IS there a Difference in Platelet-Rich Plasma Application Method and Frequency to Protect Against Urethra Stricture?
5 January 2019
Material-Method: Fifty rats were randomized into 5 groups, with 10 rats in each group. The urethras of all rats were traumatized with a pediatric urethrotome knife at 6 and 12 o’clock, except in the sham group. Group 1 was the sham group and had only urethral catheterization daily for 15 days, Group 2 was given 0.9% saline (physiologic saline [(UI+PS]) once a day after urethral injury (UI+ PS), Group 3 was injected with PRP submucosally after urethral injury, Group 4 was given PRP once a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury, and Group 5 was given PRP twice a day as intraurethral instillation using a 22 Ga catheter sheath with urethral injury.
Each administration of PRP was administered as 300 million platelets/150 microliters. On day 15, the penises of the rats were degloved to perform penectomy. Histopathologic evaluation was made for spongiofibrosis, inflammation, and congestion in vascular structures.
Results: When the sham group, UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared in total, there were significant differences identified for parameters other than edema. When the UI+PS, UI+PRPx1, UI+PRPx2 and UI+PRPs groups are compared, the UI+PS group was observed to have significantly more inflammation (mucosal inf. 2.42 ±0.53) and spongiofibrosis (2.42 ±0.53). All the PRP groups were identified to have significantly less mucosal inflammation (UI+PRPs 1±0, UI+PRPx1; 1.4±0.51, PRPx2; 1.33±0.5) and spongiofibrosis (UI+PRPs; 1.57±0.53, PRPx1; 1.2±0.42, PRPx2; 1.55±0.52). The group with lowest spongiofibrosis was seen to be the PRPx1 group.
Conclusion: This study showed that PRP significantly reduced mucosal inflammation and spongiofibrosis, independent of administration route, when applied to the urethra after urethral trauma
- Urethral Stricture
- Urethral Fibrozis
- Urethral inflamation
How to Cite
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