Berberine Improves Meat Quality and Carcass Traits in Broilers Challenged with Mycotoxins
Archives of Medical Laboratory Sciences,
Vol. 6 (2020),
20 January 2020
,
Page 1-9 (e23)
https://doi.org/10.22037/amls.v6.33330
Abstract
Background and Aim: Mycotoxin-contaminated feed causes significant concern in poultry production and public health because of serious economic losses and health problems caused by them. Berberine hydrochloride (Berberine), a natural plant alkaloid derived from Chinese medicine, is characterized by diverse pharmacological effects. This study is designed to evaluate the effects of different levels of Berberine (BBR) on carcass traits and meat quality of broilers fed diets contaminated with Aflatoxin B1 (AFB) and Ochratoxin A (OCT).
Methods: A 42-day floor pen trial was performed with 288 Ross 308 broilers. A randomized design, with 4 replicates of 8 birds each, was conducted with the following 9 treatments: (1) negative control diet with no additives (NC); (2) NC + 2 ppm AFB (positive control AFB; PCAFB); (3) NC + 2 ppm OCT (positive control OCT; PCOCT); (4) PCAFB + 200 mg/kg BBR; (5) PCAFB + 400 mg/kg BBR; (6) PCAFB + 600 mg/kg BBR; (7) PCOCT + 200 mg/kg BBR; (8) PCOCT + 400 mg/kg BBR; and (9) PCOCT + 600 mg/kg BBR. At the end of the experiment, from every group, eight birds were selected, slaughtered, and subjected to analyses. The analyzed parameters carcass and cut yields and meat quality according to water-holding capacity (WHC) and breast level MDA.
Results: Both PCAFB and PCOTA diets decreased carcass relative weight, breast level MDA, and WHC values compared with the NC diet (p<0.05). Meat level of MDA in PCAFB and PCOTA treatments were higher than in the NC treatment (p˂0.05), and supplementation with BBR at multiple levels partially these effects (p˂0.05). The addition of 600 mg/kg BBR to PCAFB diets increased WHC value to levels not different from that in the NC group (p>0.05). Carcass efficiency in PCAFB and PCOTA treatments was lower than in the NC treatment (p˂0.05), and supplementation with BBR at multiple levels partially or fully reversed these effects. The addition of 600 mg/kg BBR to PCAFB diet increased carcass relative weight compared to PCAFB alone (p>0.05).
Conclusion: These data provided supplementation of BBR (600 mg/kg) improves meat quality in broiler fed diet contaminated with mycotoxins.
*Corresponding Author: Nazar Afzali; Email: nafzali@birjand.ac.ir
Please cite this article as: Malekinezhad P, Afzali N, Farhangfar SH, Omidi A, Mohammadi A. Berberine Improves Meat Quality and Carcass Traits in Broilers Challenged with Mycotoxins. Arch Med Lab Sci. 2020;6:1-9 (e23). https://doi.org/10.22037/amls.v6.33330
- Berberine, Carcass, Malondialdehyde, Mycotoxin, water holding capacity
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References
Shahidi, F. and Y. Zhong, Measurement of antioxidant activity. Journal of Functional Foods, 2015. 18: p. 757-781.
Gaya, L.d.G., et al., Estimates of heritability and genetic correlations for meat quality traits in broilers %J Scientia Agricola. 2011. 68: p. 620-625.
Le Bihan-Duval, E., et al., Chicken meat quality: genetic variability and relationship with growth and muscle characteristics. BMC Genetics, 2008. 9(1): p. 53.
Nissen, P.M. and J.F. Young, Creatine Monohydrate and Glucose Supplementation to Slow- and Fast-Growing Chickens Changes the Postmortem pH in Pectoralis Major. Poultry Science, 2006. 85(6): p. 1038-1044.
Luna, A., et al., Effects of thymol and carvacrol feed supplementation on lipid oxidation in broiler meat. Poultry Science, 2010. 89(2): p. 366-370.
Denli, M. and J.F.J.T. Perez, Ochratoxins in feed, a risk for animal and human health: control strategies. 2010. 2(5): p. 1065-1077.
Diaz, G.J., E. Calabrese, and R. Blain, Aflatoxicosis in Chickens (Gallus gallus): An Example of Hormesis? Poultry Science, 2008. 87(4): p. 727-732.
