Up-regulation of MiR-21 in Oral Squamous Cell Carcinoma

Mona Peyman, Hassan Mir Mohammad Sadeghi, Hakimeh Zali, Yousef Arianmehr, Solmaz Alihosseini, Farzad Yazdani, Saeed Hesami Tackallou

Abstract


85

Introduction:

Oral squamous cell carcinoma (OSCC) is the most abundant dysplasia in the oral cavity that aberrant expression of microRNA plays an important role in cancer progression. It is well known that microRNA-21 expression is oncogenes or tumor suppressor factor in malignancy formation of several cancers. In this study used OSCC tissues to investigate the expression of mir-21 in malignancy causes.

Material and Methods:

OSCC tissues and normal samples were collected from Amir Alam and Taleghani Hospitals as fresh and frozen samples. The Expression level of miR-21 in OSCC and normal tissues were measured by qRT-PCR. To find the targets related mir-21 used databases including TargetScan, GenTrail2, GO and STITCH online websites.

Results:

MiR-21 found significantly up-regulated in OSCC tissues compared to normal tissues (Fold-change=5.54). Targets of miR-21 derived from the TargetScan and GeneTrail2 analysis determined the most significant biological processes be associated with the epithelialization, differentiation, and morphogenesis. So overexpression of miR-21 could reverse this process and promote the cells to stemness and metastatic state. EGFR and PDCD4, two targets of miR-21, previously have been demonstrated that are important in OSCC invasion, metastasis, and differentiation. In addition study on targets genes to find anticancer drugs enriched CI-1033 as a tyrosine-kinase inhibitor that previously reported for the treatment of cancer.

Conclusion:

Our findings indicate miR-21 act as oncomiR in OSCC and may be considered as a biomarker for the development of OSCC treatment.


Keywords


Oral squamous cell carcinoma (OSCC), miR-21, qRT-PCR, Bioinformatics

Full Text:

PDF

40

References


Mork J, Lie AK, Glattre E, Clark S, Hallmans G, Jellum E, Koskela P, Møller B, Pukkala E, Schiller JT, Wang Z. Human papillomavirus infection as a risk factor for squamous-cell carcinoma of the head and neck. New England Journal of Medicine. 2001 Apr 12;344(15):1125-31.

Jia LF, Wei SB, Gong K, Gan YH, Yu GY. Prognostic implications of micoRNA miR-195 expression in human tongue squamous cell carcinoma. PLoS One. 2013 Feb 22;8(2):e56634.

Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. cell. 2011 Mar 4;144(5):646-74.

Pauli A, Rinn JL, Schier AF. Non-coding RNAs as regulators of embryogenesis. Nature Reviews Genetics. 2011 Feb;12(2):136.

Kolokythas A, Miloro M, Zhou X. Review of MicroRNA proposed target genes in oral cancer. Part II. Journal of oral & maxillofacial research. 2011 Apr;2(2).

Stöckel D, Kehl T, Trampert P, Schneider L, Backes C, Ludwig N, Gerasch A, Kaufmann M, Gessler M, Graf N, Meese E. Multi-omics enrichment analysis using the GeneTrail2 web service. Bioinformatics. 2016 Jan 18;32(10):1502-8.

Guo J, Wang M, Liu X. MicroRNA-195 suppresses tumor cell proliferation and metastasis by directly targeting BCOX1 in prostate carcinoma. Journal of Experimental & Clinical Cancer Research. 2015 Dec;34(1):91.

C Cheng YS, Rees T, Wright J. A review of research on salivary biomarkers for oral cancer detection. Clinical and translational medicine. 2014 Dec 1;3(1):3.

Hedbäck N, Jensen DH, Specht L, Fiehn AM, Therkildsen MH, Friis-Hansen L, Dabelsteen E, von Buchwald C. MiR-21 expression in the tumor stroma of oral squamous cell carcinoma: an independent biomarker of disease free survival. PloS one. 2014 Apr 22;9(4):e95193.

Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C T method. Nature protocols. 2008 Jun;3(6):1101.

Henrique T, José Freitas da Silveira N, Henrique Cunha Volpato A, Mioto MM, Carolina Buzzo Stefanini A, Bachir Fares A, Gustavo da Silva Castro Andrade J, Masson C, Verónica Mendoza López R, Daumas Nunes F, Paulo Kowalski L. HNdb: an integrated database of gene and protein information on head and neck squamous cell carcinoma. Database. 2016 Jan 1;2016.

Min A, Zhu C, Peng S, Rajthala S, Costea DE, Sapkota D. MicroRNAs as important players and biomarkers in oral carcinogenesis. BioMed research international. 2015;2015.

Seike M, Goto A, Okano T, Bowman ED, Schetter AJ, Horikawa I, Mathe EA, Jen J, Yang P, Sugimura H, Gemma A. MiR-21 is an EGFR-regulated anti-apoptotic factor in lung cancer in never-smokers. Proceedings of the National Academy of Sciences. 2009 Jul 21;106(29):12085-90.

Frankel LB, Christoffersen NR, Jacobsen A, Lindow M, Krogh A, Lund AH. Programmed cell death 4 (PDCD4) is an important functional target of the microRNA miR-21 in breast cancer cells. Journal of Biological Chemistry. 2007 Nov 8.

Garofalo M, Romano G, Di Leva G, Nuovo G, Jeon YJ, Ngankeu A, Sun J, Lovat F, Alder H, Condorelli G, Engelman JA. EGFR and MET receptor tyrosine kinase–altered microRNA expression induces tumorigenesis and gefitinib resistance in lung cancers. Nature medicine. 2012 Jan;18(1):74.

Li J, Huang H, Sun L, Yang M, Pan C, Chen W, Wu D, Lin Z, Zeng C, Yao Y, Zhang P. MiR-21 indicates poor prognosis in tongue squamous cell carcinomas as an apoptosis inhibitor. Clinical cancer research. 2009 Jun 15;15(12):3998-4008.

Allen LF, Lenehan PF, Eiseman IA, Elliott WL, Fry DW. Potential benefits of the irreversible pan-erbB inhibitor, CI-1033, in the treatment of breast cancer. InSeminars in oncology 2002 Jun 1 (Vol. 29, No. 3, pp. 11-21). WB Saunders.

Slichenmyer WJ, Elliott WL, Fry DW. CI-1033, a pan-erbB tyrosine kinase inhibitor. InSeminars in oncology 2001 Oct 1 (Vol. 28, pp. 80-85). WB Saunders.




DOI: https://doi.org/10.22037/rrr.v2i3.22368

Refbacks

  • There are currently no refbacks.


ISSN:2476-5163 (Print); 2476-5171 (Online)

 
Creative Commons LicenseThe Journal of "Regeneration, Reconstruction, & Restoration" is licensed under a Creative Commons Attribution 4.0 International License.