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Contribution of NOTCH signaling pathway along with TNF-? in the intestinal inflammation of ulcerative colitis

Mahsa Ghorbaninejad, Raheleh Heydari, Parvaneh Mohammadi, Shabnam Shahrokh, Mehrdad Haghazali, Binazir Khanabadi, Anna Meyfour




Aim: The aim of this study was to determine gene expression levels of TNF-?, NOTCH1 and HES1 in patients with UC.

Background: Intestinal inflammation and epithelial injury are the leading actors of inflammatory bowel disease (IBD), causing an excessive pro-inflammatory cytokines expression such as TTNF-?. Also, target genes of NOTCH signaling are involved in the regulation of intestinal homeostasis. Previous studies demonstrated that TNF-? increases in ulcerative colitis (UC) patients but the relationship between TNF-? and NOTCH signaling pathway in UC etiopathology needs to further study.  

Methods:  Twelve active UC patients and twelve healthy controls were enrolled into this study. RNA was extracted and the mRNA expression levels of TNF-?, NOTCH1 and HES1 were examined using real-time PCR analyses. Moreover, transcriptome data deposited in Gene Expression Omnibus (GEO) database were analyzed to detect the differential expression of TNF superfamily and NOTCH1gene in IBD patients. Finally, the interaction of TNF-? and NOTCH signaling was obtained from The SIGnaling Network Open Resource 2.0 (SIGNOR 2.0) database.

Results: The transcription level of TNF-?, NOTCH1 and HES1 genes were significantly increased in UC patients compared with control (p < 0.05). In addition, GEO results confirmed our expression results. SIGNOR analysis showed TNF-? interacts with NOTCH signaling components.

Conclusion: Based on our data, we showed that NOTCH1 and HES1 in co-operation of TNF-?, may play an important role in pathogenesis of UC. The members of NOTCH signaling pathway can be ideal candidates to target therapy of IBD.


Ulcerative colitis, TNF-?, NOTCH signaling, HES1



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DOI: https://doi.org/10.22037/ghfbb.v12i0.1821