The Effect of Phytosterols and Fatty Acids of Pistachio (Pistacia vera) Oil on Spermatogenesis and Histological Testis Changes in Wistar Adult Male Rats The effect of pistachio oil on spermatogenesis
Vol. 19 No. 01 (2022),
18 March 2022
Purpose: Oilseeds and their related products are known to have various bioactive and health-promoting ingredients. In this research, we investigated the effects of phytosterols and fatty acids of Pistacia vera on spermatogenesis process and testis histological changes in Wistar male rats for the first time.
Materials and Methods: A total number of 64 adult male Wistar rats were divided randomly into eight groups including one control group, and seven test groups. Test groups received phytosterols, fatty acids, and pistachio oil orally for 30 days. Then, LH, FSH, and serum testosterone levels were determined. Also, the spermatogenesis process and changes in testicular tissue in rats were investigated.
Results: The results of this research suggest that phytosterols in doses of 10 and 50 mg/kg reduce the spermatogenesis process. Fatty acid in a low dose of 10 mg/kg increases spermatogenesis, but when a high dose of 50 mg/kg was used, it harmed the spermatogenesis process. When low levels of phytosterols and fatty acids are used simultaneously in dose 5 mg/kg, improvement in spermatogenesis process is observed but when these were used together in the dose of 25 mg/kg, the spermatogenesis process was disrupted. Using pistachio oil alone also improved spermatogenesis process.
Conclusion: It seems that phytosterols reduce spermatogenesis at high and low doses, while fatty acids increase spermatogenesis when used in low doses and reduce this process when used in high doses. The use of fatty acids extracted from pistachios to treat infertility in men seems hopeful.
- Infertility; Nut; Spermatogonia; Spermatid; Seminiferous tubules; sertoli cells; epithelial layer thickness
How to Cite
Krausz C, Forti G. Clinical aspects of male infertility. In The genetic basis of male infertility, 2000. pp. 1-21: Springer.
Bansal AK, Cheema RS. Role of Life Style Factors in Male Reproductive Functions: A Review. Theriogenology Insight-An International Journal of Reproduction in all Animals. 2019: 9: 111-116.
Hu FB, Stampfer MJ, Manson JE et al. Frequent nut consumption and risk of coronary heart disease in women: prospective cohort study. Bmj. 1998; 317: 1341-1345.
Grundy SM, Florentin L, Nix D et al. Comparison of monounsaturated fatty acids and carbohydrates for reducing raised levels of plasma cholesterol in man. Am J Clin Nutr. 1988; 47: 965-969.
Abdolshahi A, Mortazavi S, Naibandi S et al. Effect of solvent and extraction techniques on the fatty acid composition of Pistachio oil. Iran Food Sci Technol J 2014; 10.
Ozrenk K, Javidipour I, Yarilgac T et al. Fatty acids, tocopherols, selenium and total carotene of pistachios (P. vera L.) from Diyarbakir (Southeastern Turkey) and walnuts (J. regia L.) from Erzincan (Eastern Turkey). Food Sci Technol Int . 2012; 18: 55-62.
Maran R, Arunakaran J, Aruldhas M. Prolactin and Leydig cells: biphasic effects of prolactin on LH‐, T3‐and GH‐induced testosterone/oestradiol secretion by Leydig cells in pubertal rats. Int J Androl. 2001; 4: 48-55.
Clinton SK, Mulloy AL, Li SP et al. Dietary fat and protein intake differ in modulation of prostate tumor growth, prolactin secretion and metabolism, and prostate gland prolactin binding capacity in rats. J Nutr. 1997; 127: 225-237.
Abe M, Ito Y, Oyunzul L et al. Pharmacologically relevant receptor binding characteristics and 5α-reductase inhibitory activity of free fatty acids contained in saw palmetto extract. Biol. Pharm. Bull. 2009; 32: 646-650.
Yahyavi F, Alizadeh-Khaledabad M, Azadmard-Damirchi S. Oil quality of pistachios (Pistacia vera L.) grown in East Azarbaijan, Iran. NFS Journal. 2020; 18: 12-18.
Abidi S, List G, Rennick K. Effect of genetic modification on the distribution of minor constituents in canola oil. JAOCS. 1999; 7: 463-467.
Ryan E, Galvin K, O'connor T et al. Fatty acid profile, tocopherol, squalene and phytosterol content of brazil, pecan, pine, pistachio and cashew nuts. Int J Food Sci Nutr. 2006; 57: 219-228.
Bozorgi M, Memariani Z, Mobli M et al. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): a review of their traditional uses, phytochemistry, and pharmacology. Sci World J. 2013.
Satil F, Azcan N, Baser K. Fatty acid composition of pistachio nuts in Turkey. Chem. Nat. Compd. 2003; 39: 322-324.
Arena E, Campisi S, Fallico B et al. Distribution of fatty acids and phytosterols as a criterion to discriminate geographic origin of pistachio seeds. Food Chem. 2007; 104: 403-408.
Phillips KM, Ruggio DM, Ashraf-Khorassani M. Phytosterol composition of nuts and seeds commonly consumed in the United States. J Agric Food Chem. 2005; 53: 9436-9445.
Aslan M, Orhan I, Şener B. Comparison of the seed oils of Pistacia vera L. of different origins with respect to fatty acids. Int J Food Sci Technol. 2002; 37: 333-335.
Prager N, Bickett K, French N et al. A randomized, double-blind, placebo-controlled trial to determine the effectiveness of botanically derived inhibitors of 5-α-reductase in the treatment of androgenetic alopecia. J Altern Complement Med. 2002; 8: 143-152.
Ottestad I, Ose L, Wennersberg MH et al. Phytosterol capsules and serum cholesterol in hypercholesterolemia: a randomized controlled trial. Atherosclerosis. 2013; 228: 421-425.
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