Sorrenti, V., et al., Toxicity of ochratoxin a and its modulation by antioxidants: a review. Toxins, 2013. 5(10): p. 1742-1766.
Salem, R., et al., Effect of probiotic supplement on aflatoxicosis and gene expression in the liver of broiler chicken. Environmental Toxicology and Pharmacology, 2018. 60: p. 118-127.
Yarru, L.P., et al., Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks. Poult Sci, 2009. 88(2): p. 360-71.
Iqbal, S.Z., et al., Natural incidence of aflatoxins, ochratoxin A and zearalenone in chicken meat and eggs. Food Control, 2014. 43: p. 98-103.
Wang, W.-J., et al., Effects of aflatoxin B1 on mitochondrial respiration, ROS generation and apoptosis in broiler cardiomyocytes. 2017. 88(10): p. 1561-1568.
Yenilmez, A., et al., Antioxidant effects of melatonin and coenzyme Q10 on oxidative damage caused by single-dose ochratoxin A in rat kidney. Chin J Physiol, 2010. 53(5): p. 310-7.
Erdélyi, M., et al., Changes in the regulation and activity of glutathione redox system, and lipid peroxidation processes in short-term aflatoxin B1 exposure in liver of laying hens. 2018. 102(4): p. 947-952.
Liu, Y. and W. Wang, Aflatoxin B1 impairs mitochondrial functions, activates ROS generation, induces apoptosis and involves Nrf2 signal pathway in primary broiler hepatocytes. 2016. 87(12): p. 1490-1500.
Maurya, B.K. and S.K. Trigun, Fisetin Modulates Antioxidant Enzymes and Inflammatory Factors to Inhibit Aflatoxin-B1 Induced Hepatocellular Carcinoma in Rats. Oxidative Medicine and Cellular Longevity, 2016. 2016: p. 1972793.
László, P., et al., Effect of combined treatment with aflatoxin B 1 and T-2 toxin and metabolites on some production traits and lipid peroxide status parameters of broiler chickens. Acta Veterinaria Hungarica, 2009. 57(1): p. 75-84.
Imanshahidi, M. and H. Hosseinzadeh, Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. 2008. 22(8): p. 999-1012.
Zuo, F., et al., Pharmacokinetics of berberine and its main metabolites in conventional and pseudo germ-free rats determined by liquid chromatography/ion trap mass spectrometry. Drug Metab Dispos, 2006. 34(12): p. 2064-72.
Küpeli, E., et al., A comparative study on the anti-inflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish Berberis species. Life Sciences, 2002. 72(6): p. 645-657.
Shen, S.S., W.H. Kinsey, and S.J. Lee, Protein tyrosine kinase-dependent release of intracellular calcium in the sea urchin egg. Dev Growth Differ, 1999. 41(3): p. 345-55.
Zhang, X., H. Ren, and L. Liu, (Effects of different dose berberine on hemodynamic parameters and (Ca2+) i of cardiac myocytes of diastolic heart failure rat model). Zhongguo Zhong yao za zhi= Zhongguo zhongyao zazhi= China journal of Chinese materia medica, 2008. 33(7): p. 818-821.
Feng, Y., et al., Hepatoprotective effects of berberine on carbon tetrachloride-induced acute hepatotoxicity in rats. 2010. 5(1): p. 33.
Kulkarni, S.K. and A. Dhir, On the mechanism of antidepressant-like action of berberine chloride. Eur J Pharmacol, 2008. 589(1-3): p. 163-72.
Shotwell, O.L., et al., Production of aflatoxin on rice. Applied Microbiology, 1966. 14(3): p. 425-428.
Trenk, H.L., M.E. Butz, and F.S. Chu, Production of ochratoxins in different cereal products by Aspergillus ochraceus. Applied microbiology, 1971. 21(6): p. 1032-1035.
Tangjitjaroenkun, J., R. Supabphol, and W.J.J.o.M.P.R. Chavasiri, Antioxidant effect of Zanthoxylum limonella Alston. 2012. 6(8): p. 1407-1414.
Wierbicki, E. and F.E. Deatherage, Water Content of Meats, Determination of Water-Holding Capacity of Fresh Meats. Journal of Agricultural and Food Chemistry, 1958. 6(5): p. 387-392.
