The Effect of L-Carnitine and Coenzyme Q10 on the Sperm Motility, DNA Fragmentation, Chromatin Structure and Oxygen Free Radicals During, before and after Freezing in Oligospermia Men
Vol. 18 No. 03 (2021),
21 July 2021
Purpose: The aim of the present study is to assess the effect of L-carnitine and Coenzyme Q10 (CoQ10) on human sperm motility, DNA fragmentation, chromatin structure, and reactive oxygen species (ROS) during, before and after freezing in oligospermia men.
Materials and Methods: Semen was collected from 30 oligospermic men, who referred to infertility clinic of Beasat Hospital in Sanandaj, Iran. The samples of each individual were divided into 8 equal parts: 1. control group before freezing; 2. incubated with L-carnitine; 3. incubated with coenzyme Q10; 4. incubated with the combination of L-carnitine + CoQ10; 5. control freezing group; 6. the experimental freezing group with L-carnitine; 7. the experimental freezing group with coenzyme Q10 and 8. the experimental freezing with the combination of L-c + CoQ10. Sperm motility was assessed by WET MOUNT method. DNA fragmentation was evaluated by SCD (Sperm Chromatin Desperation), ROS, was evaluated by quantitative fluorescence reaction, and chromatin deficiency was determined by chromatin staining (CMA3).
Results: Antioxidant treatments, significantly reduced the number of ROS + in the pre and post freezing groups. Significant improvement was seen in the sperm motility of class B in the pre freezing groups with L-carnitine. Antioxidants also reduced the percentage of DNA fragmentation and protamine deficiency in pre-and post-freezing.
Conclusion: Addition of Coq10 and L-carnitine to human sperm medium significantly reduced the number of ROS. This reduction in ROS reduced sperm damage during cryopreservation.
- Freezing, L-carnitine, COQ10, DNA fragmentation, Protamine deficiency, ROS.
How to Cite
2- Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancy after intracytoplasmic injection of a single spermatozoon into an oocyte. Lancet. 1992; 340(8810): 14-18.
3- Renata W, Jan Karol W, and Jolanta S. The role of oxidative stress and antioxidants in male fertility. Cent European J Urol. 2013; 66(1): 60–67.
4- Verheyen G, De Croo I, Tournaye H, Pletincx I, Devroey P, van Steirteghem AC. Comparison of four mechanical methods to retrieve spermatozoa from testicular tissue. Hum Reprod. 1995; 10(11): 2956-9.
5- Erol B, Bozlu M, Hanci V, Tokgoz H, Bektas S, Mungan G. Coenzyme Q10 treatment reduces lipid peroxidation, inducible and endothelial nitric oxide synthases, and germ cell-specific apoptosis in a rat model of testicular ischemia/reperfusion injury. Fertil Steril. 2010; 93(1):280–282.
6- Balercia G, Buldreghini E, Vignini A, Tiano L, Paggi F, Amoroso S, et al. Coenzyme Q10 treatment in infertile men with idiopathic asthenozoospermia: a placebo-controlled, double-blind randomized trial. Fertil Steril 2009; 91(5):1785–1792.
7- Mohammad E, Alixides F and Mohammed F. Reactive Oxygen Species and Antioxidant in Seminal Plasma and their impact on Male Fertility. Int J Fertil Steril. 2009; 3(3), 87-110.
8- Talevi R, Barbato V, Fiorentino I, Braun S. Protective effects of in vitro treatment with zinc, d-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. REPROD BIOL ENDOCRIN. 2013; 1(11):81-91
9- Safarinejad MR. Efficacy of coenzyme Q10 on semen parameters, sperm function and reproductive hormones in infertile men. J Urol 2009; 182 (1):237-48.
10- Moslemi Mehni N, Ketabchi A and Hosseini E. Combination effect of Pentoxifylline and L-carnitine on idiopathic oligoasthenooterratozoosp. Iran J Reprod Med. 2014; 12)12(: 817-824.
11- Jeulin C, Lewin LM. Role of free L-carnitine and Acetyl-L-carnitine in post-gonadal maturation of spermatozoa. HUM REPROD UPDATE. 1996; 2(2): 87-102
12- Beydola T, Sharma RK, Agarwal A. Sperm preparation and selection techniques. J Reprod Infertil. 2014; 15(2):117–119.
13- Jameel T. Sperm swim-up: a simple and effective technique of semen processing for intrauterine insemination. J Pak Med Assoc. 2008; 58(2):71-74.
14- Di Santo M, Tarozzi N, Nadalini M and Borini A. Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART. Adv Urol. 2012; 5(1): 22-34
15- Taylor CT. Antioxidants and reactive oxygen species in human fertility. Environ Toxicol Pharmacol. 2001; 10(4): 189-98.
16- Fernandez JL, Muriel L, Goyanes V, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil. Steril. 2005; 84(4):833–42.
17- De Lamirande E, Gagnon C. Reactive oxygen species and human spermatozoa. II. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J Androl. 1992; 13 (5):379-386.
18- Hammadeh ME, Montenarh M and Fischer C. Protamine contents and P1/P2 ration in human spermatozoa from smoke and nonsmoker. Hum. Reprod. 2010; 11(25):2708–2720.
19- Nasr-Esfahani MH, Abasi H, Razavi S, AshrafiS, Tavalaee M. 2009.Varicocelectomy: semen parameters and protamine deficiency. Int JAndrol; 32(2): 115-22.
20- Talevi, R.; Barbato, V.; Fiorentino, I.; Braun, S.; Longobardi, S. and Gualtieri, R. (2013). Protective effects of in vitro treatment with zinc, daspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. Reproductive Biology and Endocrinology, 11:81.
21- Wail Wafa. Saeed AM, El-Nagar HA, Hussein YS. Effect of Coenzyme Q10 as an Antioxidant Added to Semen Extender During Cryopreservation of Buffalo and Cattle. J.Animal and Poultry Prod., Mansoura Univ., Vol.7 (11): 403 — 408,2016.
22- Melissa Rossi, Maria Elena Falomo & Roberto Mantovani (2016) Role ofcoenzyme Q and vitamin E on semen motility evaluated both in frozen and cooled- stored semen, Italian Journal of Animal Scienc.
23- Nawras N. Shams Alddin, Saad S. Al-Dujaily, Yahya K. Al-Sultani. In vitro sperm activation with pentoxifylline and L-carnitine for infertile men semen using layering and sedimentation techniques. Iraq J Embryos Infertil Res 2013; 3: 32-37.
24- Kadenisa,. January 2015. Effects of coenzyme Q10 added in freezing medium on sperm quality and DNA fragmentation after vitrification in normozoospermia.
25- Majida H.Mutlag Al-Shammari1, Jabbar H. Yenzeel1. Effect of L- Carnitine and COQ10 Addition to SMARTPro- Medium on Human SpermConcentration,SpermMorphology and Chromatin StructureduringIn VitroSperm Activation. IOSR Journal of Pharmacy and Biological Sciences (2017).
26- Ashok Agarwal and Ahmad Maizoub. Role of Antioxidants in Assisted Reproductive Techniques. Published online 2017 Apr 30. doi: 10.5534/wjmh.2017.35.2.77.
27- Mata-Campuzano M, Alvarez-Rodriguez M,Alvarez M, Anel L,Martinez-Pastor F (2012) Effect of several antioxidants on thawed ram spermatozoa submitted to 37 degrees C up to four hoursl. Reproduction in domestic animals = Zuchthygiene 47(6): 907-914.
- Abstract Viewed: 0 times
- 6400/pdf Downloaded: 0 times