Isolation, characterization and tolerance survey of bacterial strains to cadmium in soils receiving Hamadan industrial parks wastewater treatment plant effluent
Journal of Health in the Field,
,
27 January 2017
چکیده
Background and Aims: Biosorption is a relatively new clean up method used for the removal of heavy metals from the environment. Entry of heavy metals into the environment may result in change in population structure and further alteration of resistance mechanism(s) in exposed microorganisms. The first question inthis study sought to determine the threshold of bacterial resistance to cadmium (Cd). The second question was to identify indigenous bacterial species capable of removing the Cd from the soil and consequently to achieve a more efficient biological treatment for industrial effluents.
Materials and Methods: Natural topsoil samples were obtained from three industrial parks in Hamadan, Iran. The concentration of Cd in soil samples was measured and thence the bacterial cultures were prepared. Following the screening process, the minimum inhibitory concentration [MIC] and minimum bactericidal concentration [MBC] were determined in order to find the threshold of bacterial tolerance. Indeed, the
adsorption capacity of bacterial strains came down in favor of the identification of resistant bacteria.
Results: Respectively, 42, 4 and 4 bacterial strains were identified for cultures having average cadmium concentrations of 0.78, 500 and 750 mg/L. This experiment did also detect that Tcd2 and Tcd4 showed the highest MIC with 1250 mg Cd/L. Moreover, TCd2, belonging to the family of Enterobacteriaceae and Enterobacter genus, demonstrated the maximum cadmium removal efficiency (30.2%).
Conclusion: The test was successful as it was able to identify the indigenous cadmium tolerant bacteria with high adsorption capacity. This finding has important implications for developing special biological wastewater treatment such as a biofilter in the cadmium-releasing industries.
Key words: Cadmium, Hamadan, Industrial park, Resistant bacteria, Soil
مراجع
Asbchin AS, Andres Y, Malekzdeh F. Biosorption and Optimization of Condition Uptake of Cesium by Bacterium. Water&Wastwater. 2011; 22 (4): 50-56.[In Persian].
Velasquez L, Dussan J. Biosorption and bioaccumulation of heavy metals on dead and living biomass of Bacillus sphaericus. Journal of hazardous materials. 2009;167(1) :713-6.
Soltaninejad SH, Emtiazi G, Mokhtari ST. Uptake of heavy metals [lead and zinc] by bacterial extracellular polymers. Journal of Microbial Biotechnology, Islamic Azad University. 2010; 2(7):23-8 [In Persian].
Sobolev D, Begonia M. Effects of heavy metal contamination upon soil microbes: lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers. International journal of environmental research and public health. 2008; 5(5):450-6.
Raja CE, Anbazhagan K, Selvam GS. Isolation and characterization of a metal-resistant Pseudomonas aeruginosa strain. World Journal of Microbiology and Biotechnology. 2006; 22(6):577-85.
/66
Dayani M, Mohammadi J, Naderi M. Geostatistical Analysis of Pb, Zn and Cd concentration in soil of Sepahanshahr suburb [south of Esfahan]. Journal of water and soil. 2009; 23(4): 67-76.
Tahmourespour A KR. Adaptability of Bacterical Isolates to Heavy Metals in Industrial Wastwater. Water&Wastwater. 2007;18(61):53-9.[In Persian].
Jalali M, Khanboluki G. Redistribution of zinc, cadmium, and lead among soil fractions in a sandy calcareous soil due to application of poultry litter. Environmental Monitoring and Assessment. 2008;136(1):327-335.[In Persian].
Jacob H, Dane G, Clarbe Topp. Method of Soil Analysis .part 4 [physical method]. Soil Science Society of America,Inc. Madison,Wisconsin,USA; 2002.
Congeevaram S, Dhanarani S, Park J, Dexilin M, Thamaraiselvi K. Biosorption of chromium and nickel by heavy metal resistant fungal and bacterial isolates. Journal of hazardous materials. 2007;146(1):270-7.
Gabr RM, Hassan SHA, Shoreit AAM. Biosorption of lead and nickel by living and non-living cells of Pseudomonas aeruginosa ASU 6a. International Biodeterioration & Biodegradation. 2008;62(2):195-203.
Yu J, Lei J, Yu H, Cai X, Zou G. Chemical composition and antimicrobial activity of the essential oil of Scutellaria barbata. Phytochemistry. 2004;65 ( 7):881-4.
Lu WB, Shi JJ, Wang CH, Chang JS. Biosorption of lead, copper and cadmium by an indigenous isolate Enterobacter sp. J1 possessing high heavy-metal resistance. Journal of hazardous materials. 2006;134(1):80-86.
Hoodchi M, Tahmourespour A, Azadani N A. Determine the threshold of bacteria, lead, zinc, cadmium in industrial wastewater. Ecology 2010; 56(36): 75-86.[In Persian].
Lipson D, Schadt C, Schmidt S. Changes in soil microbial community structure and function in an alpine dry meadow following spring snow melt. Microbial Ecology 2002; 43(3): 307-14
Rousk J, Brookes PC, Baath E. Investigating the mechanisms for the opposing pH relationships of fungal and bacterial growth in soil. Soil Biology and Biochemistry 2010; 42(6): 926-34.
Pais I, Jones Jr JB. The handbook of trace elements: CRC Press; 1997.
Morin S, Duong TT, Herlory O, Feurtet-Mazel As, Coste M. Cadmium toxicity and bioaccumulation in freshwater biofilms. Archives of environmental contamination and toxicology. 2008; 54(2): 173-86.
Ansari MI, Malik A. Biosorption of nickel and cadmium by metal resistant bacterial isolates from agricultural soil irrigated with industrial wastewater. Bioresource technology. 2007; 98[16]: 3149-53.
Terry PA, Stone W. Biosorption of cadmium and copper contaminated water by Scenedesmus abundans. Chemosphere. 2002; 47(3): 249-55.
Kozdroj J, van Elsas JD. Response of the bacterial community to root exudates in soil polluted with heavy metals assessed by molecular and cultural approaches. Soil Biology and Biochemistry. 2000; 32(10):1405-17.
Jawetz E. Enterobacteriaceae In: Brooks GF, Butel JS, Morse SA eds. Medical Microbiology. Stanford-Connecticut. Appleton and Lange; 2004.
Kafilzadeh s, Abvalahrar H, Karegar, qodsi. Effect of cadmium toxicity and determine the range of resistance / tolerance to bacterial species detected in the river water and sediment study Choir Ray Fars Province .Journal of Public Health and Institute of Health Research. 2010; 8(1): 69-80. .[In Persian].
Piotrowska-Seget Z, Cycoń M, Kozdroj J. Metal-tolerant bacteria occurring in heavily polluted soil and mine spoil. Applied Soil Ecology 2005; 28(3): 237-46.
El-Deeb B. Plasmid Mediated tolerance and Removal of Heavy Metals by Enterobacter sp. American Journal of Biochemistry and Biotechnology 2009; 5[1]: 47.
Lu W-B, Shi J-J, Wang C-H, Chang J-S. Biosorption of lead, copper and cadmium by an indigenous isolate Enterobacter sp. J1 possessing high heavy-metal resistance. Journal of Hazardous Materials 2006; 134[1]: 80-6.
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