The Effects of Microfluidic Sperm Sorting, Density Gradient and Swim-up Methods on Semen Oxidation Reduction Potential
Vol. 17 No. 4 (2020),
24 June 2020
Purpose: To compare the effects of microfluidic sperm sorting, density gradient and swim-up methods on the oxidative reduction potential (ORP) of split semen samples from a single patient population.
Materials and Methods: A prospective controlled study was conducted to compare the effects of three different semen processing methods using split semen samples from the same population of infertile men. The primary outcome was the ORP. Secondary outcomes were the sperm concentration, progressive motility rate and total sperm motility.
Results: A total of 57 split semen samples were included in this study. The ORP was significantly lower in the
microfluidic group compared to the density gradient and swim-up groups (P < 0.05). The ORP/sperm concentration ratio was significantly lower in the microfluidic and density gradient groups compared to the swim-up group (P < 0.05). Total sperm concentration was significantly higher in the density gradient group than the microfluidic and swim-up groups (P < 0.05). Motility was significantly higher in the microfluidic and swim-up groups than the density gradient group (P < 0.05). The progressive motile sperm rate was significantly higher in the microfluidic and swim-up groups than the density gradient group (P < 0.05).
Conclusion: Microfluidic sperm sorting was better for selecting highly motile sperm and yielded a lower ORP than conventional sperm preparation methods.
Aitken RJ, Buckingham DW, Brindle J, Gomez E, Baker HW, Irvine DS. Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma. Hum Reprod. 1995;10: 2061–71.
Pasqualotto FF. Investigation and assisted reproduction in the treatment of male infertility. Rev Bras Ginecol Obstet. 2007; 29: 103-12.
Bisht S, Faiq M, Tolahunase M, Dada R. Oxidative stress and male infertility. Nat Rev Urol 2017; 14(8):470-485.
Brenda SC, Timothy GS, Xiaoyue Z, David C, Gary S, Shuichi T. Passively Driven Integrated Microfluidic System for Separation of Motile Sperm. Anal Chem. 2003; 75: 1671–1675.
Duck-bong S, Yuksel A, Feng ZC, Critser JK. Development of sorting, aligning, and orienting motile sperm using microfluidic device operated by hydrostatic pressure. Microfluid. Nanofluid 2007; 3: 561–570.
Quinn MM, Jalalian L, Ribeiro S, Ona K, Demirci U, Cedars MI, Rosen MP. Microfluidic sorting selects sperm for clinical use with reduced DNA damage compared to density gradient centrifugation with swim-up in split semen samples. Hum Reprod 2018; 33 (8): 1388-1393
Agarwal A, Gupta S, Sharma R. Oxidation-reduction potential measurement in ejaculated semen samples. In: Agarwal A, Gupta S, Sharma R, editors Andrological evaluation of male infertility: a laboratory guide. New York: Springer International Publishing: 2016. p. 165-170.
Agarwal A, Qiu E, Sharma R. Laboratory assessment of oxidative stress in semen. Arab Journal of Urology 2018; 16: 77-86.
Agarwal A, Wang SM. Clinical relevance of oxidation-reduction potential in the evaluation of male infertility. Urology 2017; 104:84-89.
Arafa M, Agarwal A, AI Said S, Maizoub A, Sharma R, Bjugstad KB, AlRumaihi K, Elbardisi H. Semen quality and infertility status can be identified through measures of oxidation reduction potential. Andrologia 2018; 50 (2).
Agarwal A, Arafa M, Chandrakumar R, Majzoub A, Alsaid S, Elbardisi H. A multicenter study to evaluate oxidative stress by oxidation-reduction potential a reliable and reproducible method Andrology; 2017; 5: 939-45.
Agarwal A, Roychoudhury S, Bjugstad KB, Cho CL. Oxidation reduction potential of semen: what is its role in the treatment of male infertility? Ther Adv Urol 2016; 8:302-318.
Esteves SC. Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. Int Braz J Urol 2014; 40:443-53.
Esteves SC, Hamada A, Kondray V, Pitchika A, Agarwal A. What every gynecologist should know about male infetility: an update. Arch Gynecol Obstet 2012; 286:217-29.
Esteves SC, Zini A, Aziz N, Alvarez JG, Sabanegh ES Jr, Agarwal A. Critical appraisal of World Health Organization’s new reference values for human semen caharacteristics and effect on diagnosis and treatment of subfertile men. Urology 2012; 79:16-22.
De Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm phsiology. Rev Reprod 1997;2: 48-54.
