Molecular investigation of CTX-M gene in Extended Spectrum β Lactamases (ESBLs) producing Pseudomonas aeruginosa isolated from Iranian patients with burn wound infection
Archives of Medical Laboratory Sciences,
Vol. 4 No. 1 (2018),
2 March 2019
https://doi.org/10.22037/amls.v4i1.23078
Abstract
Background: Pseudomonas aeruginosa (P. aeruginosa) is one of the most important causes of infection in burns and intensive care units. Extended-spectrum β-lactamases (ESBLs) production in P. aeruginosa is a major factor in the antibiotic resistance and is thought of as a serious threat to the currently available antibiotic armory. The purpose of this study was to determine the prevalence of CTX-M gene in ESBL-producing P. aeruginosa isolates in burn wound samples.
Materials and methods: In this cross-sectional survey, a total of 60 clinical isolates of P. aeruginosa were obtained from patients suffering from burn wound infection referred to major hospitals of Tehran, Iran. After verification by biochemical tests and antimicrobial susceptibility testing, CTX-M gene was identified using PCR method.
Results: The results of the molecular analysis of CTX-M gene showed that the prevalence of isolates of P. aeruginosa harboring CTX-M gene was 20% (12/60).
Conclusion: The results from this study showed high levels of antibiotic resistance and CTX-M gene among P. aeruginosa isolated samples of burn-wound infections which condition may result in the increased the emergence of multidrug-resistant strains and the failure of therapy This study suggests that detailed data on the CTX-M gene frequency can be useful to achieve the best therapy for infections caused by ESBLs producing P. aeruginosa.
- Pseudomonas aeruginosa
- CTX-M
- polymerase chain reaction
- antibiotic resistance
- ESBL
How to Cite
References
Peshattiwar PD , Peerapur BV. ESBL and MBL mediated resistance in Pseudomonas aeruginosa: An emerging threat to clinical therapeutics. J Clin Diagn Res 2011; 5: 1552-1554.
Chen Z, Niu H, Chen G, Li M, Li M,Zhou Y. Prevalence of ESBLs-producing Pseudomonas aeruginosa isolates from different wards in a Chinese teaching hospital. International journal of clinical and experimental medicine 2015; 8: 19400.
Fazeli N , Momtaz H. Virulence gene profiles of multidrug-resistant Pseudomonas aeruginosa isolated from Iranian hospital infections. Iranian Red Crescent Medical Journal 2014; 16.
Corehtash ZG, Ahmad Khorshidi FF, Akbari H,Aznaveh AM. Biofilm formation and virulence factors among Pseudomonas aeruginosa isolated from burn patients. Jundishapur journal of microbiology 2015; 8.
Tabar MM, Mirkalantari S,Amoli RI. Detection of ctx-M gene in ESBL-producing E. coli strains isolated from urinary tract infection in Semnan, Iran. Electronic physician 2016; 8: 2686.
Ibrahim A-S , Youssef N. Prevalence of CTX-M, TEM and SHV beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae isolated from Aleppo University Hospitals, Aleppo, Syria. Archives of Clinical Infectious Diseases 2015; 10.
Paterson DL , Bonomo RA. Extended-spectrum β-lactamases: a clinical update. Clinical microbiology reviews 2005; 18: 657-686.
Ghaima KK, Abdulhassan AA,Mahdi ZH. MOLECULAR STUDY OF EXTENDED SPECTRUM BETA-LACTAMASE (ESBL) GENES IN PSEUDOMONAS AERUGINOSA ISOLATED FROM BURNS.
Basak S, Attal RO,Rajurkar M, Pseudomonas aeruginosa and newer β-lactamases: an emerging resistance threat, in Infection Control-Updates. 2012, InTech.
Tzouvelekis L, Tzelepi E, Tassios P,Legakis N. CTX-M-type β-lactamases: an emerging group of extended-spectrum enzymes. International journal of antimicrobial agents 2000; 14: 137-142.
Ruppé E, Hem S, Lath S, Gautier V, Ariey F, Sarthou J-L, Monchy D,Arlet G. CTX-M β-lactamases in Escherichia coli from community-acquired urinary tract infections, Cambodia. Emerging infectious diseases 2009; 15: 741.
Slama TG. Gram-negative antibiotic resistance: there is a price to pay. Critical Care 2008; 12: S4.
Peleg AY , Hooper DC. Hospital-acquired infections due to gram-negative bacteria. New England Journal of Medicine 2010; 362: 1804-1813.
Weinstein RA , Mayhall CG. The epidemiology of burn wound infections: then and now. Clinical Infectious Diseases 2003; 37: 543-550.
Church D, Elsayed S, Reid O, Winston B,Lindsay R. Burn wound infections. Clinical microbiology reviews 2006; 19: 403-434.
Gangemi EN, Gregori D, Berchialla P, Zingarelli E, Cairo M, Bollero D, Ganem J, Capocelli R, Cuccuru F,Cassano P. Epidemiology and risk factors for pathologic scarring after burn wounds. Archives of facial plastic surgery 2008; 10: 93-102.
Ahmed OI, El-Hady SA, Ahmed TM,Ahmed IZ. Detection of bla SHV and bla CTX-M genes in ESBL producing Klebsiella pneumoniae isolated from Egyptian patients with suspected nosocomial infections. Egyptian Journal of Medical Human Genetics 2013; 14: 277–283.
Mesaros N, Nordmann P, Plésiat P, Roussel-Delvallez M, Van Eldere J, Glupczynski Y, Van Laethem Y, Jacobs F, Lebecque P,Malfroot A. Pseudomonas aeruginosa: resistance and therapeutic options at the turn of the new millennium. Clinical microbiology and infection 2007; 13: 560-578.
Parajuli NP, Maharjan P, Joshi G,Khanal PR. Emerging perils of extended spectrum β-lactamase producing enterobacteriaceae clinical isolates in a teaching hospital of Nepal. BioMed research international 2016; 2016.
Moghaddam MN, Beidokhti MH, Jamehdar SA,Ghahraman M. Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction. Iranian journal of basic medical sciences 2014; 17: 378.
Polotto M, Casella T, de Lucca Oliveira MG, Rúbio FG, Nogueira ML, de Almeida MT,Nogueira MC. Detection of P. aeruginosa harboring bla CTX-M-2, bla GES-1 and bla GES-5, bla IMP-1 and bla SPM-1 causing infections in Brazilian tertiary-care hospital. BMC infectious diseases 2012; 12: 176.
Peymani A, Naserpour-Farivar T, Zare E,Azarhoosh K. Distribution of blaTEM, blaSHV, and blaCTX-M genes among ESBL-producing P. aeruginosa isolated from Qazvin and Tehran hospitals, Iran. Journal of preventive medicine and hygiene 2017; 58: E155.
Ahmed OB, Asghar AH,Bahwerth FS. Prevalence of ESBL genes of Pseudomonas aeruginosa strains isolated from Makkah Hospitals, Saudi Arabia. Euro J Biol Med Sci Res 2015; 3: 12-18.
El-Shouny WA, Ali SS, Sun J, Samy SM,Ali A. Drug resistance profile and molecular characterization of extended spectrum beta-lactamase (ESβL)-producing Pseudomonas aeruginosa isolated from burn wound infections. Essential oils and their potential for utilization. Microbial pathogenesis 2018; 116: 301-312.
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