Probing the Biological evaluations of a new designed Palladium (II) complex using spectroscopic and theoretical approaches: Human Hemoglobin as a Target
Archives of Medical Laboratory Sciences,
Vol. 3 No. 3 (2017),
13 May 2018
https://doi.org/10.22037/amls.v3i3.21712
Abstract
Background: Previous studies reported that Palladium (Pd)(II) drug compounds showed significant anti-tumor activity in comparison with cis-platin.
Materials and Methods: In this study, we investigated the biological evaluations of a designed Pd (II) complexes (bi pyridine ethyl dithiocarbamate palladium II nitrate) via its anti-proliferative effects on the alterations in the function and structure of human hemoglobin (Hb) at different temperatures of 25 and 37°. Also for further investigation, multi-spectroscopic methods such as fluorescence and the far-UV circular dichroism (CD) with hemoglobin target were assessed.
Results: Fluorescence data showed the pure ability of Pd(II) complex to quench the intrinsic fluorescence of Hb. The binding constant, number of binding sites, and thermodynamic parameters at two temperatures were assessed and the results demonstrated the major possibility of occurring electrostatic and hydrophobic interactions in the Pd (II) complex–Hb interaction. For evaluating the change of secondary structure of Hb upon interaction with various concentrations of complex, far-UV CD spectra was applied and it was observed that in high dose of complex, significant changes occurred which is indicative of some side effects in overdosing of this complex.
Conclusion: Our results suggested that using palladium complex as an anticancer agent might cause some disorders in structure and function of Hb as well as improve understanding of the side effects of newly designed metal anticancer drugs.
- Palladium complexes
- Hemoglobin
- Chemotherapy
- Quenching
How to Cite
References
Emami S, Ghourchian H, Divsalar A. Release of Cyt c from the model membrane due to conformational change induced by anticancer palladium complex. International Journal of Biological Macromolecules. 2011;48(2),243-8.
Warad I, Eftaiha AF, Al-Nur MA, Husein AI, Assal M, Abu-Obaid A, et al. Metal ions as Antitumor Complexes-Review. Journal of Materials and Environmental Science. 2013;4(4)542-57.
Cristina Campanella N, da Silva Demartini M, Torres C, Tonon de Almeida E, Marli Cação Paiva Gouvêa C. The cytotoxic and growth inhibitory effects of palladium (II) complexes on MDA-MB-435 cells. Genetics and Molecular Biology. 2012;35(1),159-63.
Mansouri Torshizi H. Saeidifar M, Divsalar A, Saboury AA. Study on Interaction of DNA from Calf Thymus with 1,10 phenanthroline hexyl dithio carbamato palladium(II) nitrate as Potential Antitumor Agent. Journal of Biomolecular Structure & Dynamics. 2011;28(5),805-14.
Melber C, Keller D, Mangelsdorf I. Environmental Health Criteria 226 Palladium, the International Labour Organization or the World Health Organization. 2002;35,14-117.
Cohen GM. Caspases: the executioners of apoptosis. Biochem Journal. 1997;326(Pt1),1-16.
Safo MK, Ahmed MH, Ghatge MS, Boyiri T. Hemoglobin–ligand binding: Understanding Hb function and allostery on atomic level. Biochimica et Biophysica Acta (BBA)–Proteins and Proteomics. 2011;1814(6),797-809.
Mousavy SJ, Riazi GH, Kamarei M, Aliakbarian H, Sattarahmady N, Sharifizadeh A, et al. Effects of mobile phone radio frequency on the structure and function of the normal human hemoglobin. International Journal of Biological Macromolecules. 2009;44(3),278-85.
Ding F, Han BY, Liu W, Zhang L, Sun Y. Interaction of imidacloprid with hemoglobin by fluorescence and circular dichroism. Journal of Fluorescence. 2010;20(3),753-62.
Perutz MF, Fermi G, Abraham DJ, Poyart C, Bursaux E. Hemoglobin as a receptor of drugs and peptides: x-ray studies of the stereochemistry of binding. Journal of the American Chemical Society. 1986;108(5),1064-78.
Peter HS, Harry PJMD. Direct determination of absolute circular dichroism data and calibration of commercial instrument. Analytical Chemistry. 1981;53(6),778-82.
