondria: implications for cell death. Biochimica et Biophysica Acta 2002; 1555: 160–5.
Korge P, Honda HM, Weiss JN. Effects of fatty acids in isolated mitochondria: implications for ischemic injury and cardio protection. Am J Physiol Heart Circ Physiol 2003; 285: H259–H269.
Sutton R, Peters M, McShane P, Gray DWR, Morris PJ. An improved method for the isolation of islets of Langerhans from the adult rat pancreas. Transplant Proc 1986; 18: 1819–20.
Amaral AG, Rafacho A, Machado de Oliveira CA, Batista TM, Ribeiro RA, Latorraca MQ, et al. Leucine supplementation augments insulin secretion in pancreatic islets of malnourished mice. Pancreas 2010; 39(6): 847-55.
O’Dowd JF. The isolation and purification of rodent pancreatic islets of Langerhans. Methods Mol Biol 2009; 560: 37–42.
Rezaei M, Salimi A, Taghidust M, Naserzadeh P, Goudarzi G, Seydi E, et al. A comparison of toxicity mechanisms of dust storm particles collectedin the southwest of Iran on lung and skin using isolated mitochondria. Toxicol Environ Chem 2014; 96(5): 814–30.
Shaki F, Hosseini MJ, Ghazi-Khansari M, Pourahmad J. Toxicity of depleted uranium on isolated rat kidney mitochondria. Biochimica et Biophysica Acta 2012; 1820: 1940–50.
Naserzadeha P, Hosseini MJ, Arbabia S, Pourahmad J. A Comparison of Toxicity Mechanisms of Cigarette Smoke on Isolated. Mitochondria Obtained from Rat Liver and Skin. Iran J Pharm Res 2015; 14 (1): 271-7.
Baracca A, Sgarbi G, Solaini G, Lenaz G. Rhodamine 123 as a probe of mitochondrial membrane potential: evaluation of proton flux through F0 during ATP synthesis. Biochim Biophys Acta 2003; 1606: 137–46.
Hassani S, Yaghoubi H, Khosrokhavar R, Jafarian I, Mashayekhi V, Hosseini MJ, et al. Mechanistic view for toxic effects of arsenic on isolated rat kidney and brain mitochondria. Biologia 2015; 70(5): 683–9.
Sadegh C, Schreck RP. The spectroscopic determination of aqueous sulfite using Ellman's reagent. MIT Undergradu Res J 2003; 8: 39–43.
Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978; 90(1): 37-43.
Jijakli H, Zhang Y, Sener A, Malaisse WJ. Tritiated taurine handling by isolated rat pancreatic islets. Endocrine 2006; 29(2): 331-9.
Oliveira CA, Paiva ME, Mota CA, Ribeiro C, Leme JA, Luciano E, et al. Exercise at anaerobic threshold intensity and insulin secretion by isolated pancreatic islets of rats. Islets 2010; 2(4): 240-6.
Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. The Role of Oxidative Stress and Antioxidants in Liver Diseases. Int J Mol Sci 2015; 16(11): 26087-124.
Dutta M, Ghosh D, Ghosh AK, Bose G, Chattopadhyay A, Rudra S, et al. High fat diet aggravates arsenic induced oxidative stress in rat heart and liver. Food ChemToxicol 2014; 66: 262-77.
Zgorzynska E, Wierzbicka-Ferszt A, Dziedzic B, Witusik-Perkowska M, Zwolinska A, Janas A, Walczewska A. Docosahexaenoic acid attenuates oxidative stress and protects human gingival fibroblasts against cytotoxicity induced by hydrogen peroxide and butyric acid. Arch Oral Biol 2015; 60(1): 144-53.
Prakash C, Kumar V. Chronic Arsenic Exposure-Induced Oxidative Stress is Mediated by Decreased Mitochondrial Biogenesis in Rat Liver. Biol Trace Elem Res 2016. [In press].
Prentki M, Nolan CJ. Islet beta cell failure in type 2 diabetes. J Clin Invest 2006; 116(7): 1802-12.
Huang CF, Yang CY, Chan DC, Wang CC, Huang KH, Wu CC, et al. Arsenic Exposure and Glucose Intolerance/Insulin Resistance in Estrogen-Deficient Female Mice. Environ Health Perspect 2015; 123(11): 1138-44.
Fridlyand LE, Philipson LH. Does the glucose-dependent insulin secretion mechanism itself cause oxidative stress in pancreatic beta-cells? Diabetes 2004; 53(8): 1942-8.
- Abstract Viewed: 691 times
- PDF Downloaded: 198 times