the Short Abstinence May Have Paradoxical Effects On Sperms With Different Level Of DNA Integrity: A Prospective Study
Urology Journal,
Vol. 18 No. 06 (2021),
18 January 2022
,
Page 682-687
https://doi.org/10.22037/uj.v18i06.6515
Abstract
Purpose: To investigate the effect of short abstinence on sperm function tests and semen parameters.
Materials and methods: This prospective study included 65 male patients with increased DNA injury in their
ejaculated sperm and a history of recurrent pregnancy loss and/or assisted reproductive techniques failures. The effects of antioxidants medical therapy and short abstinence on semen quality were assessed (TUNEL test and CMA3 staining).
Results: Antioxidants have statistically significant effects on mean sperm concentration (untreated, 67.51 ± 44.40 million/ml, vs. treated, 56.09 ± 37.85 million/ml; P-value=0.005) and mean TUNEL score (untreated, 24.56% ± 9.49%, vs. treated, 20.64% ± 10.28%; P-value = 0.013). Moreover, a short abstinence period might have positive effects as shown on the TUNEL assay (20.64% ± 10.28 vs. 17.38% ± 8.59 ; P-value = 0.028) and CMA3 staining (47.79% ± 20.78, vs. short 41.92% ± 18.49; P-value = 0.019), when considering all study subjects. However, different results were obtained using more precise analysis based on a TUNEL cutoff score of 20%. The analysis showed that short abstinence might improve sperm DNA integrity in patients with TUNEL score > 20% (mean TUNEL score from 27.85% ± 8.32% to 19.14% ± 8.90% ; P-value =0.001%). However, it might have deleterious effects on sperm DNA integrity in patients with TUNEL score < 20% (mean TUNEL score from 11.89% ± 3.21% to 15.17% ± 7.79%; P-value = 0.045%)
Conclusion: Our results showed that short abstinence may not be beneficial in all infertile males, and it should
only be used in selected patients with abnormal DNA integrity.
- short abstinence, sperm DNA integrity, TUNEL, CMA3
How to Cite
References
Agarwal A, Majzoub A, Esteves SC, Ko E, Ramasamy R, Zini A (2016b) Clinical utility of sperm DNA fragmentation testing: practice recommendations based on clinical scenarios. Transl Androl Urol 5:935-950
Bansal AK, Bilaspuri GS (2010) Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2010
Borges E, Jr., Braga D, Zanetti BF, Iaconelli A, Jr., Setti AS (2019) Revisiting the impact of ejaculatory abstinence on semen quality and intracytoplasmic sperm injection outcomes. Andrology 7:213-219
Chen SJ, Allam JP, Duan YG, Haidl G (2013) Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches. Arch Gynecol Obstet 288:191-199
Cho CL, Agarwal A, Majzoub A, Esteves SC (2017) Future direction in sperm DNA fragmentation testing. Transl Androl Urol 6:S525-S526
Comar VA, Petersen CG, Mauri AL, Mattila M, Vagnini LD, Renzi A, Petersen B, Nicoletti A, Dieamant F, Oliveira JBA, Baruffi RLR, Franco JG, Jr. (2017) Influence of the abstinence period on human sperm quality: analysis of 2,458 semen samples. JBRA Assist Reprod 21:306-312
Cooper TG, Noonan E, von Eckardstein S, Auger J, Baker HW, Behre HM, Haugen TB, Kruger T, Wang C, Mbizvo MT, Vogelsong KM (2010) World Health Organization reference values for human semen characteristics. Hum Reprod Update 16:231-245
De Jonge C, LaFromboise M, Bosmans E, Ombelet W, Cox A, Nijs M (2004) Influence of the abstinence period on human sperm quality. Fertil Steril 82:57-65
Duran EH, Morshedi M, Taylor S, Oehninger S (2002) Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 17:3122-3128
Elzanaty S, Malm J, Giwercman A (2005) Duration of sexual abstinence: epididymal and accessory sex gland secretions and their relationship to sperm motility. Hum Reprod 20:221-225
Gosalvez J, Gonzalez-Martinez M, Lopez-Fernandez C, Fernandez JL, Sanchez-Martin P (2011) Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate. Fertil Steril 96:1083-1086