Stryer, L. Intramolecular resonance transfer of energy in proteins. Biochimica et Biophysica Acta. 1959;35,242-4.
Taheri-Kafrani A, Asgari-Mobarakeh E, Bordbar AK, Haertlé T. Structure-function relationship of beta-lactoglobulin in the presence of dodecyltrimethyl ammonium bromide. Colloids and Surfaces B: Biointerfaces. 2010;75(1),268-74.
Ghalandari B, Divsalar A, Saboury AA, Parivar K. β-Lactoglobulin nanoparticle as a chemotherapy agent carrier for oral drug delivery system. Journal of the Iranian Chemical Society. 2015;12(4),613-19.
De S, Girigoswami A. A fluorimetric and circular dichroism study of hemoglobin—Effect of pH and anionic amphiphiles Journal of Colloid and Interface Science. 2006;296(1),324-31.
Sun Y, Wei S, Yin C, Liu L, Hu C, Zhao Y, et al. Fan. Synthesis and spectroscopic characterization of 4-butoxyethoxy-N-octadecyl-1,8-naphthalimide as a new fluorescent probe for the determination of proteins. Bioorganic & Medicinal Chemistry Letters. 2011;21(12),3798-804.
Ghali M. static quenching of bovine serum albumin conjugated with small size CdS nanocrystalline quantum dots. Journal of Luminescence. 2010;130(7),1254-7.
Zhang MF, Xu ZQ, Ge YS, Jiang FL, Liu Y. Binding of fullerol to human serum albumin: spectroscopic and electrochemical approach. Journal of Photochemistry and Photobiology B: Biology. 2012; 108, 34-43.
Tabassum S, Al-Asbahy WM, Afzal M, Arjmand F. Synthesis, characterization and interaction studies of copper based drug with Human Serum Albumin (HSA): Spectroscopic and molecular docking investigations. Journal of Photochemistry and Photobiology B: Biology. 2012;114,132-9.
Divsalar A, Saboury AA, Ahadi L, Zemanatyar E, Mansoori-Torshizi H. Investigation of effects of newly synthesized Pt(II) complex against human serum albumin and leukemia cell line of K562. BMB Reports. 2010;43(11),766-71.
Hu YJ, Yue HL, Li XL, Zhang SS, Tang E, Zhang LP. Molecular spectroscopic studies on the interaction of morin with bovine serum albumin. Journal of Photochemistry and Photobiology B: Biology. 2012;112,16-22.
Faridbod F, Ganjali MR, Larijani B, Riahi S, Saboury AA, Hosseinie M, et al. Spectrochim. Interaction study of pioglitazone with albumin by fluorescence spectroscopy and molecular docking. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2011;78(1),96-101.
He XM, Carter DC. Atomic structure and chemistry of human serum albumin. Nature. 1992;358(6383),209-15.
Yang JT, Wu CSC, Martinez HM. Calculation of protein conformation from circular dichroism. Methods in Enzymology. 1986;130,208-269.
Yang L, Huo D, Hou C, Yang M, Fa H, Luo X. Interaction of monosulfonate tetraphenyl porphyrin (H2TPPS1) with plant-esterase: Determination of the binding mechanism by spectroscopic methods. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy . 2011;78(5),1349-55.
Hu YJ, Liu Y, Zhao RM, Qu SS. Interaction of colchicine with human serum albumin investigated by spectroscopic methods. International Journal of Biological Macromolecules. 2005;37(3),122-126.
Xi J, Guo R. Interactions between flavonoids and hemoglobin in lecithin liposomes. International Journal of Biological Macromolecules. 2007;40(4),305-11
Divsalar A, Saboury AA, Yousefi R, Moosavi-Movahedi AA, Mansoori-Torshizi H. Spectroscopic and cytotoxic studies of the novel designed palladium(II) complexes: beta lactoglobulin and K562 as the targets. Int. International Journal of Biological Macromolecules. 2007;40(4),381-6.
Marques MPM, Platinum and Palladium Polyamine Complexes as Anticancer Agents: The Structural Factor. Hindawi Publishing Corporation. 2013; 287353,29P.
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