The Association between Interleukin-10 Gene Polymorphisms and Hepatitis B Virus: Evidence in Iran
Archives of Medical Laboratory Sciences,
Vol. 7 (2021),
13 March 2021
,
Page 1-7 (e7)
https://doi.org/10.22037/amls.v7.33557
Abstract
Background and Aim: Hepatitis B virus (HBV) is a human carcinogenesis agent. Interleukin 10 (IL-10) is a key anti-inflammatory cytokine, and single nucleotide polymorphisms in IL-10 gene promoter are correlated with infections caused by HBV. This research intended to assess the prevalence and genotype of HBV as well as the association between the polymorphisms of -819 and -1082 in the IL-10 gene with HBV in individuals with HBV infection in Qom Province, Iran
Methods: In this cross-sectional research, 360 individuals with chronic HBV infection and control group were involved between July 2018 and March 2019. HBV diagnosis was evaluated using ELISA and nested PCR assays. To determine polymorphisms in the IL-10 gene promoter in HBV positive and control samples, an allele-specific polymerase chain reaction technique was employed.
Results: The constructed phylogenetic trees for the HBsAg gene revealed that all sequences under study belong to genotype D and also, the majority of HBV samples presented similar sequences to the Iranian samples. Genotype frequencies of TT, TC and CC (polymorphism -819) were 82.2%, 11.6% and 6.1% for patients and 85%, 10.5% and 4.4% in control groups, respectively. Also, frequency of genotypes of AA, AG and GG (polymorphism -1082) were 45%, 43.8% and 11.1% for patients and 42.2%, 46.1% and 11.6% in control groups, respectively.
Conclusion: Here, we found no association among IL-10 gene polymorphisms in control and HBV-infected groups. However, more studies about the frequency of chronic HBV infection are necessary to be conducted.
*Corresponding Author: Mohsen Zargar; Email: zargar@qom-iau.ac.ir
Interleukins-10 Gene Polymorphism and Hepatitis B Virus: Evidence in Iran. Arch Med Lab Sci. 2021;7:1-7 (e7). https://doi.org/10.22037/amls.v7.33557
- Hepatitis B Virus
- Nested PCR
- Polymorphism
- Interleukin-10
- IL-10
How to Cite
References
Khalifa A, El-Saeed G, Jaiash D, Shady A, Ghonaim M, Eed E, et al. Interleukins-10 And 18 Genes Polymorphisms In Hepatitis B Virus Infected Saudi Patients. Research Journal of Immunology. 2016;9(1):1-8.
Popovici O, Ursu RG, Azoicai D, Iancu LS. HBV genotypes circulation in pregnant women in Romania: a pilot study. Revista Romana de Medicina de Laborator. 2020;28(1):91-8.
Riaz MN, Faheem M, Anwar MA, Raheel U, Badshah Y, Akhtar H, et al. PCR-based molecular diagnosis of hepatitis virus (HBV and HDV) in HCV infected patients and their biochemical study. Journal of pathogens. 2016;2016:1-8.
Glebe D, Bremer CM, editors. The molecular virology of hepatitis B virus. Seminars in liver disease; Thieme Medical Publishers.2013;33(2):103-112.
Sagnelli E, Alessio L, Sagnelli C, Gualdieri L, Pisaturo M, Minichini C, et al. Hepatitis B virus genotypes, epidemiological characteristics, and clinical presentation of HBV chronic infection in immigrant populations living in southern Italy. Hepatitis monthly. 2017;17(8): e13260.
Das UN. Beneficial role of bioactive lipids in the pathobiology, prevention, and management of HBV, HCV and alcoholic hepatitis, NAFLD, and liver cirrhosis: A review. Journal of Advanced Research. 2019;17:17-29.
Chevaliez S, Pawlotsky J-M. New virological tools for screening, diagnosis and monitoring of hepatitis B and C in resource-limited settings. Journal of hepatology. 2018;69(4):916-26.
Bottecchia M, Souto FJ, Kycia M, Amendola M, Brandão CE, Niel C, et al. Hepatitis B virus genotypes and resistance mutations in patients under long term lamivudine therapy: characterization of genotype G in Brazil. BMC microbiology. 2008;8(1):1-11.
Azad AR, Zargar M, Zolfaghari MR, Mohammadbeigi A. The Prevalence of Hepatitis B and D Viruses and Evaluating YMDD Mutation in HBV-Suspected Patients in Qom Province, Iran. Jundishapur Journal of Microbiology. 2020;13(2):1-7.
Cheng S-T, Yuan D, Liu Y, Huang Y, Chen X, Yu H-B, et al. Interleukin-35 Level Is Reduced in Patients with Chronic Hepatitis B Virus Infection. International journal of medical sciences. 2018;15(2):188-194.
Perrillo R. Benefits and risks of interferon therapy for hepatitis B. Hepatology. 2009;49(S5):103-111.
Gao Q-J, Liu D-W, Zhang S-Y, Jia M, Wang L-M, Wu L-H, et al. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World Journal of Gastroenterology.2009;15(44):5610-5619.
Yang G, Liu J, Han S, Xie H, Du R, Yan Y, et al. Association between hepatitis B virus infection and HLA‐DRB1 genotyping in Shaanxi Han patients in northwestern China. Tissue antigens. 2007;69(2):170-5.