Levine, R.L., et al., (49) Determination of carbonyl content in oxidatively modified proteins, in Methods in Enzymology. 1990, Academic Press. p. 464-478.
Institute, S., Base SAS 9.4 procedures guide. 2015: SAS Institute.
Bendary, E., et al., Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences, 2013. 58(2): p. 173-181.
Rajendran, J., et al., Simultaneous determination of berberine and β-sitosterol in the leaf extracts of Naravelia zeylanica by analytical methods and their in vitro Anti diabetic activity. Journal of Advanced Pharmacy Education & Research, 2014. 4(3).
Chen, W., et al., Pretreatment of rats with increased bioavailable berberine attenuates cerebral ischemia-reperfusion injury via down regulation of adenosine-5′ monophosphate kinase activity. 2016. 779: p. 80-90.
Naseem, M.N., et al., Hematological and Serum Biochemical Effects of Aflatoxin B1 Intoxication in Broilers Experimentally Infected with Fowl Adenovirus-4 (FAdV-4). 2018. 38(2).
Yunus, A.W., E. Razzazi-Fazeli, and J.J.T. Bohm, Aflatoxin B1 in affecting broiler’s performance, immunity, and gastrointestinal tract: A review of history and contemporary issues. 2011. 3(6): p. 566-590.
Wild, C.P. and P.C. Turner, The toxicology of aflatoxins as a basis for public health decisions. Mutagenesis, 2002. 17(6): p. 471-81.
Wangikar, P.B., et al., Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicology, 2005. 215(1-2): p. 37-47.
Polymenis, M. and R. Aramayo, Translate to divide: сontrol of the cell cycle by protein synthesis. Microbial cell (Graz, Austria), 2015. 2(4): p. 94-104.
Fleury, C., et al., Uncoupling protein-2: a novel gene linked to obesity and hyperinsulinemia. Nature Genetics, 1997. 15(3): p. 269-272.
Lee, Y.S., et al., Berberine, a natural plant product, activates AMP-activated protein kinase with beneficial metabolic effects in diabetic and insulin-resistant states. Diabetes, 2006. 55(8): p. 2256-64.
Klaunig, J.E., et al., Oxidative stress and oxidative damage in chemical carcinogenesis. Toxicol Appl Pharmacol, 2011. 254(2): p. 86-99.
Kohen, R. and A. Nyska, Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol, 2002. 30(6): p. 620-50.
Ramezani, J., Azarbayjani, Mohammad Ali, and M. Peeri, The Aerobic Training and Berberine Chloride Intervention on Pancreatic Tissue Antioxidant Enzymes and Lipid Peroxidation in Type 1 Diabetic Rats %J Iranian Journal of Diabetes and Obesity. 2019. 11(4): p. 257-264.
Oboh, G., A.J. Akinyemi, and A.O. Ademiluyi, Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie, 2012. 64(1-2): p. 31-36.
Kulkarni, S.K. and A. Dhir, Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Phytother Res, 2010. 24(3): p. 317-24.
Wood, J.D., et al., Effects of fatty acids on meat quality: a review. Meat Science, 2004. 66(1): p. 21-32.
Gündüz, N. and Y. Oznurlu, Adverse effects of aflatoxin B1 on skeletal muscle development in broiler chickens. British Poultry Science, 2014. 55(5): p. 684-692.
Gu, M., et al., Effects of Berberine on Cell Cycle, DNA, Reactive Oxygen Species, and Apoptosis in L929 Murine Fibroblast Cells. Evid Based Complement Alternat Med, 2015. 2015: p. 796306.
Li, Z., et al., Antioxidant and anti-inflammatory activities of berberine in the treatment of diabetes mellitus. 2014. 2014.
Goliomytis, M., et al., The Influence of Naringin or Hesperidin Dietary Supplementation on Broiler Meat Quality and Oxidative Stability. PLoS One, 2015. 10(10): p. e0141652.
Sarna, L.K., et al., Berberine inhibits NADPH oxidase mediated superoxide anion production in macrophages. Can J Physiol Pharmacol, 2010. 88(3): p. 369-78.
Furukawa, S., et al., Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest, 2004. 114(12): p. 1752-61.
Cheng, F., et al., Berberine improves endothelial function by reducing endothelial microparticles-mediated oxidative stress in humans. International Journal of Cardiology, 2013. 167(3): p. 936-942.
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