Enriched Satellite Cells with Pre-plate Technique Differentiate Strongly on Electrospun Polyacrylonitril Membrane

Simzar Hosseinzadeh, Masoud Soleimani

Abstract


235

Introduction: Satellite cells known as the main regenerating cell types in skeletal muscle which can be isolated using pre-plate technique due to weak or slow adhesive interactions with satellite cells. Although, there are some issues about digestion of muscle tissue and isolation of satellite cells, which highlight need for an efficient procedure. Also, the employment of a nanofibrous surface can facilitate the attachment of satellite cells to reach matured muscle tissue. On the other hand, polyacrylonitrile (PAN) has been reported as a biocompatible polymer that can be electrospun into a nonwoven membrane. Materials and Methods: Herein, a modified digestion and pre-plate protocol was established for the enrichment of satellite cells. Also, a PAN electrospun scaffold was used to provide a higher surface area for cell attachment compared to tissue culture polystyrene (TCPS). However, the surface of prepared scaffold was modified with plasma treatment to progress cell adhesion. Results: The corresponding scaffold was examined with scanning electron microscopy (SEM) and tensile examination. The enriched cells, which exhibited a close gene expression pattern with satellite cells, seeded on this electrospun PAN membrane. The cultured satellite cells showed a good tendency to surface of PAN scaffold and also a higher rate of cell proliferation. Subsequently, the cells were induced to more expression of specific muscle genes compared to TCPS group. Conclusion: As a whole, satellite cells could mature to multinuclear cells using PAN scaffold as a function of efficient mechanical property and also higher surface area. 


Keywords


Polyacrylonitrile; Electrospinning; Skeletal muscle; Satellite cells; Pre-plate technique; Stem cells

Full Text:

PDF

112

References


Cai D, Lee KK, Li M, Tang MK, Chan KM. Ubiquitin expression is up-regulated in human and rat skeletal muscles during aging. Arch Biochem Biophys. 2004;425(1):42-50.

Riboldi SA, Sampaolesi M, Neuenschwander P, Cossu G, Mantero S. Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineering. Biomaterials. 2005;26(22):4606-15.

Sacco A, Doyonnas R, Kraft P, Vitorovic S, Blau HM. Self-renewal and expansion of single transplanted muscle stem cells. Nature. 2008;456(7221):502-6.

Usas A, Huard J. Muscle-derived stem cells for tissue engineering and regenerative therapy. Biomaterials. 2007;28(36):5401-6.

Hosseinzadeh S, Soleimani M, Rezayat SM, Ai J, Vasei M. The activation of satellite cells by nanofibrous poly ɛ-caprolacton constructs. Journal of Biomaterials Applications. 2014;28(6):801-12.

Yiou R, Lefaucheur JP, Atala A. The regeneration process of the striated urethral sphincter involves activation of intrinsic satellite cells. Anat Embryol (Berl). 2003;206(6):429-35.

Hawke TJ, Garry DJ. Myogenic satellite cells: physiology to molecular biology. J Appl Physiol. 2001;91(2):534-51.

Siegel AL, Atchison K, Fisher KE, Davis GE, Cornelison DD. 3D timelapse analysis of muscle satellite cell motility. Stem Cells. 2009;27(10):2527-38.

Peng H, Huard J. Muscle-derived stem cells for musculoskeletal tissue regeneration and repair. Transpl Immunol. 2004;12(3-4):311-9.

Arsic N, Mamaeva D, Lamb NJ, Fernandez A. Muscle-derived stem cells isolated as non-adherent population give rise to cardiac, skeletal muscle and neural lineages. Exp Cell Res. 2008;314(6):1266-80.

Otto A, Collins-Hooper H, Patel K. The origin, molecular regulation and therapeutic potential of myogenic stem cell populations. J Anat. 2009;215(5):477-97.

Peter S. Zammit TAP, and Zipora Yablonka-Reuveni. The Skeletal Muscle Satellite Cell: The Stem Cell That Came in

From the Cold. Journal of Histochemistry & Cytochemistry. 2006;54(11): 1177–91.

Ieronimakis N, Balasundaram G, Rainey S, Srirangam K, Yablonka-Reuveni Z, Reyes M. Absence of CD34 on murine skeletal muscle satellite cells marks a reversible state of activation during acute injury. PLoS One. 2010;5(6):e10920.

