Molecular Characterization of Shigella spp. isolates from a pediatric hospital in Southwestern Iran
Aim: In This study focused on the detection of dominant clones and genetic relationship of Shigella spp. isolated from children with diarrhea in the main pediatric hospital in Ahvaz by multi-locus sequence typing (MLST) technique.
Background: Shigellosis is considered as one of the problematic bacterial infections for public health in the world. Khuzestan province in the Southwestern part of Iran is a known endemic area for infections due to Shigella. There are limited molecular epidemiological data for Shigella spp. in this area.
Methods: A total of 50 Shigella spp. were isolated from January-June 2015 based on conventional microbiology and serology tests. The Sequence types (ST) of Shigella isolates which are characterized by Enterobacterial repetitive intergenic consensus (ERIC-PCR) were detected by MLST technique.
Results: Among 50 Shigella isolates, a total of 31(62%), 16(32%) and 3 (6%) of Shigella isolates were identified as S. flexneri, S.sonneii, and S.boydii, respectively. Two different sequence types (ST152 and ST245) were identified in Shigella isolates. ST152 was detected in S.sonnei and ST245 in S. flexneri and S. boydii isolates.
Conclusion: Based on MLST data, the stable and genetically linked Shigella clones are the cause of Shigella infections in children in Southwestern Iran.
Keywords: Shigella, Multi-Locus Sequence Typing, Iran.
Dekker JP, Frank KM. Salmonella, Shigella, and yersinia. Clinical Lab Medicine 2015; 35 (2):225–46.
Bardhan P, Faruque AS, Naheed A, Sack DA. Decreasing shigellosis-related deaths without Shigella spp.–specific interventions, Asia. Emerg Infect Dis. 2010; 16 (11):1718.
Lin T, Lin L, Zhang F. Review on molecular typing methods of pathogens. Open journal of medical microbiology. 2014; 4(03):147.
Maccannell D. Bacterial strain typing. Clin. Lab. Med 2013; 33: 629-650.
Wilson LA Sharp PM. Enterobacterial Repetitive Intergenic Consensus (ERIC) Sequences in Escherichia coli: Evolution and Implications for ERIC-PCR. Mol Bio Evol 2006; 23:1156–1168.
Chen Y., Zhang W., Knabel S.J. Multi-virulence-locus sequence typing identifies single nucleotide polymorphisms which differentiate epidemic clones and outbreak strains of Listeria monocytogenes. J Clin Microbiol 2007; 45, 835-846.
Maiden MC, Bygraves JA, Feil E, Morelli G, Russell JE, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant DA, Feavers IM. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences. 1998 Mar 17; 95(6):3140-5.
Pour MB, Shokoohizadeh L, Navab-Akbar FT. Analysis of Clonal Relationships among Shigella spp. Isolated from Children with Shigellosis in Ahvaz, Iran. J Paramed Sci. 2016: 14; 7(2):45-51.
Shahsavan S, Nobakht M, Rastegar-Lari A, Owlia P, Bakhshi B. Multi-locus sequence type analysis of Shigellas pp. isolates from Tehran, Iran. Iranian J Microbiol. 2016 Oct 1; 8(5).
Eftekhari N, Bakhshi B, Pourshafie MR, Zarbakhsh B,Rahbar M, Hajia M, et al. Genetic diversity of Shigella spp. and their integron content. Foodborne Pathog Dis. 2013; 10:237–242.
Ranjbar R, Dallal MMS, Talebi M, Pourshafie MR. Increased isolation and characterization of Shigella sonnei obtained from hospitalized children in Tehran, Iran. J Health Popul Nutr 2008; 26: 426.
Cao Y, Wei D, Kamara IL, Chen W. Multi-Locus Sequence Typing (MLST) and Repetitive Extragenic Palindromic Polymerase Chain Reaction (REP-PCR),characterization of shigella spp. over two decades in Tianjin China. Int J Mol Epidemiol Genet 2012; 3(4):321-332.
Choi SY, Jeon YS, Lee JH, Choi B, Moon SH, von Seidlein L, Clemens JD, Dougan G, Wain J, Yu J, Lee JC, Seol SY, Lee BK, Song JH, Song M, Czerkinsky C, Chun J, Kim DW. Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries. J Med Microbiol. 2007; 56: 1460–1466.
Inouye M, Conway TC, Zobel J, Holt KE. Short read sequence typing (SRST): multi-locus sequence types from short reads. BMC Genomics 2012; 13: 338.
Wirth T, Falush D, Lan R, Colles F, Mensa P, Wieler LH,et al. Sex and virulence in Escherichia coli: an evolutionary perspective. Mol Microbiol 2006; 60:1136–1151.