Du Plessis SS, Agarwal A, Halabi J, Tvrda E. Contemporary evidence on the physiological role of reactive oxygen species in human sperm function. J Assist Reprod Genet 2015; 32: 509-520.
Aitken RJ. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017; 84:1039-1052.
Zidi-Jrah I, Hajlaoui A, Mougou-Zerelli S, Kammoun M, Meniaoui I, Sallem A et.al. Realtionship between sperm aneuploidy, sperm DNA integrity, chromatin packaging, traditional senümen parameters, and recurrent pregnancy loss. Fertil Steril 2016; 105: 58-64.
Dinelli L, Courbiere B, Jouve E, Deveze C, Gnisci A, Grillo JM, Paulmyer-Lacroix O. Prognosis factors of pregnancy after intrauterine insemination with husband’s sperm: conclusions of an analysis of 2019 cycles. Fertil Steril 2014; 101(4):994-1000.
Twigg J, Irvine DS, Houston P, Fulton N, Micheal L, Aitken RJ. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation : protective significance of seminal plasma. Mol Hum Reprod 1998; 4:439-445.
Moskovtsev SI, Willis J, White J, Mullen JB. Sperm DNA damage: correlation to severity of semen abnormalities. Urology. 2009; 74(4): 789-93.
Oguz Y, Guler I, Erdem A, Mutlu MF, Gumuslu S, Oktem M, Bozkurt N, Erdem M. The effect of swim-up and gradient sperm preparation techniques on deoxyribonucleic acid (DNA) fragmentation in subfertile patients. J Assist Reprod Genet 2018; 35(6):1083-1089.
Agarwal A, Cho CL, Esteves SC. Should we evaluate and treat sperm DNA fragmentation? Curr Opin Obstet Gynecol 2016; 28:164-171
Zini A. Are sperm chromatin and DNA defects relevant in the clinic? Sys Biol Reprod Med 2011; 57:78-85.
Bungum M, Humaidan P, Axmon A, Spano M, Bungum L, Erenpreiss J, Giwercman A. Sperm DNA integrity assessment in prediction of assisted reproductive technology outcome. Hum Reprod 2007; 22(1):174-179.
Duran EH, Morshedi M, Taylor S, Oehninger S. Sperm DNA quality predicts intrauterine insemination outcome: a prospective cohort study. Hum Reprod 2002; 17:3122-3128.
Spano M, Bonde JP, Hjollund HI, Kolstad HA, Cordelli E, Letter G. The Danish First Pregnancy Planner Study Team. Sperm chromatin damage impairs human fertility. Fertil Steril 2000; 73:43-50.
Lin MH, Kuo-Kuang Lee R, Li SH, Lu CH, Sun FJ, Hwu YM. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in invitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril 2008; 90(2):352-9.
Twigg J, Irvine DS, Houston P, Fulton N, Micheal L, Aitken RJ. Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma. Mol Hum Reprod 1998; 4:439-445.
Amiri I, Ghorbani M, Heshmati S. Comparison of the DNA fragmentation and the sperm parameters after processing by the density gradient and the swim up methods. J Clin Diagn Res 2012; 6(9):1451-3.
Jayaraman V, Upahya D, Narayan PK, Adiga SK. The sperm processing by swim-up and density gradient is effective in the elimination of the sperm with DNA damage. J Assist Reprod Genet 2012; 29(6):557-63.
Xue X, Wang WS, Shi JZ, Zhang ZL, Zhao WQ, Shi WH et.al. Efficacy of swim-up versus density gradient centrifugation in improving sperm deformity rate and DNA fragmantation index in semen samples from teratozoospermic patients. J Assist Reprod Genet 2014; 31(9):1161-6.
Schulte R, Chung YK, Ohl DA, Takayama S, Smith GD. Microfluidic sperm sorting device provides a novel method for selectingmotile sperm with higher DNA integrity. Fertil Steril 2007; 88 (supp 1):576.
Asghar W, Velasco V, Kinsley JL, Shoukat MS, Shafiee H, Anchan RM, Mutter GL, Tüzel E, Demirci U. Selection of functional human sperm with higher DNA integrity and fewer reactive oxygen species. Adv Healthc Mater 2014; 3(10):1671-9.
Shirota K, Yotsumoto F, Itoh H, Obama H, Hidaka N, Nakajima K, Miyamoto S. Seperation efficiency of a microfluidic sperm sorter to minimize sperm DNA damage. Fertil Steril 2016; 105:315-321.
- Abstract Viewed: 77 times
- pdf/5639 Downloaded: 27 times