Wu J-F, Ni Y-H, Lin Y-T, Lee T-J, Hsu SH-J, Chen H-L, et al. Human interleukin-10 genotypes are associated with different precore/core gene mutation patterns in children with chronic hepatitis B virus infection. The Journal of pediatrics. 2011;158(5):808-13.
Truelove AL, Oleksyk TK, Shrestha S, Thio CL, Goedert JJ, Donfield SM, et al. Evaluation of IL10, IL19 and IL20 gene polymorphisms and chronic hepatitis B infection outcome. International journal of immunogenetics. 2008;35(3):255-64.
Shu C, Wang J, He Y, Song T, Chen Z, Tang S, et al. Effects of interleukin 10 polymorphisms on the development of hepatitis B virus infection: a systemic review and meta-analysis. International journal of clinical and experimental medicine. 2015;8(8):120-28.
Zamor PJ, Delemos AS, Russo MW. Viral hepatitis and hepatocellular carcinoma: etiology and management. Journal of gastrointestinal oncology. 2017;8(2):229-242.
McMahon BJ. The natural history of chronic hepatitis B virus infection. Hepatology. 2009;49(S5):45-55.
Ali SA, Donahue RM, Qureshi H, Vermund SH. Hepatitis B and hepatitis C in Pakistan: prevalence and risk factors. International journal of infectious diseases. 2009;13(1):9-19.
Rahimyan K, Vaezjalali M, Rostami M, Shokatpour N, Ahmadpour P. Prevalence of YMDD mutations in patients with chronic hepatitis B and renal transplant recipient patients after prolonged using of lamivudine. Iranian Journal of Medical Microbiology. 2016;9(4):73-8.
Bahri F, Kheirabad AK, Ghasemzadeh I, Shoja S, Gouklani H. Hepatitis Viruses B and D and Human Immunodeficiency Virus infections in hemodialysis patients in the south of Iran: prevalence and genotypes. Hepatitis monthly. 2016;16(1): e32971.
Behzadi MA, Leyva-Grado VH, Namayandeh M, Ziyaeyan A, Feyznezhad R, Dorzaban H, et al. Seroprevalence of viral hepatitis A, B, C, D and E viruses in the Hormozgan province southern Iran. BMC infectious diseases. 2019;19(1):1027-1039.
Mostaghni AA, Soltanian A, Mokhtari E, Japoni S, Mehrabani D. Seroprevalence of hepatitis B virus among hemodialysis patients in Bushehr province, southern Iran: HBV seroprevalence in hemodialysis patients. Hepatitis monthly. 2011;11(3):200-2.
Rybicka M, Woziwodzka A, Sznarkowska A, Romanowski T, Stalke P, Dręczewski M, et al. Genetic variation in IL-10 influences the progression of hepatitis B infection. International journal of infectious diseases. 2020;96:260-5.
Tseng LH, Lin MT, Shau WY, Lin WC, Chang FY, Chien KL, et al. Correlation of interleukin‐10 gene haplotype with hepatocellular carcinoma in Taiwan. Tissue antigens. 2006;67(2):127-33.
Zhang T-C, Pan F-M, Zhang L-Z, Gao Y-F, Zhang Z-H, Gao J, et al. A meta-analysis of the relation of polymorphism at sites −1082 and −592 of the IL-10 gene promoter with susceptibility and clearance to persistent hepatitis B virus infection in the Chinese population. Infection. 2011;39(1):21-7.
Sofian M, Kalantar E, Aghakhani A, Hosseini S, Banifazl M, Eslamifar A. No correlation between interleukin-10 gene promoter polymorphisms and hepatitis B virus infection outcome. Hepatitis monthly. 2013;13(5):e8803.
Bineshian F, Irajian G, Bagheri Mansoori MH, sharifi z. The relationship between IL-10 (-819 C/T) polymorphism and Hepatitis B infection. Iranian Journal of Medical Microbiology. 2016;10(3):47-53.
Mirfakhar FS, Mohebbi SR, Hosseini SM, Azimzadeh P, Derakhshani S, Sharifian A, et al. Evaluation of Interleukin 10 Gene Polymorphism (rs1800872A/C) Association with Chronic Hepatitis B Infection. Razi Journal of Medical Sciences. 2016;23(144):91-9.
Gao L, Chen X, Zhang L, Wu D, Zhao H, Niu J. Association of IL-10 polymorphisms with hepatitis B virus infection and outcome in Han population. European Journal of Medical Research. 2016;21(1):1-6.
Moudi B, Heidari Z, Mahmoudzadeh-Sagheb H, Hashemi M, Metanat M, Khosravi S, et al. Association between IL-10 gene promoter polymorphisms (-592 A/C,-819 T/C,-1082 A/G) and susceptibility to HBV infection in an Iranian population. Hepatitis monthly. 2016;16(2):e32427.
Shin HD, Park BL, Kim LH, Jung JH, Kim JY, Yoon JH, et al. Interleukin 10 haplotype associated with increased risk of hepatocellular carcinoma. Human molecular genetics. 2003;12(8):901-6.
Khalilian M, Hosseini SM, Madadgar O. Bovine leukemia virus detected in the breast tissue and blood of Iranian women. Microbial Pathogenesis. 2019;135:103566.
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