The prevalence of Vibrio Cholera Infection among Patients with Diarrheal Infections in flood affected population of Pol-e Dokhtar County: 2019
Archives of Medical Laboratory Sciences,
Vol. 5 No. 2 (2019),
20 March 2019
,
Page 1-3
https://doi.org/10.22037/amls.v5i2.29313
Abstract
Backgrounds: Cholera is an acute diarrheal intestinal infection caused by infection of intestine with vibrio cholera. Cholera is defined as endemic in Iran, thus rapid detection in the early stage of infection is crucial, especially during the disease outbreaks.
Materials and Methods: We collected 172 rectal swab samples from patients with diarrheal infections, and sterile beakers were used to collect water samples from some regions of Pol-e Dokhtar city. The collected specimens were transported to the laboratory using Cary-Blair transport medium and were then grown and incubated on alkaline peptone water (APW) and thiosulfate citrate bile salts sucrose (TCBS) agar. Then, all colonies that grew in Kligler Iron Agar (KIA) were cultured.
Results: In this study, colonies were grown on APW and TCBS media, and were then transferred to the KIA. None of the colonies were shown to be belonged to V. cholerae strains. Thus, all collected samples were negative for V. cholerae in all patients as well as the drinking water in most regions of the city.
Conclusion: The findings of this study suggest that V. cholerae was not the major cause of severe diarrheal infections in a flood-hit population of Pol-e Dokhtar city, and therefore other bacterial and viral agents should be responsible for severe diarrheal illnesses in this population.
- Vibrio cholera
- diarrheal infections
- flood affected population
- Pol-e Dokhtar
How to Cite
References
Goodarzi H, Nassirabady N, Mehdey Nezhad M. Identification of vibrio cholera O1 (vibrionaceae) in Karoon river of Ahvaz. Jentashapir journal of health research 2011;2(2):85-90.
Rahbar Taramsari M, Khosh Amooz H, Hoseini S, Badsar A.R, Vanak Araghiyan A, Falah Karkan M. Antibiotic Resistance Pattern of Cholera Epidemy in Guilan Province. Journal of Guilan University of Medical Sciences 2011,21(82): 24-30.
Rahbar M, Saburian R, Sarami M, Abbasi M, Masoumi-asl M, Sorosh M. survey the aspects of epidemiology and antibiotic resistance of Vibrio cholera eltor serotype, Inaba biotype in Iran summer epidemy 2005. Ardabil med j 2007; 1(7): 41- 45.
Akbari M R, Ahmadi A, Salimian J. Evaluation of Antibodies Produced against Lipopolysaccharides of Vibrio cholerae for Rapid Detection of Cholera. Pathobiology Research 2016: 18 (4): 23-31.
Mirnejad R , Shoja Sh, Fooladi AI . Use of a Multiplex PCR assay for simultaneous detection of the ctxA,ctxB and zot genes of Vibrio cholerae isolated from patients. Iran J Med Microbiol 2013; 7(1): 22-27.
Vanden Broeck D, Horvath C, De Wolf MJ. Vibrio cholerae: cholera toxin. Int J Biochem Cell Biol 2007; 39(10):1771-5.
Sharifnia A, Bakhshi B, Hashemi M, Porshafie. molecular survey of Seroepidemiology of cholera in Iran during a 5-year study. Islamic azad Med j 2010; 4(20):252-257.
Shimeda T, Arakawa E. Extended serotyping scheme for Vibrio cholerae. Curr Microbiol 1994; 28: 175-78.
Yamamato T, Nair GB. Survey of in vitro susceptibilities of Vibrio cholerae O1 and O139 to antimicrobial agents.Antimicrob. Agents Chemother 1995; 39: 241-44.
Faruque SM, Albert MJ, Mekalanos JJ. Epidemiology, genetics, and ecology of toxigenic Vibrio cholerae. Microbiol Mol Biol Rev 1998;62(4):1301-14.
Dick MH, Guillerm M, Moussy F, Chaignat CL. Review of two decades of cholera diagnostics how far have we really come?PLoS Negl Trop Dis 2012;6(10):e1845.
Bakhshi B, Naseri H, Zahraei SM, Afshani M, Pourshafie MR. Report on Clonal Dissemination of a New Vibrio choleraeSerotype O1 Biotype El Tor Strain in Kurdstan in Summer of 2007. Iran J Med Microbiol 2007;1(2): 67-69.
- Abstract Viewed: 117 times
- pdf Downloaded: 65 times