Bach A, Beier J, Stern‐Staeter J, Horch R. Skeletal muscle tissue engineering. Journal of cellular and molecular medicine. 2004;8(4):413-22.

Hosseinzadeh S, Mahmoudifard M, Mohamadyar-Toupkanlou F, Dodel M, Hajarizadeh A, Adabi M, et al. The nanofibrous PAN-PANi scaffold as an efficient substrate for skeletal muscle differentiation using satellite cells. Bioprocess and biosystems engineering. 2016;39(7):1163-72.

Hosseinzadeh S, Esnaashari S, Sadeghpour O, Hamedi S. Predictive modeling of phenolic compound release from nanofibers of electrospun networks for application in periodontal disease. Journal of Polymer Engineering. 2016;36(5):457-64.

Saeed K, Haider S, Oh T-J, Park S-Y. Preparation of amidoxime-modified polyacrylonitrile (PAN-oxime) nanofibers and their applications to metal ions adsorption. Journal of Membrane Science. 2008;322(2):400-5.

Giri Dev VR, Venugopal JR, Senthilkumar M, Gupta D, Ramakrishna S. Prediction of water retention capacity of hydrolysed electrospun polyacrylonitrile fibers using statistical model and artificial neural network. Journal of Applied Polymer Science. 2009;113(5):3397-404.

Dadvar S, Tavanai H, Morshed M. Fabrication of nanocomposite PAN nanofibers containing MgO and Al2O3 nanoparticles. Polymer Science Series A. 2014;56(3):358-65.

Jeong SI, Jun ID, Choi MJ, Nho YC, Lee YM, Shin H. Development of Electroactive and Elastic Nanofibers that contain Polyaniline and Poly (L‐lactide‐co‐ε‐caprolactone) for the Control of Cell Adhesion. Macromolecular bioscience. 2008;8(7):627-37.

Michelle I. Smith YYH, Mohanish Deshmukh. Skeletal Muscle Differentiation Evokes Endogenous XIAP

to Restrict the Apoptotic Pathway. PLoS One. March 2009;4(3).

Garry DJ, Olson EN. A common progenitor at the heart of development. Cell. 2006;127(6):1101-4.

Baharvand H, Mehrjardi NZ, Hatami M, Kiani S, Rao M, Haghighi MM. Neural differentiation from human embryonic stem cells in a defined adherent culture condition. Int J Dev Biol. 2007;51(5):371-8.

Mahmoudifard M, Soleimani M, Hatamie S, Zamanlui S, Ranjbarvan P, Vossoughi M, et al. The different fate of satellite cells on conductive composite electrospun nanofibers with graphene and graphene oxide nanosheets. Biomedical Materials. 2016;11(2):025006.

Hosseinzadeh S, Rezayat SM, Vashegani-Farahani E, Mahmoudifard M, Zamanlui S, Soleimani M. Nanofibrous hydrogel with stable electrical conductivity for biological applications. Polymer. 2016;97:205-16.

Zammit PS, Relaix F, Nagata Y, Ruiz AP, Collins CA, Partridge TA, et al. Pax7 and myogenic progression in skeletal muscle satellite cells. J Cell Sci. 2006;119(Pt 9):1824-32.

Morgan JE, Partridge TA. Muscle satellite cells. Int J Biochem Cell Biol. 2003;35(8):1151-6.

Hawke TJ, Atkinson DJ, Kanatous SB, Van der Ven PF, Goetsch SC, Garry DJ. Xin, an actin binding protein, is expressed within muscle satellite cells and newly regenerated skeletal muscle fibers. Am J Physiol Cell Physiol. 2007;293(5):C1636-44.

Pavlath GK. Spatial and functional restriction of regulatory molecules during mammalian myoblast fusion. Exp Cell Res. 2010;316(18):3067-72.

Hollnagel A, Grund C, Franke WW, Arnold HH. The cell adhesion molecule M-cadherin is not essential for muscle development and regeneration. Mol Cell Biol. 2002;22(13):4760-70.

Sinanan AC, Buxton PG, Lewis MP. Muscling in on stem cells. Biol Cell. 2006;98(4):203-14.




DOI: https://doi.org/10.22037/rrr.v2i1.17614

Refbacks

  • There are currently no refbacks.


ISSN:2476-5163 (Print); 2476-5171 (Online)

 
Creative Commons LicenseThe Journal of "Regeneration, Reconstruction, & Restoration" is licensed under a Creative Commons Attribution 4.0 International License.