Molecular Characterization of Shigella spp. isolates from a pediatric hospital in Southwestern Iran

Leili Shokoohizadeh, Gholam Abbas Kaydani, Alireza Ekrami

Abstract


17

Aim:  This study focused on the detection of dominant clones and genetic relationship of Shigella spp.  isolated   from children with diarrhea in the main pediatric hospital in Ahvaz by Multi-Locus Sequence Typing (MLST) technique.

Background:

 Shigellosis is considered as one of the problematic bacterial infections for public health in the world. Khuzestan province in the Southwestern part of Iran is a known endemic area for infections due to Shigella. There are limited molecular epidemiological data of Shigella spp. in this area.

Methods: A total of 50 Shigella spp. were isolated from January-June 2015 based on conventional microbiology and serology tests. The Sequence types (ST) of Shigella isolates which are characterized by Enterobacterial repetitive intergenic consensus (ERIC-PCR) were detected by MLST technique. 

                     

Results: Among 50 Shigella isolates, a total of 31(62%), 16(32%) and 3 (6%) of Shigella isolates were identified as S. flexneri, S.sonneii, and S.boydii, respectively. Two different sequence types (ST152 and ST245) were identified in Shigella isolates. ST152 was detected in S.sonnei and ST245 in S. flexneri and S. boydii isolates.

 

Conclusion:  Based on MLST data, the stable and genetically linked Shigella clones are the cause of Shigella infections in children in southwestern Iran.

Keywords: Shigella, Multi-Locus Sequence Typing, Ahvaz

 


Keywords


Shigella, Multi-Locus Sequence Typing, Ahvaz

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DOI: http://dx.doi.org/10.22037/ghfbb.v0i0.1201

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GHFBB by Gastroenterology and Liver Diseases Research Institute is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

PISSN: 2008-2258

EISSN: 2008-4234


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