Characterization of the “a” determinant Region of the Hepatitis B Virus genome in Iranian Patients at Different clinical phases of Chronic Infection
Aim: To determine the distribution of important mutations of the “a” determinant region in the HBV genome among patients in different
clinical phases of HBV infection.
Background: Variations in Hepatitis B infection not only change the outcome of the disease but also the symptoms from which the
chronic HBV patients are suffering.
Methods: We have meticulously selected a total of 40 chronic HBV patients from four different subclasses of chronic HBV clinical
phases including immune tolerant (IT), immune active (IA), inactive carrier (IC) and hepatitis B e antigen (HBeAg)-negative (ENEG);
10 samples per each phase. Mutations of the “a” determinant region were identified using PCR-Direct sequencing method.
Results: 17 amino-acid substitutions at 12 positions inside the “a” determinant were identified in all forty samples; 3 mutations in the
IT group, 6 mutations in the IA phase, 3 mutations in the IC patients and 5 mutations in the ENEG phase. Different substitutions were
observed in all four clinical phases. The IA phase was the most variant group with the highest number of amino-acid substitutions.
Conclusion: These results did not reveal a strong pattern to distinguish different clinical phases of Chronic HBV infection, but there
are some obvious differences regarding the number and position of mutations between these four clinical phases.
Keywords: “a” determinant region, Chronic hepatitis B infection, Clinical phases, Amino-acid substitution.
(Please cite as: Romani S, Hosseini SM, Mohebbi SR, Boonstra A, Hosseini Razavi A, Sharifian A. Characterization
of the “a” determinant region of the hepatitis B virus genome in Iranian patients at different clinical phases of
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