Greco E, Scarselli F, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Franco G, Anniballo N, Mendoza C, Tesarik J (2005) Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod 20:226-230
Lamb DJ (2010) Semen analysis in 21st century medicine: the need for sperm function testing. Asian J Androl 12:64-70
Marshburn PB, Giddings A, Causby S, Matthews ML, Usadi RS, Steuerwald N, Hurst BS (2014) Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation. Fertil Steril 102:705-710
Mayorga-Torres BJ, Camargo M, Agarwal A, du Plessis SS, Cadavid AP, Cardona Maya WD (2015) Influence of ejaculation frequency on seminal parameters. Reprod Biol Endocrinol 13:47
Mayorga-Torres JM, Agarwal A, Roychoudhury S, Cadavid A, Cardona-Maya WD (2016) Can a Short Term of Repeated Ejaculations Affect Seminal Parameters? J Reprod Infertil 17:177-183
Menezo Y, Entezami F, Lichtblau I, Belloc S, Cohen M, Dale B (2014) Oxidative stress and fertility: incorrect assumptions and ineffective solutions? Zygote 22:80-90
Menezo YJ, Hazout A, Panteix G, Robert F, Rollet J, Cohen-Bacrie P, Chapuis F, Clement P, Benkhalifa M (2007) Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. Reprod Biomed Online 14:418-421
Moskovtsev SI, Alladin N, Lo KC, Jarvi K, Mullen JB, Librach CL (2012) A comparison of ejaculated and testicular spermatozoa aneuploidy rates in patients with high sperm DNA damage. Syst Biol Reprod Med 58:142-148
O'Brien CM (2008) Sample Size Calculations in Clinical Research, Second Edition by Shein-Chung Chow, Jun Shao, Hansheng Wang. International Statistical Review 76:301-302
Pons I, Cercas R, Villas C, Brana C, Fernandez-Shaw S (2013) One abstinence day decreases sperm DNA fragmentation in 90 % of selected patients. J Assist Reprod Genet 30:1211-1218
Sakkas D, Urner F, Bizzaro D, Manicardi G, Bianchi PG, Shoukir Y, Campana A (1998) Sperm nuclear DNA damage and altered chromatin structure: effect on fertilization and embryo development. Hum Reprod 13 Suppl 4:11-19
Sanchez-Martin P, Sanchez-Martin F, Gonzalez-Martinez M, Gosalvez J (2013) Increased pregnancy after reduced male abstinence. Syst Biol Reprod Med 59:256-260
Schulte RT, Ohl DA, Sigman M, Smith GD (2010) Sperm DNA damage in male infertility: etiologies, assays, and outcomes. J Assist Reprod Genet 27:3-12
Sharma RK, Sabanegh E, Mahfouz R, Gupta S, Thiyagarajan A, Agarwal A (2010) TUNEL as a test for sperm DNA damage in the evaluation of male infertility. Urology 76:1380-1386
Shen ZQ, Shi B, Wang TR, Jiao J, Shang X, Wu QJ, Zhou YM, Cao TF, Du Q, Wang XX, Li D (2018) Characterization of the Sperm Proteome and Reproductive Outcomes with in Vitro Fertilization after a Reduction in Male Ejaculatory Abstinence Period. Mol Cell Proteomics
Simon L, Zini A, Dyachenko A, Ciampi A, Carrell DT (2017) A systematic review and meta-analysis to determine the effect of sperm DNA damage on in vitro fertilization and intracytoplasmic sperm injection outcome. Asian J Androl 19:80-90
Talebi AR, Moein MR, Tabibnejad N, Ghasemzadeh J (2008) Effect of varicocele on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical tests. Andrologia 40:245-251
Uppangala S, Mathai SE, Salian SR, Kumar D, Singh VJ, D'Souza F, Kalthur G, Kamath A, Adiga SK (2016) Sperm Chromatin Immaturity Observed in Short Abstinence Ejaculates Affects DNA Integrity and Longevity In Vitro. PLoS One 11:e0152942
Vahidi S, Moein M, Nabi A, Narimani N (2018) Effects of microsurgical varicocelectomy on semen analysis and sperm function tests in patients with different grades of varicocele: Role of sperm functional tests in evaluation of treatments outcome. Andrologia:e13069
Wein AJ, Kavoussi LR, Partin AW, Peters CA (2015) Campbell-Walsh Urology. Elsevier Health Sciences
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