Comparative Study of Gastric Cancer and Chronic Gastritis via Network Analysis
Aim: In this study the significant differentially expressed genes (DEGs) related to gastric cancer (GC) and chronic gastritis were
screened to introduce common and distinctive genes between the two diseases.
Background: Diagnosis of gastric cancer as a mortal disease and chronic gastritis the stomach disorder which can be considered as
risk factor of GCs required safe and effective molecular biomarkers.
Methods: Microarray profiles were downloaded from Gene Expression Omnibus (GEO) and analyzed via GEO2R. The candidate
DEGs plus relevant genes from STRING database were interacted by Cytoscape software version 3.6.0 the central nodes were
determined and analyzed.
Results: JUN, GAPDH, FOS, TP53, PRDM10, VEGFA, and CREB1 as central nodes and TFF1 and ERG1 as the top changed
expressed genes were determined as critical nodes related to gastric cancer. GAPDH, PRDM10, TP53, JUN, AKT1, EGFR, MAPK1,
EGF, DECR1, and MYC were identified as common remarkable genes between GC and chronic gastritis.
Conclusion: Identification of distinctive and common genes between GC and chronic gastritis can be useful in the early stage
detection of disease and reducing risk of GCs.
Keywords: Chronic gastritis, Gene ontology, Biomarkers, Gastric cancer.
(Please cite as: Mansouri V, Rezaei Tavirani S, Zadeh-Esmaeel MM, Rostami-Nejad M, Rezaei-Tavirani M.
Comparative study of gastric cancer and chronic gastritis via network analysis. Gastroenterol Hepatol Bed
Ge S, Xia X, Ding C, Zhen B, Zhou Q, Feng J, et al. A proteomic landscape of diffuse-type gastric cancer. Nature communications. 2018;9(1):1012.
Yoo MW, Park J, Han HS, Yun YM, Kang JW, Choi DY, et al. Discovery of gastric cancer specific biomarkers by the application of serum proteomics. Proteomics. 2017;17(6):1600332.
Sipponen P, Maaroos H-I. Chronic gastritis. Scandinavian journal of gastroenterology. 2015;50(6):657-67.
Rugge M, Meggio A, Pravadelli C, Barbareschi M, Fassan M, Gentilini M, et al. Gastritis staging in the endoscopic follow-up for the secondary prevention of gastric cancer: a 5-year prospective study of 1755 patients. Gut. 2018:gutjnl-2017-314600.
Emura F, Gralnek I, Sano Y, Baron TH. Improving early detection of gastric cancer: a novel systematic alphanumeric-coded endoscopic approach. Revista de Gastroenterología del Perú. 2017;33(1):52-8.
Wulfkuhle JD, Liotta LA, Petricoin EF. Early detection: proteomic applications for the early detection of cancer. Nature reviews cancer. 2003;3(4):267.
Safari-Alighiarloo N, Taghizadeh M, Rezaei-Tavirani M, Goliaei B, Peyvandi AA. Protein-protein interaction networks (PPI) and complex diseases. Gastroenterology and Hepatology from bed to bench. 2014;7(1):17.
Zamanian–Azodi M, Rezaei–Tavirani M, Hasanzadeh H, Rad SR, Dalilan S. Introducing biomarker panel in esophageal, gastric, and colon cancers; a proteomic approach. Gastroenterology and Hepatology from bed to bench. 2015;8(1):6.
Rezaei-Tavirani M, Rezaei-Tavirani M, Mansouri V, Mahdavi SM, Valizadeh R, Rostami-Nejad M, et al. Introducing crucial protein panel of gastric adenocarcinoma disease. Gastroenterology and Hepatology from bed to bench. 2017;10(1):21.
Azadegan-Dehkordi F, Bagheri N, Shirzad M, Sanei MH, Hashemzadeh-Chaleshtori M, Rafieian-Kopaei M, et al. Correlation between mucosal IL-6 mRNA expression level and virulence factors of Helicobacter pylori in Iranian adult patients with chronic gastritis. Jundishapur journal of microbiology. 2015;8(8).
Rahimian G, Sanei MH, Shirzad H, Azadegan-Dehkordi F, Taghikhani A, Salimzadeh L, et al. Virulence factors of Helicobacter pylori vacA increase markedly gastric mucosal TGF-β1 mRNA expression in gastritis patients. Microbial pathogenesis. 2014;67:1-7.
Tavirani MR, Mansouri V, Tavirani SR, Tackallou SH, Nejad MR. Gliosarcoma Protein-Protein Interaction Network Analysis and Gene Ontology. International Journal of Cancer Management. 2018;11(5).
Van't Veer LJ, Dai H, Van De Vijver MJ, He YD, Hart AA, Mao M, et al. Gene expression profiling predicts clinical outcome of breast cancer. nature. 2002;415(6871):530.
Buache E, Etique N, Alpy F, Stoll I, Muckensturm M, Reina-San-Martin B, et al. Deficiency in trefoil factor 1 (TFF1) increases tumorigenicity of human breast cancer cells and mammary tumor development in TFF1-knockout mice. Oncogene. 2011;30(29):3261.
Park SY, Kim JY, Lee SM, Chung JO, Lee KH, Jun CH, et al. Expression of early growth response gene‑1 in precancerous lesions of gastric cancer. Oncology letters. 2016;12(4):2710-5.
Vazquez A, Flammini A, Maritan A, Vespignani A. Global protein function prediction from protein-protein interaction networks. Nature biotechnology. 2003;21(6):697.
Tzouvelekis A, Harokopos V, Paparountas T, Oikonomou N, Chatziioannou A, Vilaras G, et al. Comparative expression profiling in pulmonary fibrosis suggests a role of hypoxia-inducible factor-1α in disease pathogenesis. American journal of respiratory and critical care medicine. 2007;176(11):1108-19.
Jongeneel CV, Delorenzi M, Iseli C, Zhou D, Haudenschild CD, Khrebtukova I, et al. An atlas of human gene expression from massively parallel signature sequencing (MPSS). Genome research. 2005;15(7):1007-14.
Alexander MC, Lomanto M, Nasrin N, Ramaika C. Insulin stimulates glyceraldehyde-3-phosphate dehydrogenase gene expression through cis-acting DNA sequences. Proceedings of the National Academy of Sciences. 1988;85(14):5092-6.
Chajès V, Cambot M, Moreau K, Lenoir GM, Joulin V. Acetyl-CoA carboxylase α is essential to breast cancer cell survival. Cancer research. 2006;66(10):5287-94.
Rezaei-Tavirani M, Moghaddamnia SH, Ranjbar B, Amani M, Marashi S-A. Conformational study of human serum albumin in pre-denaturation temperatures by differential scanning calorimetry, circular dichroism and UV spectroscopy. BMB Reports. 2006;39(5):530-6.
Fog CK, Galli GG, Lund AH. PRDM proteins: important players in differentiation and disease. Bioessays. 2012;34(1):50-60.
Lim JY, Yoon SO, Hong SW, Kim JW, Choi SH, Cho JY. Thioredoxin and thioredoxin-interacting protein as prognostic markers for gastric cancer recurrence. World journal of gastroenterology: WJG. 2012;18(39):5581.
Kong WQ, Bai R, Liu T, Cai CL, Liu M, Li X, et al. MicroRNA‐182 targets cAMP‐responsive element‐binding protein 1 and suppresses cell growth in human gastric adenocarcinoma. The FEBS journal. 2012;279(7):1252-60.
Jia H, Bagherzadeh A, Bicknell R, Duchen MR, Liu D, Zachary I. Vascular endothelial growth factor (VEGF)-D and VEGF-A differentially regulate KDR-mediated signaling and biological function in vascular endothelial cells. Journal of Biological Chemistry. 2004;279(34):36148-57.